干旱区生态环境知识资源中心(Arid): Morphological description and phylogenetic assessment of 28S rRNA for Thelandros chalcidiae sp. nov. from Chalcides ocellatus

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DOI	10.1007/s11033-020-05412-8
	Morphological description and phylogenetic assessment of 28S rRNA for Thelandros chalcidiae sp. nov. from Chalcides ocellatus
	Abdel-Ghaffar, Fathy 1; Varjabedian, Kohar Garo 1; Al Quraishy, Saleh 2; Abdel-Gaber, Rewaida 1,2; Fol, Mona 1; Talal, Noha 1
通讯作者	Abdel-Gaber, Rewaida
来源期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
EISSN	1573-4978
出版年	2020
卷号	47 期号:5 页码:3705-3718
英文摘要	Thelandros is a genus of oxyurid nematodes which parasitize both omnivorous and herbivorous hosts. Thelandros chalcidiae sp. nov. is a new taxa described from the large intestine of the ocellated skink, Chalcides ocellatus, from the South Sinai Desert in Egypt. The recovered parasite species was examined at both morphological and molecular levels in order to determine the exact taxonomic position within Pharyngodonidae family. The current pharyngodonid species is characterized by a mouth opening bounded by three bilobed lips; male worms are characterized by the presence of two pairs of cloacal papillae (pre- and adcloacal) and one single caudal papillae (postcloacal) and caudal alae in an auricular form and females with post-equatorial vulva, amphidelphic ovary in which anterior ovary extended to level of excretory pore and posterior ovary extended posteriorly up anal opening. The recovered nematodes were compared with other known species from different hosts and it was found to be morphologically different from them. Molecular characterization based on the partial 28S rRNA nuclear ribosomal gene sequence showed sequence identities >= 83.15% with taxa under family Pharyngodonidae, 74.84-87.37% with Oxyuridae, 80.54% with Heteroxynematidae, and 75.98-77.72% with Thelastomatidea. Phylogenetic analysis showed that parasite sequence in conjunction with existing data facilitate placement of this species within Oxyurida. The present species is deeply embedded in genus Thelandros with close relationships to previously described Thelandros sp. and T. galloti in same taxon. This study highlighted importance of combining morphological and genetic data with taxonomy in pharyngodonid species.
英文关键词	Thelandros species Reptiles Morphology Phylogenetic assessment 28S rRNA nuclear ribosomal gene Egypt
类型	Article
语种	英语
国家	Egypt ; Saudi Arabia
收录类别	SCI-E
WOS记录号	WOS:000525261600001
WOS关键词	N. SP NEMATODA ; MOLECULAR CHARACTERIZATION ; PHARYNGODONIDAE NEMATODA ; OXYURIDA TRAVASSOSINEMATIDAE ; SQUAMATA AGAMIDAE ; EGYPTIAN LIZARD ; PARAPHARYNGODON ; OXYUROIDEA ; HELMINTHS ; EVOLUTION
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
来源机构	King Saud University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315187
作者单位	1.Cairo Univ, Fac Sci, Zool Dept, Cairo, Egypt; 2.King Saud Univ, Coll Sci, Zool Dept, Riyadh, Saudi Arabia
推荐引用方式 GB/T 7714	Abdel-Ghaffar, Fathy,Varjabedian, Kohar Garo,Al Quraishy, Saleh,et al. Morphological description and phylogenetic assessment of 28S rRNA for Thelandros chalcidiae sp. nov. from Chalcides ocellatus[J]. King Saud University,2020,47(5):3705-3718.
APA	Abdel-Ghaffar, Fathy,Varjabedian, Kohar Garo,Al Quraishy, Saleh,Abdel-Gaber, Rewaida,Fol, Mona,&Talal, Noha.(2020).Morphological description and phylogenetic assessment of 28S rRNA for Thelandros chalcidiae sp. nov. from Chalcides ocellatus.MOLECULAR BIOLOGY REPORTS,47(5),3705-3718.
MLA	Abdel-Ghaffar, Fathy,et al."Morphological description and phylogenetic assessment of 28S rRNA for Thelandros chalcidiae sp. nov. from Chalcides ocellatus".MOLECULAR BIOLOGY REPORTS 47.5(2020):3705-3718.