干旱区生态环境知识资源中心(Arid): Chidamide Inhibits Glioma Cells by Increasing Oxidative Stress via the miRNA-338-5p Regulation of Hedgehog Signaling

Arid

DOI	10.1155/2020/7126976
	Chidamide Inhibits Glioma Cells by Increasing Oxidative Stress via the miRNA-338-5p Regulation of Hedgehog Signaling
	Zhou, Haixia 1; Han, Liang 2; Wang, Han 3; Wei, Jun 4; Guo, Zhigang 5; Li, Zhaohui 5
通讯作者	Guo, Zhigang ; Li, Zhaohui
来源期刊	OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
ISSN	1942-0900
EISSN	1942-0994
出版年	2020
卷号	2020
英文摘要	Objective. Chidamide has a broad spectrum of antitumor activity but its function on glioma remains unknown. The increase of reactive oxygen species (ROS) and reactive nitrogen species (RNS) may control glioma risk by promoting its apoptosis and necrosis. Hedgehog pathway is crucial to glioma cell proliferation and controls ROS production. We aimed to explore the effects of chidamide on the levels of miR-338-5p (glioma cell inhibitor), which may regulate Hedgehog signaling, resulting in the changes of RNS. Materials and Methods. Migration and invasion activities of glioma cells were measured by using the Transwell chamber assay. The expression levels of Sonic Hedgehog (Shh), Indian Hedgehog (Ihh), Desert Hedgehog (Dhh), miR-338-5p, and related molecules were detected by using real-time PCR (RT-PCR) and or Western Blot in U87 and HS683 glioma cells. The effects of chidamide on these molecules were measured by using the miR-338-5p inhibitor or mimics in U87 and HS683 glioma cell lines. ROS and RNS were measured by DCF DA and DAF-FM DA fluorescence. Biomarkers of oxidative stress were measured by using a corresponding kit. Apoptosis and necrosis rates were measured by using flow cytometry. Results. Chidamide inhibited the growth rate, migration, and invasion of human malignant glioma cells and increased the level of miR-338-5p. miR-338-5p inhibitor or mimics increased or inhibited the growth rate of U87 and HS683 glioma cells. Chidamide inhibited the levels of Shh, Ihh, migration protein E-cadherin, and invading protein MMP-2. The increase in the level of Shh and Ihh led to the reduction in the ROS and RNS levels. miR-338-5p inhibitor or mimics also showed a promoting or inhibitory function for the levels of Shh and Ihh. Furthermore, miR-338-5p mimics and inhibitor inhibited or promoted the migration and invasion of the glioma cells (P<0.05). Evaluated levels of miR-338-5p increased oxidative stress level and apoptosis and necrosis rate by regulating the levels of biomarkers of oxidative stress (P<0.05). Conclusion. Chidamide inhibits glioma cells by increasing oxidative stress via the miRNA-338-5p regulation of Hedgehog signaling. Chidamide may be a potential drug in the prevention of glioma development.
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000522265500001
WOS关键词	HISTONE DEACETYLASE INHIBITOR ; EPITHELIAL-MESENCHYMAL TRANSITION ; GASTRIC-CANCER CELLS ; MESSENGER-RNA LEVELS ; DOWN-REGULATION ; INVASION ; PATHWAY ; PROLIFERATION ; MIGRATION ; APOPTOSIS
WOS类目	Cell Biology
WOS研究方向	Cell Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315262
作者单位	1.Jilin Univ, China Japan Union Hosp, VIP Unit, Changchun 130033, Peoples R China; 2.Jilin Univ, China Japan Union Hosp, Dept Pathol, Changchun 130033, Peoples R China; 3.Chinese Med Univ, Affiliated Hosp, Dept Clin Lab, Changchun 130021, Peoples R China; 4.Jilin Univ, China Japan Union Hosp, Surg Inst, Changchun 130033, Peoples R China; 5.Jilin Univ, China Japan Union Hosp, Dept Neurosurg, Changchun 130033, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Haixia,Han, Liang,Wang, Han,et al. Chidamide Inhibits Glioma Cells by Increasing Oxidative Stress via the miRNA-338-5p Regulation of Hedgehog Signaling[J],2020,2020.
APA	Zhou, Haixia,Han, Liang,Wang, Han,Wei, Jun,Guo, Zhigang,&Li, Zhaohui.(2020).Chidamide Inhibits Glioma Cells by Increasing Oxidative Stress via the miRNA-338-5p Regulation of Hedgehog Signaling.OXIDATIVE MEDICINE AND CELLULAR LONGEVITY,2020.
MLA	Zhou, Haixia,et al."Chidamide Inhibits Glioma Cells by Increasing Oxidative Stress via the miRNA-338-5p Regulation of Hedgehog Signaling".OXIDATIVE MEDICINE AND CELLULAR LONGEVITY 2020(2020).