干旱区生态环境知识资源中心(Arid): Regulatory Noncoding Small RNAs Are Diverse and Abundant in an Extremophilic Microbial Community

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DOI	10.1128/mSystems.00584-19
	Regulatory Noncoding Small RNAs Are Diverse and Abundant in an Extremophilic Microbial Community
	Gelsinger, Diego R.; Uritskiy, Gherman; Reddy, Rahul; Munn, Adam; Farney, Katie; DiRuggiero, Jocelyne
通讯作者	DiRuggiero, Jocelyne
来源期刊	MSYSTEMS
ISSN	2379-5077
出版年	2020
卷号	5 期号:1
英文摘要	Regulatory small RNAs (sRNAs) play large-scale and essential roles in many cellular processes across all domains of life. Microbial sRNAs have been extensively studied in model organisms, but very little is known about the dynamics of sRNA synthesis and their roles in the natural environment. In this study, we discovered hundreds of intergenic (itsRNAs) and antisense (asRNAs) sRNAs expressed in an extremophilic microbial community inhabiting halite nodules (salt rocks) in the Atacama Desert. For this, we built SnapT, a new sRNA annotation pipeline that can be applied to any microbial community. We found asRNAs with expression levels negatively correlated with that of their overlapping putative target and itsRNAs that were conserved and significantly differentially expressed between 2 sampling time points. We demonstrated that we could perform target prediction and correlate expression levels between sRNAs and predicted target mRNAs at the community level. Functions of putative mRNA targets reflected the environmental challenges members of the halite communities were subjected to, including osmotic adjustments to a major rain event and competition for nutrients. IMPORTANCE Microorganisms in the natural world are found in communities, communicating and interacting with each other; therefore, it is essential that microbial regulatory mechanisms, such as gene regulation affected by small RNAs (sRNAs), be investigated at the community level. This work demonstrates that metatranscriptomic field experiments can link environmental variation with changes in RNA pools and have the potential to provide new insights into environmental sensing and responses in natural microbial communities through noncoding RNA-mediated gene regulation.
英文关键词	RNA extremophiles gene regulation metagenomics metatranscriptomics microbial communities microbiome noncoding
类型	Article
语种	英语
国家	USA
开放获取类型	gold, Green Published, Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000518855000014
WOS关键词	BACTERIAL SMALL RNAS ; PREDICTION
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315198
作者单位	Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA
推荐引用方式 GB/T 7714	Gelsinger, Diego R.,Uritskiy, Gherman,Reddy, Rahul,et al. Regulatory Noncoding Small RNAs Are Diverse and Abundant in an Extremophilic Microbial Community[J],2020,5(1).
APA	Gelsinger, Diego R.,Uritskiy, Gherman,Reddy, Rahul,Munn, Adam,Farney, Katie,&DiRuggiero, Jocelyne.(2020).Regulatory Noncoding Small RNAs Are Diverse and Abundant in an Extremophilic Microbial Community.MSYSTEMS,5(1).
MLA	Gelsinger, Diego R.,et al."Regulatory Noncoding Small RNAs Are Diverse and Abundant in an Extremophilic Microbial Community".MSYSTEMS 5.1(2020).