干旱区生态环境知识资源中心(Arid): Performance Analysis of Anaplasma Antibody Competitive ELISA Using the ROC Curve for Screening of Anaplasmosis in Camel Populations in Egypt

Arid

DOI	10.3390/pathogens9030165
	Performance Analysis of Anaplasma Antibody Competitive ELISA Using the ROC Curve for Screening of Anaplasmosis in Camel Populations in Egypt
	Parvizi, Omid 1; El-Adawy, Hosny 1,2; Roesler, Uwe 3; Neubauer, Heinrich 1; Mertens-Scholz, Katja 1
通讯作者	Parvizi, Omid
来源期刊	PATHOGENS
EISSN	2076-0817
出版年	2020
卷号	9 期号:3
英文摘要	Anaplasmosis is a tick-born and potential zoonotic disease caused by Anaplasma (A.) phagocytophilum, A. ovis, A. platys and A. capra. Anaplasma marginale affecting bovines and camels causing significant economic losses. Camels as an integral part of the socio-economic lifestyle of nomads in semi-arid to arid ecosystems are prone to suffer from subclinical Anaplasma infections. This study aimed to determine the performance and adaptation of commercial competitive Anaplasma ELISA (cELISA) as a tool for screening the seroprevalence of anaplasmosis whitin the camel populations in Egypt. This study was based on the serological investigation of 437 camel sera collected between 2015 and 2016 during a Q fever prevalence study in Egypt using commercially available cELISA for the detection of antibodies specific for Anaplasma in bovine serum. The receiver operating characteristic (ROC) curve, an analysis method for optimizing cutoff values in cELISAs, was used to estimate the sensitivity and specificity using 76 true as serological positive (n = 7) and negative (n = 60) for Anaplasma antibodies. ROC curve analysis was done for 7 true positive and 60 true negative bovine samples and 7 true positive and 29 true negative camel samples serum. Real time PCR and/or conventional PCR was applied to confirm Anaplasma spp. specific-DNA in camel serum as an indication of a true positive and true negative for ROC analysis. Chi square analysis was performed to estimate the association between risk factors and anaplasmosis in camels. The cutoff value was determined as 0.42 (p value <= 0.001). Data simulation with randomly generated values revealed a cutoff value of 0.417 (p <= 0.001) with resulting 58.1% Se and 97.8% Sp. Seven true positive and 29 true negative camel serum samples was confirmed by PCR. Using the estimated cut off, the seroprevalence in the Nile Valley and Delta and the Eastern Desert domain was 47.4% and 46.4%, respectively. The potential risk factors as domains and origin of animals were less significantly associated with the prevalence of anaplasmosis (domains: chi(2) = 41.8, p value <= 0.001 and origin: chi(2) = 42.56, p value <= 0.001). Raising awareness especially for veterinarians and animal owners will significantly contribute to the best understanding of anaplasmosis in camels in Egypt. Alternative (in silico) validation techniques and preliminary prevalence studies are mandatory towards the control of neglected anaplasmosis in the camel population.
英文关键词	anaplasmosis camel ROC curve real time PCR cELISA
类型	Article
语种	英语
国家	Germany ; Egypt
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000524306100053
WOS关键词	ENZYME-LINKED-IMMUNOSORBENT ; MOLECULAR-DETECTION ; MARGINALE ; EHRLICHIA ; PHAGOCYTOPHILUM ; VALIDATION ; INFECTION ; DIAGNOSIS ; AGENTS ; DOGS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315284
作者单位	1.Friedrich Loeffler Inst, Inst Bacterial Infect & Zoonoses, Fed Res Inst Anim Hlth, D-07743 Jena, Germany; 2.Kafrelsheikh Univ, Fac Vet Med, Kafr Al Sheikh 33516, Egypt; 3.Free Univ Berlin, Inst Anim Hyg & Environm Hlth, D-14163 Berlin, Germany
推荐引用方式 GB/T 7714	Parvizi, Omid,El-Adawy, Hosny,Roesler, Uwe,et al. Performance Analysis of Anaplasma Antibody Competitive ELISA Using the ROC Curve for Screening of Anaplasmosis in Camel Populations in Egypt[J],2020,9(3).
APA	Parvizi, Omid,El-Adawy, Hosny,Roesler, Uwe,Neubauer, Heinrich,&Mertens-Scholz, Katja.(2020).Performance Analysis of Anaplasma Antibody Competitive ELISA Using the ROC Curve for Screening of Anaplasmosis in Camel Populations in Egypt.PATHOGENS,9(3).
MLA	Parvizi, Omid,et al."Performance Analysis of Anaplasma Antibody Competitive ELISA Using the ROC Curve for Screening of Anaplasmosis in Camel Populations in Egypt".PATHOGENS 9.3(2020).