Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s12298-019-00733-w |
Callus culture and plantlet regeneration in date palm (Phoneix dactylifera L.): an important horticultural cash crop for arid and semi-arid horticulture | |
Rathore, Mangal S.; Patel, Prakash R.; Siddiqui, Shahrukh A. | |
通讯作者 | Rathore, Mangal S. |
来源期刊 | PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
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ISSN | 0971-5894 |
EISSN | 0974-0430 |
出版年 | 2020 |
卷号 | 26期号:2页码:391-398 |
英文摘要 | Phoneix dactylifera L. commonly called date palm is a highly valuable horticultural cash crop for arid and semi-arid regions. The availability of offshoots and their survival during plantation are major concern. Being dioecious tree, seed propagation in date palm do not produce true-to-type offspring and tissue culture propagation is the only viable option to supply quality-planting propagules. Hereby, we report callus culture and plantlet regeneration in female date palm using in vitro-derived adventitious shoot bud tissues as explants. Explants (89.33 +/- 2.67%) produced callus culture on 0.8% agar-gelled Murashige and Skoog's basal medium containing 100.0 mg l(-1) each polyvinylpyrrolidone, ascorbic acid and glutamine, 50.0 mg l(-1) each citric acid, adenine sulphate and l-arginine as additives, 0.1% activated charcoal (AC), 100 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) and 3.0 mg l(-1) 2-isopentenyladenine (2-iP). Callus culture were amplified on medium containing 3.0 mg l(-1) 2-iP along with 50 mg l(-1) 2,4-D for 2 passages and 10 mg l(-1) 2,4-D for 2 passages. Cultures grew moderately, organized and subsequently regenerated into shoot bud like structures during gradual transfer from medium containing higher concentration of 2,4-D to lower concentration. Plantlets were developed by sub-culturing of differentiated buds on (1) hormone free medium supplied with 10.0% sucrose and (2) medium containing 100.0 mg l(-1) each ascorbic acid and glutamine, 50.0 mg l(-1) each citric acid, adenine sulphate and l-arginine as additives, 1.0 mg l(-1) each 6-benzylaminopurine, kinetin, 2-iP and alpha-naphthaleneacetic acid. Plantlets were developed on medium containing 0.1% AC, 1.0 mg l(-1) each indole-3-acetic acid and indole-3-butyric acid. Rooted plantlets were soil-transplanted and acclimatized through gradual exposure from in vitro to in vivo conditions. Simple adoption, higher culture regeneration and simultaneous production of rooted plantlets in a cyclic manner render the protocol useful for mass scale propagation of elite genotype of female date palm. |
英文关键词 | Dioecious Female plant Genetic improvement Indirect regeneration Organogenesis and tissue culture |
类型 | Article |
语种 | 英语 |
国家 | India |
开放获取类型 | Green Published |
收录类别 | SCI-E |
WOS记录号 | WOS:000515154600017 |
WOS关键词 | EMBRYOGENIC SUSPENSION-CULTURES ; JATROPHA-CURCAS L. ; ALOE-VERA L. ; SOMATIC EMBRYOGENESIS ; SHOOT REGENERATION ; GENETIC STABILITY ; GENOTYPE ; RAPD ; PROLIFERATION ; PROPAGATION |
WOS类目 | Plant Sciences |
WOS研究方向 | Plant Sciences |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/315316 |
作者单位 | Marine Chemicals Res Inst, Council Sci,Biotechnol Div,Appl Phycol,CSIR, Cent Salt,CSMCRI,Ind Res, G.B. Marg, Bhavnagar 364002, Gujarat, India |
推荐引用方式 GB/T 7714 | Rathore, Mangal S.,Patel, Prakash R.,Siddiqui, Shahrukh A.. Callus culture and plantlet regeneration in date palm (Phoneix dactylifera L.): an important horticultural cash crop for arid and semi-arid horticulture[J],2020,26(2):391-398. |
APA | Rathore, Mangal S.,Patel, Prakash R.,&Siddiqui, Shahrukh A..(2020).Callus culture and plantlet regeneration in date palm (Phoneix dactylifera L.): an important horticultural cash crop for arid and semi-arid horticulture.PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS,26(2),391-398. |
MLA | Rathore, Mangal S.,et al."Callus culture and plantlet regeneration in date palm (Phoneix dactylifera L.): an important horticultural cash crop for arid and semi-arid horticulture".PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS 26.2(2020):391-398. |
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