干旱区生态环境知识资源中心(Arid): Novel Amino Acid Assembly in the Silk Tubes of Arid-Adapted Segestriid Spiders

Arid

DOI	10.1007/s10886-019-01127-8
	Novel Amino Acid Assembly in the Silk Tubes of Arid-Adapted Segestriid Spiders
	Conti, Erminia 1; Dattilo, Sandro 2; Scamporrino, Andrea 2; Costa, Giovanni 1; Samperi, Filippo 2
通讯作者	Conti, Erminia
来源期刊	JOURNAL OF CHEMICAL ECOLOGY
ISSN	0098-0331
EISSN	1573-1561
出版年	2020
卷号	46 期号:1 页码:48-62
英文摘要	We investigated in different sites inside or outside the Namib Desert the amino acids composition of the protein material forming the tube silk of Ariadna spiders. These spiders belong to the primitive Segestriidae family and spend their life inside vertical silk burrows dug within the sandy and gravelly soil of arid areas. The silks, previously purified by solubilization in hexafluoroisopropanol, were subjected to partial or total acid hydrolysis. Partial hydrolyzed samples, analyzed by mass spectrometry (matrix assisted laser desorption/ionization and electrospray), led to relevant information on the amino acid sequences in the proteins. The free amino acids formed by complete hydrolysis were derivatized with the Marfey's reagent and characterized by electrospray mass spectrometry. The reconstruction of the amino acids highlights a homogeneous plan in the chemical structure of all the analyzed silks. Eight amino acids constituting the primary structure of the proteins were identified. Alanine and glycine are the most abundant ones, with a prevalence of alanine, constituting together at least 61% of the chemical composition of the protein material, differently from what occurs in known spidroins. High percentages of proline, serine and threonine and low percentages of leucine complete the peculiarity of these proteins. The purified silks were also characterized by Fourier-transform Infrared Spectroscopy and their thermal properties were investigated by differential scanning calorimetry. The comparison of the silk tubes among the various Namibian populations, carried out through a multivariate statistical analysis, shows significant differences in their amino acid assembly possibly due to habitat features.
英文关键词	Amino acids Ecological adaptations Namibia Segestriidae Spider silk
类型	Article
语种	英语
国家	Italy
收录类别	SCI-E
WOS记录号	WOS:000518387500005
WOS关键词	MECHANICAL-PROPERTIES ; MASS-SPECTROMETRY ; AMPULLATE SILKS ; COB WEAVER ; PROTEINS ; ARANEAE ; FIBERS ; HYDROLYSIS ; PROPERTY ; DRAGLINE
WOS类目	Biochemistry & Molecular Biology ; Ecology
WOS研究方向	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314910
作者单位	1.Univ Catania, Dipartimento Sci Biol Geol & Ambientali, I-95124 Catania, Italy; 2.CNR, IPCB, I-95126 Catania, Italy
推荐引用方式 GB/T 7714	Conti, Erminia,Dattilo, Sandro,Scamporrino, Andrea,et al. Novel Amino Acid Assembly in the Silk Tubes of Arid-Adapted Segestriid Spiders[J],2020,46(1):48-62.
APA	Conti, Erminia,Dattilo, Sandro,Scamporrino, Andrea,Costa, Giovanni,&Samperi, Filippo.(2020).Novel Amino Acid Assembly in the Silk Tubes of Arid-Adapted Segestriid Spiders.JOURNAL OF CHEMICAL ECOLOGY,46(1),48-62.
MLA	Conti, Erminia,et al."Novel Amino Acid Assembly in the Silk Tubes of Arid-Adapted Segestriid Spiders".JOURNAL OF CHEMICAL ECOLOGY 46.1(2020):48-62.