干旱区生态环境知识资源中心(Arid): Induction of drought tolerance in tomato upon the application of ACC deaminase producing plant growth promoting rhizobacterium Bacillus subtilis Rhizo SF 48

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DOI	10.1016/j.micres.2020.126422
	Induction of drought tolerance in tomato upon the application of ACC deaminase producing plant growth promoting rhizobacterium Bacillus subtilis Rhizo SF 48
	Gowtham, H. G.1; Singh, Brijesh S.1; Murali, M.2; Shilpa, N.1; Prasad, Melvin 1; Aiyaz, Mohammed 1; Amruthesh, K. N.2; Niranjana, S. R.1
通讯作者	Amruthesh, K. N. ; Niranjana, S. R.
来源期刊	MICROBIOLOGICAL RESEARCH
ISSN	0944-5013
出版年	2020
卷号	234
英文摘要	A total of ten 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing PGPR isolates were selected and evaluated for the induction of drought stress tolerance in tomato. Among the selected PGPR, maximum seed (laboratory) and plant growth promotion (greenhouse) was observed in tomato seeds bacterized with Bacillus subtilis Rhizo SF 48. The genomic study confirmed the presence of ACC deaminase gene in Rhizo SF 48 and the obtained sequence was deposited to the NCBI database with the Accession No. MK652706. The tomato plants grown upon treatment with Rhizo SF 48 significantly enhanced plant growth even after exposing to different levels of drought stress as compared to stress induced control plants. About 7.5% and 38% increase in RWC were observed in Rhizo SF 48 treated tomato plants grown under well-watered and stress conditions (S4) compared to their control plants, respectively. An increase of 0.76, 0.23 and 0.78 fold in proline, SOD and APX activity and a decrease of 0.3 fold in MDA and H2O2 contents were observed in Rhizo SF 48 treated plants compared to control plants grown under S4 conditions. The histo-chemical studies showed lower accumulations of H2O2 and superoxide anion in the leaves of Rhizo SF 48 treated plants under drought stress, which was in confirmation with the quantification results of H2O2 and SOD. The qRT-PCR studies on drought (Le25) and ethylene responsive factor (SlERF84) marker genes showed that a significant decrease of 0.75 and 0.81 folds, respectively in Le25 and SlERF84 accumulation was observed in Rhizo SF 48 treated plants compared to untreated plants grown under S4 conditions. From the results, it can be attributed that ACC deaminase producing Rhizo SF 48 was able to protect tomato plants against oxidative damage caused due to drought stress and enhanced plant growth promotion. It can be concluded that ACC deaminase producing Rhizo SF 48 can serve as a useful bio-inoculant for sustainable tomato production in arid and semi-arid regions with water deficit.
英文关键词	ACC deaminase Antioxidants Bacillus subtilis Drought Plant growth promoting rhizobacteria Solanum lycopersicum L.
类型	Article
语种	英语
国家	India
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:000526039000002
WOS关键词	ABIOTIC STRESS ; HYDROGEN-PEROXIDE ; OXIDATIVE STRESS ; ANTIOXIDANT ACTIVITY ; WATER-STRESS ; ETHYLENE ; BACTERIA ; ACID ; LYCOPERSICON ; EXPRESSION
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315169
作者单位	1.Univ Mysore, Dept Studies Biotechnol, Mysuru 570006, Karnataka, India; 2.Univ Mysore, Dept Studies Bot, Appl Plant Pathol Lab, Mysuru 570006, Karnataka, India
推荐引用方式 GB/T 7714	Gowtham, H. G.,Singh, Brijesh S.,Murali, M.,et al. Induction of drought tolerance in tomato upon the application of ACC deaminase producing plant growth promoting rhizobacterium Bacillus subtilis Rhizo SF 48[J],2020,234.
APA	Gowtham, H. G..,Singh, Brijesh S..,Murali, M..,Shilpa, N..,Prasad, Melvin.,...&Niranjana, S. R..(2020).Induction of drought tolerance in tomato upon the application of ACC deaminase producing plant growth promoting rhizobacterium Bacillus subtilis Rhizo SF 48.MICROBIOLOGICAL RESEARCH,234.
MLA	Gowtham, H. G.,et al."Induction of drought tolerance in tomato upon the application of ACC deaminase producing plant growth promoting rhizobacterium Bacillus subtilis Rhizo SF 48".MICROBIOLOGICAL RESEARCH 234(2020).