干旱区生态环境知识资源中心(Arid): The widely distributed legume tree Vachellia (Acacia) nilotica subsp. indica is nodulated by genetically diverse Ensifer strains in India

Arid

DOI	10.1007/s13199-019-00658-8
	The widely distributed legume tree Vachellia (Acacia) nilotica subsp. indica is nodulated by genetically diverse Ensifer strains in India
	Choudhary, Sunil 1,2; Tak, Nisha 1; Bissa, Garima 1; Chouhan, Bhawana 1; Choudhary, Priyanka 1; Sprent, Janet I.3; James, Euan K.4; Gehlot, Hukam S.1
通讯作者	Tak, Nisha
来源期刊	SYMBIOSIS
ISSN	0334-5114
EISSN	1878-7665
出版年	2020
卷号	80 期号:1 页码:15-31
英文摘要	Vachellia (syn. Acacia) nilotica subsp. indica is a widespread legume tree in India of ecological and economic importance due to its ability to nodulate and fix nitrogen. The structure of V. nilotica nodules was examined and diversity of its rhizobial symbionts in various locations in India was assessed. More than a hundred strains were purified from indeterminate Vachellia-type root nodules on seedlings grown in alkaline soils from different regions of Thar Desert in Western Rajasthan and in neighbouring states (Gujarat, Haryana, Punjab). Plants grown in acidic soils of Meghalaya and Nagaland failed to nodulate. Twenty-one strains identified as species of Ensifer (Sinorhizobium) based on their 16S rRNA gene sequences and grouped into seven recA-types. Eight strains were characterized using two additional housekeeping (glnII, dnaK) and symbiotic (nodA, nifH) genes. A concatenated core gene phylogeny revealed that V. nilotica-Ensifer strains are novel and genetically diversified from E. saheli, E. arboris, E. terangae and E. americanus. Most are distinct from Ensifer strains previously reported in mimosoid, caesalpinioid and papilionoid legume species of W. Rajasthan. These Ensifer strains harboured typical mimosoid-type symbiotic genes that were diversified from E. arboris, E. kostiensis and E. terangae. They nodulated other mimosoids (Prosopis cineraria, Mimosa hamata, Senegalia senegal and Vachellia spp.), but failed to make symbiotic interactions with a papilionoid crop species, Vigna radiata, suggesting their specificity towards mimosoids. Vachellia nilotica is quite promiscuous, fixing nitrogen in symbiosis with diverse strains of Ensifer, and has successfully invaded various agro-climatic regions, thus indicating that it could be exploited for agroforestry projects.
英文关键词	Ensifer Vachellia nilotica Nodulation Phylogeny Symbiotic and housekeeping genes
类型	Article
语种	英语
国家	India ; Scotland
收录类别	SCI-E
WOS记录号	WOS:000511911900002
WOS关键词	LEGUMINOUS TREES ; SYMBIOTIC CHARACTERIZATION ; SEQUENCE-ANALYSIS ; SP NOV. ; RHIZOBIA ; SINORHIZOBIUM ; NODULES ; CLASSIFICATION ; EVOLUTIONARY ; BACTERIA
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315642
作者单位	1.Jai Narain Vyas Univ, Ctr Adv Study, Dept Bot, BNF & Microbial Genom Lab, Jodhpur 342001, Rajasthan, India; 2.Govt Coll Jayal, Nagaur 341001, Rajasthan, India; 3.Univ Dundee, James Hutton Inst, Div Plant Sci, Dundee DD2 5DA, Scotland; 4.James Hutton Inst, Dundee DD2 5DA, Scotland
推荐引用方式 GB/T 7714	Choudhary, Sunil,Tak, Nisha,Bissa, Garima,et al. The widely distributed legume tree Vachellia (Acacia) nilotica subsp. indica is nodulated by genetically diverse Ensifer strains in India[J],2020,80(1):15-31.
APA	Choudhary, Sunil.,Tak, Nisha.,Bissa, Garima.,Chouhan, Bhawana.,Choudhary, Priyanka.,...&Gehlot, Hukam S..(2020).The widely distributed legume tree Vachellia (Acacia) nilotica subsp. indica is nodulated by genetically diverse Ensifer strains in India.SYMBIOSIS,80(1),15-31.
MLA	Choudhary, Sunil,et al."The widely distributed legume tree Vachellia (Acacia) nilotica subsp. indica is nodulated by genetically diverse Ensifer strains in India".SYMBIOSIS 80.1(2020):15-31.