干旱区生态环境知识资源中心(Arid): miRNA-mRNA Integrated Analysis Reveals Roles for miRNAs in a Typical Halophyte, Reaumuria soongorica, during Seed Germination under Salt Stress

Arid

DOI	10.3390/plants9030351
	miRNA-mRNA Integrated Analysis Reveals Roles for miRNAs in a Typical Halophyte, Reaumuria soongorica, during Seed Germination under Salt Stress
	Zhang, Huilong 1,2; Liu, Xiaowei 1,2; Yang, Xiuyan 1,2; Wu, Haiwen 1,2; Zhu, Jianfeng 1,2; Zhang, Huaxin 1,2
通讯作者	Zhu, Jianfeng ; Zhang, Huaxin
来源期刊	PLANTS-BASEL
EISSN	2223-7747
出版年	2020
卷号	9 期号:3
英文摘要	MicroRNAs (miRNAs) are endogenous small RNAs that play a crucial role in plant growth, development, and environmental stress responses. Reaumuria soongorica is a typical halophyte that is widely distributed in saline-alkali desert regions. Under salt stress, R. soongorica can complete germination, a critical biological process in the life cycle of seed plants. To identify miRNAs and predict target mRNAs involved in seed germination during salt stress, nine small-RNA libraries were constructed and analyzed from R. soongorica seeds treated with various concentrations of NaCl. We also obtained transcriptome data under the same treatment conditions. Further analysis identified 88 conserved miRNAs representing 25 defined families and discovered 13 novel miRNAs from nine libraries. A co-expression analysis was performed on the same samples to identify putative miRNA-mRNA interactions that were responsive to salt stress. A comparative analysis of expression during germination under 273 (threshold) and 43 mM (optimal) NaCl treatments identified 13 differentially expressed miRNAs and 23 corresponding target mRNAs, while a comparison between 43 mM NaCl and non-salt-stress conditions uncovered one differentially expressed miRNA and one corresponding target mRNA. These results provide basic data for further study of molecular mechanisms involved in the germination of salt-stressed R. soongorica seeds, and also provide a reference for the improvement of salt tolerance during plant germination.
英文关键词	Reaumuria soongorica seed germination microRNA salt stress high-throughput sequencing
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000525315000010
WOS关键词	GENOME-WIDE IDENTIFICATION ; CATABOLIC GENE CYP707A2 ; GLUCOSE-INDUCED DELAY ; RESPONSIVE MICRORNAS ; EXPRESSION ; TARGETS ; REPRESSION ; EVOLUTION ; DORMANCY ; CHINA
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315352
作者单位	1.State Forestry & Grassland Adm, Res Ctr Saline & Alkali Land, Beijing 100091, Peoples R China; 2.Chinese Acad Forestry, Tianjin Res Inst Forestry, Tianjin 300450, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Huilong,Liu, Xiaowei,Yang, Xiuyan,et al. miRNA-mRNA Integrated Analysis Reveals Roles for miRNAs in a Typical Halophyte, Reaumuria soongorica, during Seed Germination under Salt Stress[J],2020,9(3).
APA	Zhang, Huilong,Liu, Xiaowei,Yang, Xiuyan,Wu, Haiwen,Zhu, Jianfeng,&Zhang, Huaxin.(2020).miRNA-mRNA Integrated Analysis Reveals Roles for miRNAs in a Typical Halophyte, Reaumuria soongorica, during Seed Germination under Salt Stress.PLANTS-BASEL,9(3).
MLA	Zhang, Huilong,et al."miRNA-mRNA Integrated Analysis Reveals Roles for miRNAs in a Typical Halophyte, Reaumuria soongorica, during Seed Germination under Salt Stress".PLANTS-BASEL 9.3(2020).