干旱区生态环境知识资源中心(Arid): Three Chemosensory Proteins Involved in Chemoreception of Oedaleus asiaticus (Orthopera: Acridoidea)

Arid

DOI	10.1007/s10886-019-01138-5
	Three Chemosensory Proteins Involved in Chemoreception of Oedaleus asiaticus (Orthopera: Acridoidea)
	Zhou, Yuan-Tao 1,2; Li, Ling 1; Zhou, Xiao-Rong 1; Tan, Yao 1; Pang, Bao-Ping 1
通讯作者	Pang, Bao-Ping
来源期刊	JOURNAL OF CHEMICAL ECOLOGY
ISSN	0098-0331
EISSN	1573-1561
出版年	2020
卷号	46 期号:2 页码:138-149
英文摘要	Chemosensory proteins (CSPs) are thought to play roles in the insect olfactory system by binding and carrying hydrophobic odorants across the aqueous sensillar lymph. The band-winged grasshopper, Oedaleus asiaticus Bei-Bienko, is one of the most important grasshopper pests in northern China, but there is little information about its olfactory system. In order to investigate the olfactory functions of CSPs in this pest, three CSP genes (OasiCSP4, OasiCSP11 and OasiCSP12) were expressed in Escherichia coli, and the binding affinities of the three recombinant CSP proteins were measured for 16 volatiles from the host plant (Stipa krylovii), fecal material and body of live adult O. asiaticus using fluorescence competitive binding assays. To further verify their olfactory functions, RNA interference (RNAi) and electrophysiological recording were conducted. The three recombinant proteins displayed different degrees of binding to various volatiles in ligand-binding assays, with OasiCSP12 having higher binding affinities for more volatiles than OasiCSP4 and OasiCSP11. OasiCSP12 exhibited strong binding affinities (Ki < 20 mu M) for five host plant volatiles and one volatile from the live body of adult O. asiaticus. The transcript levels of the three OasiCSP genes were significantly lower after silencing the individual genes by RNAi, which in turn reduced the EAG responses in adults of both sexes to most tested compounds. Our study indicates that these three OasiCSPs are involved in the detection of volatile semiochemicals, and may play important roles in finding host plants and in aggregation in O. asiaticus.
英文关键词	Oedaleus asiaticus Chemosensory protein Semiochemicals RNA interference Fluorescence competitive binding assay Electroantennogram
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000518187300003
WOS关键词	AGGREGATION PHEROMONE SYSTEM ; GREGARIOUS DESERT LOCUST ; BINDING-PROTEINS ; ODORANT-BINDING ; EXPRESSION ; ORTHOPTERA ; GRASSHOPPER ; GENES ; IDENTIFICATION ; LOCALIZATION
WOS类目	Biochemistry & Molecular Biology ; Ecology
WOS研究方向	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314911
作者单位	1.Inner Mongolia Agr Univ, Res Ctr Grassland Entomol, Hohhot 010020, Peoples R China; 2.Qinghai Univ, Coll Agr & Anim Husb, Xining 810016, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Yuan-Tao,Li, Ling,Zhou, Xiao-Rong,et al. Three Chemosensory Proteins Involved in Chemoreception of Oedaleus asiaticus (Orthopera: Acridoidea)[J],2020,46(2):138-149.
APA	Zhou, Yuan-Tao,Li, Ling,Zhou, Xiao-Rong,Tan, Yao,&Pang, Bao-Ping.(2020).Three Chemosensory Proteins Involved in Chemoreception of Oedaleus asiaticus (Orthopera: Acridoidea).JOURNAL OF CHEMICAL ECOLOGY,46(2),138-149.
MLA	Zhou, Yuan-Tao,et al."Three Chemosensory Proteins Involved in Chemoreception of Oedaleus asiaticus (Orthopera: Acridoidea)".JOURNAL OF CHEMICAL ECOLOGY 46.2(2020):138-149.