干旱区生态环境知识资源中心(Arid): 热融滑塌对青藏高原北麓河荒漠草原土壤细菌群落的影响

Arid

	热融滑塌对青藏高原北麓河荒漠草原土壤细菌群落的影响
其他题名	Effects of thaw slump on the bacterial community in a desert steppe in the Beiluhe Region of the Qinghai-Tibet Plateau
	张晓兰 1; 刘桂民 2; 徐海燕 2; 李新星 2; 吴小丽 2; 纪庚好 2; 李莉莎 2; 吴晓东 3
来源期刊	应用与环境生物学报
ISSN	1006-687X
出版年	2019
卷号	25 期号:6 页码:1327-1334
中文摘要	多年冻土退化会引起热融滑塌,进而对多年冻土区的生态系统产生影响,但热融滑塌对土壤细菌群落的影响还不清楚.研究区选择青藏高原中部的荒漠草原地区.利用Illumina测序技术,对土壤细菌的16S rRNA V3-V4高变区进行测序,在纲水平上分析了3种微地貌(对照区、滑塌区、沉降区)下表层0-30 cm土壤细菌的alpha多样性、物种丰度和组成,并结合土壤理化指标研究了影响细菌群落结构的环境因子.结果显示,土壤细菌在纲水平上共有91个细菌类群,放线菌纲(29.4%,Actinobacteria)、酸杆菌纲(14.16%,Acidobacteria)、alpha-变形菌纲(12.69%,Alphaproteobacteria)和芽单胞菌纲(6.92%,Gemmatimonadetes)是优势菌群,放线菌纲在各采样点相对丰度最高.热融滑塌改变了土壤含水量、全碳和有机碳等理化指标. Mantel测试和RDA分析表明,土壤全碳和含水量是影响细菌群落结构的关键环境因子;相关性分析表明,土壤含水量、电导率、全氮是影响细菌群落多样性和优势菌纲相对丰度的关键环境因子.本研究表明在荒漠草原地区,热融滑塌会降低土壤碳含量和酸杆菌纲的相对丰度,并对土壤细菌群落的结构、多样性及在冻土中的分布产生影响.
英文摘要	Permafrost degradation leads to the formation of thaw slumps, which further affects permafrost ecosystems. However, the effects of thaw slump on bacterial community remain largely unknown. For the research site, we selected a desert steppe area in the central Qinghai-Tibetan Plateau. The Illumina sequencing technique was used to examine the 16S rRNA V3-V4 hypervariable region of soil bacterial communities. We examined alpha diversity, relative abundances, and bacterial community composition at the class level in soils of three landform micromorphologies (control, collapsing, slumped). The soil physicochemical properties were also investigated in order to explore the main environmental factors affecting the bacterial community structure. In total, 91 classes were observed, among which the dominant classes were Actinobacteria (29.4%), Acidobacteria (14.16%), alpha-Proteobacteria (12.69%), and Gemmatimonadetes (6.92%). Actinobacteria were the most abundant in the soils of all three landforms. The results showed that thaw slump dramatically changed the physicochemical properties of the soil, including soil water content, total carbon, and organic carbon content. The Mantel test and RDA analysis showed that soil total carbon and water content were the main environmental factors affecting bacterial community structure. The correlation analysis showed that soil water content, conductivity, and total nitrogen were the driving factors of bacterial diversity and relative abundances of dominant classes. This study showed that thaw slump in desert steppe area decreased soil carbon contents and relative abundance of Acidobacteria. The soil bacterial structure, diversity, and distribution were also greatly affected by thaw slumps.
中文关键词	热融滑塌 ; 青藏高原 ; 荒漠草原 ; 细菌群落 ; 多年冻土 ; 高通量测序
英文关键词	thaw slump Qinghai-Tibetan Plateau desert steppe bacterial community permafrost high-throughput sequencing
语种	中文
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:6649995
来源机构	中国科学院西北生态环境资源研究院
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315868
作者单位	1.兰州交通大学环境与市政工程学院;;中国科学院西北生态环境资源研究院, ;;冰冻圈国家重点实验室青藏高原冰冻圈观测研究站, 兰州;;兰州, ;; 730070;;730000; 2.兰州交通大学环境与市政工程学院, 兰州, 甘肃 730070, 中国; 3.中国科学院西北生态环境资源研究院, 冰冻圈国家重点实验室青藏高原冰冻圈观测研究站, 兰州, 甘肃 730000, 中国
推荐引用方式 GB/T 7714	张晓兰,刘桂民,徐海燕,等. 热融滑塌对青藏高原北麓河荒漠草原土壤细菌群落的影响[J]. 中国科学院西北生态环境资源研究院,2019,25(6):1327-1334.
APA	张晓兰.,刘桂民.,徐海燕.,李新星.,吴小丽.,...&吴晓东.(2019).热融滑塌对青藏高原北麓河荒漠草原土壤细菌群落的影响.应用与环境生物学报,25(6),1327-1334.
MLA	张晓兰,et al."热融滑塌对青藏高原北麓河荒漠草原土壤细菌群落的影响".应用与环境生物学报 25.6(2019):1327-1334.