干旱区生态环境知识资源中心(Arid): The grapevine NaE sodium exclusion locus encodes sodium transporters with diverse transport properties and localisation

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DOI	10.1016/j.jplph.2020.153113
	The grapevine NaE sodium exclusion locus encodes sodium transporters with diverse transport properties and localisation
	Wu, Yue 1,2; Henderson, Sam W.1,2,5; Wege, Stefanie 1,2; Zheng, Fei 1,2; Walker, Amanda R.3; Walker, Rob R.3; Gilliham, Matthew 1,2,4
通讯作者	Gilliham, Matthew
来源期刊	JOURNAL OF PLANT PHYSIOLOGY
ISSN	0176-1617
EISSN	1618-1328
出版年	2020
卷号	246
英文摘要	Grapevine (Vitis vinifera L.) is a valuable crop for human consumption and wine production, and is prone to suffering from salinity stress in arid regions or when exposed to low quality irrigation water. A previous study identified a quantitative trait locus (QTL) NaE, containing six High-affinity Potassium Transporter 1 genes, that was associated with shoot Na+ exclusion in grapevine. While HKT1;1 was predicted to be the most likely gene within this QTL to encode for this important salinity tolerance sub-trait, four other HKTs within the QTL remained uncharacterised; VviHKT1;2 encodes a truncated transcript unlikely to form a functional transporter. In this study, two allelic variants for each of VviHKT1;6, VviHKT1;7 and VviHKT1;8 from the heterozygous grapevine variety Cabernet Sauvignon were functionally characterised. Using the Xenopus laevis oocyte heterologous expression system, as well as transient expression in tobacco leaves, we found that the VviHKT1;6 and VviHKT1;7 alleles encoded plasma membrane localised proteins that facilitated significant non-rectifying Na+ transport. Conversely, proteins encoded by the VviHKT1;8 alleles were inwardly-rectifying, weak Na+ transporters that localised to intracellular organelles. Mining of previous RNA-seq gene expression data suggested that VviHKT1;6-8 are weakly expressed in grapevine roots, flower buds, and seeds under normal conditions and different nutrient regimes. We propose that VviHKT1;6 and VviHKT1;7 are likely to have a less significant role in grapevine leaf Na+ exclusion than VviHKT1;1, and that VviHKT1;8 is involved in endomembrane Na+ transport.
英文关键词	Grapevine Vitis vinifera Salt exclusion HKT Sodium transporter Electrophysiology
类型	Article
语种	英语
国家	Australia
收录类别	SCI-E
WOS记录号	WOS:000519607200013
WOS关键词	L. CV SULTANA ; HKT TRANSPORTERS ; SALT TOLERANCE ; SALINITY TOLERANCE ; POTASSIUM UPTAKE ; PLANT ; ARABIDOPSIS ; WHEAT ; EXPRESSION ; CHLORIDE
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	Commonwealth Scientific and Industrial Research Organisation
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315056
作者单位	1.Univ Adelaide, ARC Ctr Excellence Plant Energy Biol, Sch Agr Food & Wine, Glen Osmond, SA 5064, Australia; 2.Univ Adelaide, Waite Res Inst, Glen Osmond, SA 5064, Australia; 3.CSIRO Agr & Food, Locked Bag 2, Glen Osmond, SA 5064, Australia; 4.Univ Adelaide, ARC Ind Transformat Training Ctr Innovat Wine Pro, Sch Agr Food & Wine, Glen Osmond, SA 5064, Australia; 5.Univ Adelaide, Adelaide Med Sch, Adelaide, SA 5005, Australia
推荐引用方式 GB/T 7714	Wu, Yue,Henderson, Sam W.,Wege, Stefanie,et al. The grapevine NaE sodium exclusion locus encodes sodium transporters with diverse transport properties and localisation[J]. Commonwealth Scientific and Industrial Research Organisation,2020,246.
APA	Wu, Yue.,Henderson, Sam W..,Wege, Stefanie.,Zheng, Fei.,Walker, Amanda R..,...&Gilliham, Matthew.(2020).The grapevine NaE sodium exclusion locus encodes sodium transporters with diverse transport properties and localisation.JOURNAL OF PLANT PHYSIOLOGY,246.
MLA	Wu, Yue,et al."The grapevine NaE sodium exclusion locus encodes sodium transporters with diverse transport properties and localisation".JOURNAL OF PLANT PHYSIOLOGY 246(2020).