干旱区生态环境知识资源中心(Arid): Polyphasic characterization of nitrogen-fixing and co-resident bacteria in nodules of Phaseolus lunatus inoculated with soils from Piaui State, Northeast Brazil

Arid

DOI	10.1007/s13199-020-00672-1
	Polyphasic characterization of nitrogen-fixing and co-resident bacteria in nodules of Phaseolus lunatus inoculated with soils from Piaui State, Northeast Brazil
	Chibeba, Amaral Machaculeha 1; Pereira, Claudiana Silva 2; Antunes, Jadson Emanuel Lopes 2; Ribeiro, Renan Augusto 3; Lopes, Angela Celis de Almeida 3,4; Gomes, Regina Lucia Ferreira 3,4; Hungria, Mariangela 2,5; Araujo, Ademir Sergio Ferreira 2,3
通讯作者	Hungria, Mariangela
来源期刊	SYMBIOSIS
ISSN	0334-5114
EISSN	1878-7665
出版年	2020
卷号	80 期号:3 页码:279-292
英文摘要	Phaseolus lunatus (Lima bean) is an important legume for the poor population of the Brazilian northeast region. The legume is able to take advantage of the nitrogen fixation process, but the diversity of indigenous microsymbionts is poorly known. In this study, 29 bacteria isolated from root nodules of P. lunatus inoculated under greenhouse conditions with soils from Piaui State, in the northeast semi-arid region of Brazil, were obtained and characterized. Classical morphological and biochemical essays revealed high phenotypic diversity, splitting the bacteria into four clusters. Genetic fingerprinting by BOX-PCR indicated outstanding diversity, with the 29 strains positioned in 19 different clusters with 30% of final similarity. Nine genera were confirmed in the analysis of the 16S rRNA gene, with two typical nodulating N-2-fixing clades, Bradyrhizobium and Agrobacterium/Rhizobium, accounting for 38% and 21% of the isolates, respectively; the results were confirmed with the housekeeping gyrB gene, that also indicated putative new species. Bradyrhizobium was confirmed as the main symbiont, being present in nodules of all plants. All rhizobia except for those with higher resemblance to agrobacteria carry nifH genes. Six other genera were isolated as nodules endophytes, Bacillus, Burkholderia, Enterobacter, Franconibacter, Pseudomonas and Williamsia. Several of these endophytes exhibited one or more important biochemical properties, such as the synthesis of catalase, gelatinase and the ability to solubilize phosphate, that might confer ecological advantages to the rhizobia associated with them in the harsh environment of the Brazilian semi-arid, explaining the high rate of co-infection detected in the nodules.
英文关键词	Biological nitrogen fixation Lima bean Nodulation Biodiversity
类型	Article
语种	英语
国家	Mozambique ; Brazil
收录类别	SCI-E
WOS记录号	WOS:000523084500001
WOS关键词	BRADYRHIZOBIUM STRAINS ; SP NOV. ; RHIZOBIA ; GROWTH ; CLASSIFICATION ; NODULATION ; SYMBIONTS ; INCREASE ; ALFALFA
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315643
作者单位	1.IITA, Nampula, Mozambique; 2.Univ Fed Piaui, Agr Sci Ctr, Soil Qual Lab, Teresinha, PI, Brazil; 3.CNPq, SHIS QI 1 Conjunto B,Blocos ABC & D, Brasilia, DF, Brazil; 4.Univ Fed Piaui, Dept Plant Sci, Agr Sci Ctr, Teresinha, PI, Brazil; 5.Embrapa Soja, Soil Biotechnol Lab, Londrina, PR, Brazil
推荐引用方式 GB/T 7714	Chibeba, Amaral Machaculeha,Pereira, Claudiana Silva,Antunes, Jadson Emanuel Lopes,et al. Polyphasic characterization of nitrogen-fixing and co-resident bacteria in nodules of Phaseolus lunatus inoculated with soils from Piaui State, Northeast Brazil[J],2020,80(3):279-292.
APA	Chibeba, Amaral Machaculeha.,Pereira, Claudiana Silva.,Antunes, Jadson Emanuel Lopes.,Ribeiro, Renan Augusto.,Lopes, Angela Celis de Almeida.,...&Araujo, Ademir Sergio Ferreira.(2020).Polyphasic characterization of nitrogen-fixing and co-resident bacteria in nodules of Phaseolus lunatus inoculated with soils from Piaui State, Northeast Brazil.SYMBIOSIS,80(3),279-292.
MLA	Chibeba, Amaral Machaculeha,et al."Polyphasic characterization of nitrogen-fixing and co-resident bacteria in nodules of Phaseolus lunatus inoculated with soils from Piaui State, Northeast Brazil".SYMBIOSIS 80.3(2020):279-292.