干旱区生态环境知识资源中心(Arid): Expression of Arabidopsis thaliana Thioredoxin-h2 in Brassica napus enhances antioxidant defenses and improves salt tolerance

Arid

DOI	10.1016/j.plaphy.2019.12.032
	Expression of Arabidopsis thaliana Thioredoxin-h2 in Brassica napus enhances antioxidant defenses and improves salt tolerance
	Ji, Myung Geun 1; Park, Hee Jin 2,6; Cha, Joon-Yung 1; Kim, Jin A.3; Shin, Gyeong-Im 1; Jeong, Song Yi 1; Lee, Eun Seon 4,5; Yun, Dae-Jin 6; Lee, Sang Yeol 1,4,5; Kim, Woe-Yeon 1,4,5
通讯作者	Kim, Woe-Yeon
来源期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
EISSN	1873-2690
出版年	2020
卷号	147 页码:313-321
英文摘要	Salt stress limits crop productivity worldwide, particularly in arid and heavily irrigated regions. Salt stress causes oxidative stress, in which plant cells accumulate harmful levels of reactive oxygen species (ROS). Thioredoxins (Trxs; EC 1.8.4.8) are antioxidant proteins encoded by a ubiquitous multigene family. Arabidopsis thaliana Trx h-type proteins localize in the cytoplasm and other subcellular organelles, and function in plant responses to abiotic stresses and pathogen attack. Here, we isolated the Arabidopsis genes encoding two cytosolic h-type Trx proteins, AtTrx-h2 and AtTrx-h3 and generated transgenic oilseed rape (Brassica napus) plants overexpressing AtTrx-h2 or AtTrx-h3. Heterologous expression of AtTrx-h2 in B. napus conferred salt tolerance with plants grown on 50 mM NaCI having higher fresh weight and chlorophyll contents compared with controls in hydroponic growth system. By contrast, expression of AtT'rx-h3 or the empty vector control did not improve salt tolerance. In addition, AtTrx-h2-overexpressing transgenic plants exhibited lower levels of hydrogen peroxide and higher activities of antioxidant enzymes including peroxidase, catalase, and superoxide dismutase, compared with the plants expressing the empty vector control or At7'rx-h3. These results suggest that AtTrx-h2 is a promising candidate for engineering or breeding crops with enhanced salt stress tolerance.
英文关键词	Antioxidant system Arabidopsis thaliana Brassica napus Heterologous expression Salt tolerance Thioredoxin-h2
类型	Article
语种	英语
国家	South Korea
收录类别	SCI-E
WOS记录号	WOS:000512482700031
WOS关键词	REDOX ; SALINITY ; PLANTS ; HOMEOSTASIS ; RESPONSES ; REDUCTASE ; DROUGHT ; PROTEIN ; GROWTH ; NPR1
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315343
作者单位	1.Gyeongsang Natl Univ, Div Appl Life Sci BK21 Plus, Jinju 52828, South Korea; 2.Konkuk Univ, Inst Glocal Dis Control, Seoul 05029, South Korea; 3.Rural Dev Adm, Dept Agr Biotechnol, Natl Inst Agr Sci, Jeonju 54874, South Korea; 4.Gyeongsang Natl Univ, PMBBRC, IALS, Jinju 52828, South Korea; 5.Gyeongsang Natl Univ, RILS, Jinju 52828, South Korea; 6.Konkuk Univ, Dept Biomed Sci & Engn, Seoul 05029, South Korea
推荐引用方式 GB/T 7714	Ji, Myung Geun,Park, Hee Jin,Cha, Joon-Yung,et al. Expression of Arabidopsis thaliana Thioredoxin-h2 in Brassica napus enhances antioxidant defenses and improves salt tolerance[J],2020,147:313-321.
APA	Ji, Myung Geun.,Park, Hee Jin.,Cha, Joon-Yung.,Kim, Jin A..,Shin, Gyeong-Im.,...&Kim, Woe-Yeon.(2020).Expression of Arabidopsis thaliana Thioredoxin-h2 in Brassica napus enhances antioxidant defenses and improves salt tolerance.PLANT PHYSIOLOGY AND BIOCHEMISTRY,147,313-321.
MLA	Ji, Myung Geun,et al."Expression of Arabidopsis thaliana Thioredoxin-h2 in Brassica napus enhances antioxidant defenses and improves salt tolerance".PLANT PHYSIOLOGY AND BIOCHEMISTRY 147(2020):313-321.