干旱区生态环境知识资源中心(Arid): Histochemical localisation of carbonic anhydrase in the digestive tract and salivary glands of the house cricket, Acheta domesticus

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DOI	10.3920/JIFF2019.0033
	Histochemical localisation of carbonic anhydrase in the digestive tract and salivary glands of the house cricket, Acheta domesticus
	Thorsson, E.1; Jansson, A.2; Vaga, M.2; Holm, L.2
通讯作者	Thorsson, E.
来源期刊	JOURNAL OF INSECTS AS FOOD AND FEED
EISSN	2352-4588
出版年	2020
卷号	6 期号:2 页码:191-198
英文摘要	The house cricket (Acheta domesticus) is one of several cricket species with great potential to be farmed as a sustainable protein source. In order to succeed in large-scale cricket farming, knowledge of cricket digestion is essential. The digestive tract morphology of A. domesticus is well documented, but knowledge of the salivary glands is lacking. In the digestive tract of insects, the carbonic anhydrase (CA) enzyme family is believed to contribute to the luminal pH gradient. Presence of CA in the digestive tract of A. domesticus has been reported, but not the cellular localisation. This study examined the digestive tract of A. domesticus, including salivary glands, and the cellular localisation and activity of CA in fed or starved (48 h) males and females. Tissues were collected from third-generation offspring of wild A. domesticus captured in Sweden and the histology of the salivary glands and the cellular localisation of CA in the digestive tract of A. domesticus were determined, to our knowledge for the first time. The salivary glands resembled those of grasshoppers and locusts, and we suggest the two main cell types present to be parietal and zymogenic cells. Histochemical analysis revealed that CA activity was localised in midgut epithelium, both main cell types of salivary gland, and muscle along the entire digestive tract. These findings support the suggestion that CA contributes to digestive tract luminal pH gradient, by driving acidic secretions from the salivary glands and alkaline secretions from the midgut. Starvation resulted in significantly reduced body size and weight, but neither starvation nor sex had any effect on CA activity or localisation.
英文关键词	histology gastrointestinal tract pH enzyme histochemistry
类型	Article
语种	英语
国家	Sweden
开放获取类型	Green Published, Bronze
收录类别	SCI-E
WOS记录号	WOS:000524982700011
WOS关键词	FINE-STRUCTURE ; DESERT LOCUST ; ORTHOPTERA ; SECRETION ; INHIBITION ; COCKROACH ; ENZYMES ; MIDGUT ; MUSCLE ; ACID
WOS类目	Agriculture, Multidisciplinary ; Entomology ; Food Science & Technology
WOS研究方向	Agriculture ; Entomology ; Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/315018
作者单位	1.Swedish Univ Agr Sci SLU, Dept Biomed Sci & Vet Publ Hlth, Div Pathol, POB 7028, S-75007 Uppsala, Sweden; 2.Swedish Univ Agr Sci SLU, Dept Anat Physiol & Biochem, POB 7011, S-75007 Uppsala, Sweden
推荐引用方式 GB/T 7714	Thorsson, E.,Jansson, A.,Vaga, M.,et al. Histochemical localisation of carbonic anhydrase in the digestive tract and salivary glands of the house cricket, Acheta domesticus[J],2020,6(2):191-198.
APA	Thorsson, E.,Jansson, A.,Vaga, M.,&Holm, L..(2020).Histochemical localisation of carbonic anhydrase in the digestive tract and salivary glands of the house cricket, Acheta domesticus.JOURNAL OF INSECTS AS FOOD AND FEED,6(2),191-198.
MLA	Thorsson, E.,et al."Histochemical localisation of carbonic anhydrase in the digestive tract and salivary glands of the house cricket, Acheta domesticus".JOURNAL OF INSECTS AS FOOD AND FEED 6.2(2020):191-198.