干旱区生态环境知识资源中心(Arid): Dehydration-Induced DnaK2 Chaperone Is Involved in PSII Repair of a Desiccation-Tolerant Cyanobacterium(1)

Arid

DOI	10.1104/pp.19.01149
	Dehydration-Induced DnaK2 Chaperone Is Involved in PSII Repair of a Desiccation-Tolerant Cyanobacterium(1)
	Xu, Hai-Feng 1,2; Dai, Guo-Zheng 1,2; Ye, De-Min 1,2; Shang, Jin-Long 1,2; Song, Wei-Yu 1,2; Shi, Huazhong 1,2,3; Qiu, Bao-Sheng 1,2
通讯作者	Qiu, Bao-Sheng
来源期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
EISSN	1532-2548
出版年	2020
卷号	182 期号:4 页码:1991-2005
英文摘要	Maintaining the structural integrity of the photosynthetic apparatus during dehydration is critical for effective recovery of photosynthetic activity upon rehydration in a variety of desiccation-tolerant plants, but the underlying molecular mechanism is largely unclear. The subaerial cyanobacterium Nostoc flagelliforme can survive extreme dehydration conditions and quickly recovers its photosynthetic activity upon rehydration. In this study, we found that the expression of the molecular chaperone NfDnaK2 was substantially induced by dehydration, and NfDnaK2 proteins were primarily localized in the thylakoid membrane. NfDnaJ9 was identified to be the cochaperone partner of NfDnaK2, and their encoding genes shared similar transcriptional responses to dehydration. NfDnaJ9 interacted with the NfFtsH2 protease involved in the degradation of damaged D1 protein. Heterologous expression of NfdnaK2 enhanced PSII repair and drought tolerance in transgenic Nostoc sp. PCC 7120. Furthermore, the nitrate reduction (NarL)/nitrogen fixation (FixJ) family transcription factors response regulator (NfRre1) and photosynthetic electron transport-dependent regulator (NfPedR) were identified as putative positive regulators capable of binding to the promoter region of NfdnaK2 and they may mediate dehydration-induced expression of NfdnaK2 in N. flagelliforme. Our findings provide novel insights into the molecular mechanism of desiccation tolerance in some xerotolerant microorganisms, which could facilitate future synthetic approaches to the creation of extremophiles in microorganisms and plants. The drought tolerance of the cyanobacterium Nostoc flagelliforme is enhanced by a dehydration-induced chaperone pair that is involved in photosystem II repair under stress conditions.
类型	Article
语种	英语
国家	Peoples R China ; USA
开放获取类型	Green Published
收录类别	SCI-E
WOS记录号	WOS:000526827500051
WOS关键词	NOSTOC-FLAGELLIFORME CYANOPHYCEAE ; SP STRAIN PCC-6803 ; BLUE-GREEN-ALGA ; PHOTOSYSTEM-II ; GENE-EXPRESSION ; TERRESTRIAL CYANOBACTERIUM ; IN-VITRO ; PHOTOSYNTHETIC RECOVERY ; DESERT CYANOBACTERIA ; ARABIDOPSIS-THALIANA
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315342
作者单位	1.Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China; 2.Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China; 3.Texas Tech Univ, Dept Chem & Biochem, Lubbock, TX 79409 USA
推荐引用方式 GB/T 7714	Xu, Hai-Feng,Dai, Guo-Zheng,Ye, De-Min,et al. Dehydration-Induced DnaK2 Chaperone Is Involved in PSII Repair of a Desiccation-Tolerant Cyanobacterium(1)[J],2020,182(4):1991-2005.
APA	Xu, Hai-Feng.,Dai, Guo-Zheng.,Ye, De-Min.,Shang, Jin-Long.,Song, Wei-Yu.,...&Qiu, Bao-Sheng.(2020).Dehydration-Induced DnaK2 Chaperone Is Involved in PSII Repair of a Desiccation-Tolerant Cyanobacterium(1).PLANT PHYSIOLOGY,182(4),1991-2005.
MLA	Xu, Hai-Feng,et al."Dehydration-Induced DnaK2 Chaperone Is Involved in PSII Repair of a Desiccation-Tolerant Cyanobacterium(1)".PLANT PHYSIOLOGY 182.4(2020):1991-2005.