Knowledge Resource Center for Ecological Environment in Arid Area
| DOI | 10.7717/peerj.8686 |
| In vitro anticancer activity of methanolic extract of Granulocystopsis sp., a microalgae from an oligotrophic oasis in the Chihuahuan desert | |
| Tavares-Carreon, Faviola1; De la Torre-Zavala, Susana1; Fernando Arocha-Garza, Hector1; Souza, Valeria2; Galan-Wong, Luis J.1; Aviles-Arnaut, Hamlet1 | |
| 通讯作者 | Aviles-Arnaut, Hamlet |
| 来源期刊 | PEERJ
 |
| ISSN | 2167-8359 |
| 出版年 | 2020 |
| 卷号 | 8 |
| 英文摘要 | With the purpose of discovering new anticancer molecules that might have fewer side effects or reduce resistance to current antitumor drugs, a bioprospecting study of the microalgae of the Cuatro Cienegas Basin (CCB), an oasis in the Chihuahuan desert in Mexico was conducted. A microalgae was identified as Granulocystopsis sp. through sequencing the rbcL gene and reconstruction of a phylogenetic tree, and its anticancer activities were assessed using various in vitro assays and different cell lines of human cancers, including lung, skin melanoma, colorectal, breast and prostatic cancers, as well as a normal cell line. The values of IC50 of the microalgae methanolic extract using the MTT assay were lower than 20 mu g/ml, except that in the lung cancer line and the normal cell line. In vitro, the microalgae extract caused the loss of membrane integrity, monitored by the trypan blue exclusion test and exhibited marked inhibition of adhesion and cell proliferation in cancer cell lines, through the evaluation of the clonogenic assay. Also, typical nuclear changes of apoptotic processes were observed under the microscope, using the dual acridine orange/ethidium bromide fluorescent staining. Finally, the microalgae extract increased the activity of caspases 3 and 7 in skin melanoma, colon, breast and prostate cancer cells, in the same way as the apoptotic inductor and powerful antitumoral drug, doxorubicin. This study shows the anticancer activity from Granulocystopsis sp., a microalgae isolated from the CCB. |
| 英文关键词 | Microalgae extract Cuatro cienegas Anticancer activity Apoptosis |
| 类型 | Article |
| 语种 | 英语 |
| 国家 | Mexico |
| 开放获取类型 | Green Published, gold |
| 收录类别 | SCI-E |
| WOS记录号 | WOS:000519629900005 |
| WOS关键词 | CUATRO CIENEGAS BASIN ; FRESH-WATER MICROALGA ; BREAST-CANCER CELLS ; DUNALIELLA-SALINA ; GROWTH ; COAHUILA ; DNA ; CYTOTOXICITY ; BIODIVERSITY ; INFLAMMATION |
| WOS类目 | Multidisciplinary Sciences |
| WOS研究方向 | Science & Technology - Other Topics |
| 来源机构 | Universidad Nacional Autónoma de México |
| 资源类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/315293 |
| 作者单位 | 1.Univ Autonoma Nuevo Leon, Inst Biotecnol, Fac Ciencias Biol, San Nicolas De Los Garza, Nuevo Leon, Mexico; 2.Univ Nacl Autonoma Mexico, Inst Ecol, Dept Ecol Evolut, Coyoacan, Mexico |
| 推荐引用方式 GB/T 7714 | Tavares-Carreon, Faviola,De la Torre-Zavala, Susana,Fernando Arocha-Garza, Hector,et al. In vitro anticancer activity of methanolic extract of Granulocystopsis sp., a microalgae from an oligotrophic oasis in the Chihuahuan desert[J]. Universidad Nacional Autónoma de México,2020,8. |
| APA | Tavares-Carreon, Faviola,De la Torre-Zavala, Susana,Fernando Arocha-Garza, Hector,Souza, Valeria,Galan-Wong, Luis J.,&Aviles-Arnaut, Hamlet.(2020).In vitro anticancer activity of methanolic extract of Granulocystopsis sp., a microalgae from an oligotrophic oasis in the Chihuahuan desert.PEERJ,8. |
| MLA | Tavares-Carreon, Faviola,et al."In vitro anticancer activity of methanolic extract of Granulocystopsis sp., a microalgae from an oligotrophic oasis in the Chihuahuan desert".PEERJ 8(2020). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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