干旱区生态环境知识资源中心(Arid): Understanding the Response of Nitrifying Communities to Disturbance in the McMurdo Dry Valleys, Antarctica

Arid

DOI	10.3390/microorganisms8030404
	Understanding the Response of Nitrifying Communities to Disturbance in the McMurdo Dry Valleys, Antarctica
	Monteiro, Maria 1,2; Baptista, Mafalda S.2,3,6; Seneca, Joana 3; Torgo, Luis 4,5,6; Lee, Charles K.1,2; Cary, S. Craig 1,2; Magalhaes, Catarina 1,3,6,7
通讯作者	Magalhaes, Catarina
来源期刊	MICROORGANISMS
EISSN	2076-2607
出版年	2020
卷号	8 期号:3
英文摘要	Polar ecosystems are generally limited in nitrogen (N) nutrients, and the patchy availability of N is partly determined by biological pathways, such as nitrification, which are carried out by distinctive prokaryotic functional groups. The activity and diversity of microorganisms are generally strongly influenced by environmental conditions. However, we know little of the attributes that control the distribution and activity of specific microbial functional groups, such as nitrifiers, in extreme cold environments and how they may respond to change. To ascertain relationships between soil geochemistry and the ecology of nitrifying microbial communities, we carried out a laboratory-based manipulative experiment to test the selective effect of key geochemical variables on the activity and abundance of ammonia-oxidizing communities in soils from the McMurdo Dry Valleys of Antarctica. We hypothesized that nitrifying communities, adapted to different environmental conditions within the Dry Valleys, will have distinct responses when submitted to similar geochemical disturbances. In order to test this hypothesis, soils from two geographically distant and geochemically divergent locations, Miers and Beacon Valleys, were incubated over 2 months under increased conductivity, ammonia concentration, copper concentration, and organic matter content. Amplicon sequencing of the 16S rRNA gene and transcripts allowed comparison of the response of ammonia-oxidizing Archaea (AOA) and ammonia-oxidizing Bacteria (AOB) to each treatment over time. This approach was combined with measurements of (NH4+)-N-15 oxidation rates using N-15 isotopic additions. Our results showed a higher potential for nitrification in Miers Valley, where environmental conditions are milder relative to Beacon Valley. AOA exhibited better adaptability to geochemical changes compared to AOB, particularly to the increase in copper and conductivity. AOA were also the only nitrifying group found in Beacon Valley soils. This laboratorial manipulative experiment provided new knowledge on how nitrifying groups respond to changes on key geochemical variables of Antarctic desert soils, and we believe these results offer new insights on the dynamics of N cycling in these ecosystems.
英文关键词	McMurdo Dry Valleys nitrogen cycle 16S rRNA gene 16S rRNA transcripts nitrifying communities ammonia oxidation manipulative experiment
类型	Article
语种	英语
国家	New Zealand ; Portugal ; Canada
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000525952700092
WOS关键词	AMMONIA-OXIDIZERS ; RIBOSOMAL-RNA ; MICROBIAL RESPONSES ; NITRATE REDUCTION ; NITROGEN ; NITRIFICATION ; DIVERSITY ; SOIL ; SEQUENCES ; ARCHAEAL
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315177
作者单位	1.Univ Waikato, Sch Sci, Hamilton 3240, New Zealand; 2.Univ Waikato, Int Ctr Terr Antarctic Res, Hamilton 3240, New Zealand; 3.Univ Porto, Interdisciplinary Ctr Marine & Environm Res CIIMA, P-4450208 Matosinhos, Portugal; 4.LIAAD INESC Porto LA, R Ceuta 118-6, P-4050190 Porto, Portugal; 5.Dalhousie Univ, Fac Comp Sci, 6050 Univ Av, Halifax, NS N3H 1W5, Canada; 6.Univ Porto, Fac Sci, P-4169007 Porto, Portugal; 7.Dalhousie Univ, Ocean Frontier Inst, Halifax, NS B3H 4R2, Canada
推荐引用方式 GB/T 7714	Monteiro, Maria,Baptista, Mafalda S.,Seneca, Joana,et al. Understanding the Response of Nitrifying Communities to Disturbance in the McMurdo Dry Valleys, Antarctica[J],2020,8(3).
APA	Monteiro, Maria.,Baptista, Mafalda S..,Seneca, Joana.,Torgo, Luis.,Lee, Charles K..,...&Magalhaes, Catarina.(2020).Understanding the Response of Nitrifying Communities to Disturbance in the McMurdo Dry Valleys, Antarctica.MICROORGANISMS,8(3).
MLA	Monteiro, Maria,et al."Understanding the Response of Nitrifying Communities to Disturbance in the McMurdo Dry Valleys, Antarctica".MICROORGANISMS 8.3(2020).