干旱区生态环境知识资源中心(Arid): Tracing life at the dry limit using phospholipid fatty acids

Arid

DOI	10.1016/j.soilbio.2019.107661
	Tracing life at the dry limit using phospholipid fatty acids - does sampling matter?
	Kusch, Stephanie 1; Jaeschke, Andrea 1; Moerchen, Ramona 2; Rethemeyer, Janet 1
通讯作者	Kusch, Stephanie
来源期刊	SOIL BIOLOGY & BIOCHEMISTRY
ISSN	0038-0717
出版年	2020
卷号	141
英文摘要	Phospholipid fatty acids (PLFAs) have been used to trace bacterial life in extremely carbon-poor soils of the hyperarid Atacama Desert. However, the low abundances of bacteria and, thus, PLFAs increases the risk of contamination by exogenous PLFAs. Here, we assess whether field sampling strategies (super-clean, clean, and regular sampling protocols) have an effect on PLFA diversity and abundance in hyperarid Atacama soils or whether laboratory processing or true environmental heterogeneity control PLFA inventories. Our results show no exogenous PLFA contribution during sample processing in the lab and statistical analyses (ANOVA, Kruskal-Wallis) reveal that PLFA abundances do not differ significantly between replicate samples (n = 3) taken with the three different sampling strategies. Rather than sampling strategy, our results show that PLFA abundances in the investigated soil replicates rather reflect true environmental heterogeneity of primarily bacterial biomass (in the absence of indigenous fungi), potentially related to small-scale physicochemical differences.
英文关键词	Phospholipid fatty acids Atacama Desert Sampling Environmental heterogeneity
类型	Article
语种	英语
国家	Germany
收录类别	SCI-E
WOS记录号	WOS:000513986700005
WOS关键词	ARID ATACAMA DESERT ; MICROBIAL COMMUNITIES ; SOIL ; DIVERSITY ; RATIO
WOS类目	Soil Science
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315581
作者单位	1.Univ Cologne, Inst Geol & Mineral, Zulpicher Str 49b, D-50674 Cologne, Germany; 2.Inst Crop Sci & Resource Conservat INRES, Nussallee 13, D-53115 Bonn, Germany
推荐引用方式 GB/T 7714	Kusch, Stephanie,Jaeschke, Andrea,Moerchen, Ramona,et al. Tracing life at the dry limit using phospholipid fatty acids - does sampling matter?[J],2020,141.
APA	Kusch, Stephanie,Jaeschke, Andrea,Moerchen, Ramona,&Rethemeyer, Janet.(2020).Tracing life at the dry limit using phospholipid fatty acids - does sampling matter?.SOIL BIOLOGY & BIOCHEMISTRY,141.
MLA	Kusch, Stephanie,et al."Tracing life at the dry limit using phospholipid fatty acids - does sampling matter?".SOIL BIOLOGY & BIOCHEMISTRY 141(2020).