干旱区生态环境知识资源中心(Arid): Callus culture and plantlet regeneration in date palm (Phoneix dactylifera L.): an important horticultural cash crop for arid and semi-arid horticulture

Arid

DOI	10.1007/s12298-019-00733-w
	Callus culture and plantlet regeneration in date palm (Phoneix dactylifera L.): an important horticultural cash crop for arid and semi-arid horticulture
	Rathore, Mangal S.; Patel, Prakash R.; Siddiqui, Shahrukh A.
通讯作者	Rathore, Mangal S.
来源期刊	PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
ISSN	0971-5894
EISSN	0974-0430
出版年	2020
卷号	26 期号:2 页码:391-398
英文摘要	Phoneix dactylifera L. commonly called date palm is a highly valuable horticultural cash crop for arid and semi-arid regions. The availability of offshoots and their survival during plantation are major concern. Being dioecious tree, seed propagation in date palm do not produce true-to-type offspring and tissue culture propagation is the only viable option to supply quality-planting propagules. Hereby, we report callus culture and plantlet regeneration in female date palm using in vitro-derived adventitious shoot bud tissues as explants. Explants (89.33 +/- 2.67%) produced callus culture on 0.8% agar-gelled Murashige and Skoog's basal medium containing 100.0 mg l(-1) each polyvinylpyrrolidone, ascorbic acid and glutamine, 50.0 mg l(-1) each citric acid, adenine sulphate and l-arginine as additives, 0.1% activated charcoal (AC), 100 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) and 3.0 mg l(-1) 2-isopentenyladenine (2-iP). Callus culture were amplified on medium containing 3.0 mg l(-1) 2-iP along with 50 mg l(-1) 2,4-D for 2 passages and 10 mg l(-1) 2,4-D for 2 passages. Cultures grew moderately, organized and subsequently regenerated into shoot bud like structures during gradual transfer from medium containing higher concentration of 2,4-D to lower concentration. Plantlets were developed by sub-culturing of differentiated buds on (1) hormone free medium supplied with 10.0% sucrose and (2) medium containing 100.0 mg l(-1) each ascorbic acid and glutamine, 50.0 mg l(-1) each citric acid, adenine sulphate and l-arginine as additives, 1.0 mg l(-1) each 6-benzylaminopurine, kinetin, 2-iP and alpha-naphthaleneacetic acid. Plantlets were developed on medium containing 0.1% AC, 1.0 mg l(-1) each indole-3-acetic acid and indole-3-butyric acid. Rooted plantlets were soil-transplanted and acclimatized through gradual exposure from in vitro to in vivo conditions. Simple adoption, higher culture regeneration and simultaneous production of rooted plantlets in a cyclic manner render the protocol useful for mass scale propagation of elite genotype of female date palm.
英文关键词	Dioecious Female plant Genetic improvement Indirect regeneration Organogenesis and tissue culture
类型	Article
语种	英语
国家	India
开放获取类型	Green Published
收录类别	SCI-E
WOS记录号	WOS:000515154600017
WOS关键词	EMBRYOGENIC SUSPENSION-CULTURES ; JATROPHA-CURCAS L. ; ALOE-VERA L. ; SOMATIC EMBRYOGENESIS ; SHOOT REGENERATION ; GENETIC STABILITY ; GENOTYPE ; RAPD ; PROLIFERATION ; PROPAGATION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315316
作者单位	Marine Chemicals Res Inst, Council Sci,Biotechnol Div,Appl Phycol,CSIR, Cent Salt,CSMCRI,Ind Res, G.B. Marg, Bhavnagar 364002, Gujarat, India
推荐引用方式 GB/T 7714	Rathore, Mangal S.,Patel, Prakash R.,Siddiqui, Shahrukh A.. Callus culture and plantlet regeneration in date palm (Phoneix dactylifera L.): an important horticultural cash crop for arid and semi-arid horticulture[J],2020,26(2):391-398.
APA	Rathore, Mangal S.,Patel, Prakash R.,&Siddiqui, Shahrukh A..(2020).Callus culture and plantlet regeneration in date palm (Phoneix dactylifera L.): an important horticultural cash crop for arid and semi-arid horticulture.PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS,26(2),391-398.
MLA	Rathore, Mangal S.,et al."Callus culture and plantlet regeneration in date palm (Phoneix dactylifera L.): an important horticultural cash crop for arid and semi-arid horticulture".PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS 26.2(2020):391-398.