干旱区生态环境知识资源中心(Arid): Identification and characterization of multiple dsRNases from a lepidopteran insect, the tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae)

Arid

DOI	10.1016/j.pestbp.2019.09.011
	Identification and characterization of multiple dsRNases from a lepidopteran insect, the tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae)
	Peng, Yingchuan 1,2; Wang, Kangxu 1; Zhu, Guanheng 3; Han, Qunxin 4; Chen, Jiasheng 1; Elzaki, Mohammed Esmail Abdalla 1; Sheng, Chengwang 1; Zhao, Chunqing 1; Palli, Subba Reddy 3; Han, Zhaojun 1
通讯作者	Han, Zhaojun
来源期刊	PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
ISSN	0048-3575
EISSN	1095-9939
出版年	2020
卷号	162 页码:86-95
英文摘要	RNA interference (RNAi) efficiency varies among insects. RNAi is highly efficient and systemic in coleopteran insects but quite variable and inefficient in lepidopteran insects. Degradation of double-stranded RNA (dsRNA) by double-stranded ribonucleases (dsRNases) is thought to contribute to the variability in RNAi efficiency observed among insects. One or two dsRNases involved in dsRNA digestion have been identified in a few insects. To understand the contribution of dsRNases to reduced RNAi efficiency in lepidopteran insects, we searched the transcriptome of Spodoptera litura and identified six genes coding for DNA/RNA nonspecific endonucleases. Phylogenetic analysis revealed the evolutionary expansion of dsRNase genes in insects. The mRNA levels of three midgut-specific dsRNases increased during the larval stage, and the highest dsRNA-degrading activity was detected in third-instar larvae. Proteins produced via the expression of three midgut-specific dsRNases, and the widely expressed dsRNase3, in a baculovirus system showed dsRNase activity for four out of five dsRNases tested. In addition, the increase in dsRNA-degrading activity and upregulation of dsRNase1and 2 in larvae fed on cabbage leaves suggests that the diet of S. litura can influence dsRNase expression, dsRNA stability, and thus probably RNAi efficiency. This is the first report that multiple dsRNases function together in an RNAi-recalcitrant insect. The data included in this paper suggest that multiple dsRNases coded by the S. litura genome might contribute to the lower and variable RNAi efficiency reported in this and other lepidopteran insects.
类型	Article
语种	英语
国家	Peoples R China ; USA
收录类别	SCI-E
WOS记录号	WOS:000510967200012
WOS关键词	DOUBLE-STRANDED-RNA ; NUCLEASE ACTIVITY ; DESERT LOCUST ; INTERFERENCE ; DELIVERY ; DEGRADATION ; EXPRESSION ; GENERATION ; EFFICIENCY ; EFFICACY
WOS类目	Biochemistry & Molecular Biology ; Entomology ; Physiology
WOS研究方向	Biochemistry & Molecular Biology ; Entomology ; Physiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315303
作者单位	1.Nanjing Agr Univ, Coll Plant Protect, Dept Entomol, Agr Minist Key Lab Monitoring & Management Plant, Nanjing 210095, Peoples R China; 2.Jiangxi Agr Univ, Inst Entomol, Nanchang 330045, Jiangxi, Peoples R China; 3.Univ Kentucky, Coll Agr Food & Environm, Dept Entomol, Lexington, KY 40546 USA; 4.Zhongkai Univ Agr & Engn, Dept Plant Protect, Guangzhou 510225, Peoples R China
推荐引用方式 GB/T 7714	Peng, Yingchuan,Wang, Kangxu,Zhu, Guanheng,et al. Identification and characterization of multiple dsRNases from a lepidopteran insect, the tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae)[J],2020,162:86-95.
APA	Peng, Yingchuan.,Wang, Kangxu.,Zhu, Guanheng.,Han, Qunxin.,Chen, Jiasheng.,...&Han, Zhaojun.(2020).Identification and characterization of multiple dsRNases from a lepidopteran insect, the tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae).PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,162,86-95.
MLA	Peng, Yingchuan,et al."Identification and characterization of multiple dsRNases from a lepidopteran insect, the tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae)".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 162(2020):86-95.