Knowledge Resource Center for Ecological Environment in Arid Area
| DOI | 10.1007/s11060-019-03384-9 |
| Short non-coding RNA sequencing of glioblastoma extracellular vesicles | |
| de Mooij, Tristan1; Peterson, Timothy E.1; Evans, Jared2; McCutcheon, Brandon1; Parney, Ian F.1,3 | |
| 通讯作者 | Parney, Ian F. |
| 来源期刊 | JOURNAL OF NEURO-ONCOLOGY
 |
| ISSN | 0167-594X |
| EISSN | 1573-7373 |
| 出版年 | 2020 |
| 卷号 | 146期号:2页码:253-263 |
| 英文摘要 | Introduction Like all nucleated cells, glioblastoma (GBM) cells shed small membrane-encapsulated particles called extracellular vesicles (EVs). EVs can transfer oncogenic components and promote tumor growth by transferring short non-coding RNAs, altering target cell gene expression. Furthermore, GBM-derived EVs can be detected in blood and have potential to serve as liquid biopsies. Methods EVs were harvested from culture supernatants from human GBM cell lines, purified via sequential centrifugation, and quantified by nanoparticle tracking. RNA was isolated and short non-coding RNA was sequenced. Data was analyzed via the OASIS-2.0 platform using HG38. MirTarBase and MirDB interrogated validated/predicted miRNA-gene interactions respectively. Results Many short non-coding RNA's were identified within GBM EV's. In keeping with earlier reports utilizing GBM EV micro-RNA (miRNA) arrays, these included abundant micro-RNA's including miR-21. However, RNA sequencing revealed a total of 712 non-coding RNA sequences most of which have not been associated with GBM EV's previously. These included many RNA species (piRNA, snoRNA, snRNA, rRNA and yRNAs) in addition to miRNA's. miR-21-5p, let-7b-5p, miR-3182, miR-4448, let-7i-5p constituted highest overall expression. Top genes targeted by non-coding RNA's were highly conserved and specific for cell cycle, PI3K/Akt signaling, p53 and Glioma curated KEGG pathways. Conclusions Next generation short non-coding RNA sequencing on GBM EV's validates findings from earlier studies using miRNA arrays but also demonstrates expression of many additional non-coding RNA sequences and classes previously unassociated with GBM. This may yield important insights into pathophysiology, point to new therapeutic targets, and help develop new biomarkers for disease burden and treatment response. |
| 英文关键词 | Glioblastoma Extracellular vesicles Exosomes microRNA Short non-coding RNA |
| 类型 | Article |
| 语种 | 英语 |
| 国家 | USA |
| 收录类别 | SCI-E |
| WOS记录号 | WOS:000519279400001 |
| WOS关键词 | EXOSOMES ; SERUM |
| WOS类目 | Oncology ; Clinical Neurology |
| WOS研究方向 | Oncology ; Neurosciences & Neurology |
| 资源类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/315040 |
| 作者单位 | 1.Mayo Clin, Dept Neurol Surg, 200 First St SW, Rochester, MN 55901 USA; 2.Mayo Clin, Dept Biomed Informat, Rochester, MN USA; 3.Mayo Clin, Dept Immunol, Rochester, MN 55901 USA |
| 推荐引用方式 GB/T 7714 | de Mooij, Tristan,Peterson, Timothy E.,Evans, Jared,et al. Short non-coding RNA sequencing of glioblastoma extracellular vesicles[J],2020,146(2):253-263. |
| APA | de Mooij, Tristan,Peterson, Timothy E.,Evans, Jared,McCutcheon, Brandon,&Parney, Ian F..(2020).Short non-coding RNA sequencing of glioblastoma extracellular vesicles.JOURNAL OF NEURO-ONCOLOGY,146(2),253-263. |
| MLA | de Mooij, Tristan,et al."Short non-coding RNA sequencing of glioblastoma extracellular vesicles".JOURNAL OF NEURO-ONCOLOGY 146.2(2020):253-263. |
| 条目包含的文件 | 条目无相关文件。 | |||||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
