干旱区生态环境知识资源中心(Arid): 祁连山高寒草甸和荒漠草原土壤细菌群落的结构特征

Arid

	祁连山高寒草甸和荒漠草原土壤细菌群落的结构特征
其他题名	Characterization of soil bacterial communities in alpine and desert grasslands in the Qilian Mountain range
	康宝天; 侯扶江
来源期刊	草业科学
ISSN	1001-0629
出版年	2020
卷号	37 期号:1 页码:10-19
中文摘要	祁连山作为我国西部重要的生态区,分布有大量草地,探明祁连山不同草地类型土壤微生物群落组成及其影响因子,对该地区深入研究土壤生态系统结构和维持草地健康具有参考价值。本研究采用Illumina MiSeq高通量测序和OTU分析法比较了位于祁连山东缘青海省刚察县高寒草甸和河西走廊临泽县平山湖荒漠草原0-10 cm土层中土壤细菌群落结构的差异,同时测定土壤理化性质,探讨了土壤细菌群落结构与土壤环境因子之间的关系。结果表明:高寒草甸和荒漠草原土壤细菌的beta多样性和细菌群落组成存在显著差异;在门水平上,两种草地类型土壤细菌均以放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)和酸杆菌门(Acidobacteria)为主要类群,相对丰度分别占总数的84%和89%;放线菌门为两地优势类群,相对丰度分别为57%和29%。在门水平上,两种草地类型主要细菌类群相对丰度有差异,其中高寒草甸土壤放线菌门和绿弯菌门的相对丰度显著低于荒漠草原(P <0.05),而变形菌门、酸杆菌门、拟杆菌门(Bacteroidetes)和Rokubacteria反之(P <0.05);两草地类型间土壤芽胞单菌门(Gemmatimonadetes)、疣微菌门(Verrucomicrobia)、浮霉菌门(Planctomycetes)相对丰度无显著差异(P >0.05)。主要类群与环境因子的Pearson相关性及PCA主成分分析表明:土壤放线菌门和绿弯菌门相对丰度与土壤养分(全氮、全碳、有机碳、含水量和碳氮比)之间呈显著负相关关系(P <0.05),与容重呈显著正相关关系(P <0.05);土壤变形菌门,酸杆菌门和Rokubacteria的相对丰度与土壤养分呈显著正相关关系,与容重呈负相关关系(P <0.05)。土壤全碳、全氮、pH、有机碳、含水量、容重和碳氮比是影响两种草地类型土壤细菌菌群分布的重要原因。
英文摘要	The Qilian Mountain range is an important ecological zone in western China. Grasslands account for a significant proportion of land area in this mountain range. Understanding the composition of soil microbial communities in different grassland types found in the Qilian Mountains, as well as their relationship with soil factors, is important for sustainable conservation of grassland health. In the present study, we examined the diversity of soil bacterial communities using Illumina MiSeq high-throughput sequencing and investigated the relationship between bacterial composition and soil parameters in an alpine grassland meadow on the northeastern side of the Qilian Mountains and in a desert grassland in the Pingshan Lake desert (Linze County, Hexi Corridor). The beta diversity and community composition analyses revealed a significant difference between the bacterial communities in the two investigated grasslands. The four phyla that dominated both grasslands were Actinobacteria, Proteobacteria, Chloroflexi, and Acidobacteria. These phyla accounted for 84% and 89% of the total bacterial community in the alpine and desert grassland, respectively. Actinobacteria was the most dominant phylum in both grasslands, accounting for 57% and 29% of the total bacterial community in the desert and alpine grassland,respectively. The relative abundances of Actinobacteria and Chloroflexi were significantly higher in the alpine grassland than in the desert grassland (P <0.05). The relative abundances of Proteobacteria, Acidobacteria, Bacteroidetes, and Rokubacteria were significantly higher in the alpine grassland than in the desert grassland (P <0.05). There was no significant difference in the relative abundances of Gemmatimonadetes, Verrucomicrobia, and Planctomycetes between the two grasslands (P >0.05). Pearson correlation and principal component analysis showed that Actinobacteria had a significant negative correlation with soil nutrients (total nitrogen, total carbon, organic carbon, water content, and carbon:nitrogen ratio)(P <0.01)and a significant positive correlation with soil bulk density (P <0.01). Proteobacteria, Acidobacteria, and Rokubacteria were positively correlated with soil nutrients and negatively correlated with soil bulk density (P <0.05). Our results indicated that total soil nitrogen, total soil carbon, soil pH, organic carbon, water content, soil bulk density, and carbon:nitrogen ratio were the key determinants of soil bacterial community distribution at the depth of 0 - 10 cm in the two investigated grassland types.
中文关键词	祁连山 ; 高寒草甸 ; 荒漠草原 ; 土壤细菌群落 ; 土壤理化因子
英文关键词	Qilian Mountains alpine grassland desert grassland soil bacterial communities soil physical and chemical properties
语种	中文
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:6660954
来源机构	兰州大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315777
作者单位	兰州大学草地农业科技学院, 草地农业生态系统国家重点实验室, 兰州, 甘肃 730020, 中国
推荐引用方式 GB/T 7714	康宝天,侯扶江. 祁连山高寒草甸和荒漠草原土壤细菌群落的结构特征[J]. 兰州大学,2020,37(1):10-19.
APA	康宝天,&侯扶江.(2020).祁连山高寒草甸和荒漠草原土壤细菌群落的结构特征.草业科学,37(1),10-19.
MLA	康宝天,et al."祁连山高寒草甸和荒漠草原土壤细菌群落的结构特征".草业科学 37.1(2020):10-19.