干旱区生态环境知识资源中心(Arid): Conversion and Stability of New Metabolites of Paralytic Shellfish Toxins under Different Temperature and pH Conditions

Arid

DOI	10.1021/acs.jafc.9b07063
	Conversion and Stability of New Metabolites of Paralytic Shellfish Toxins under Different Temperature and pH Conditions
	Che, Yijia; Ding, Ling; Qiu, Jiangbing; Ji, Ying; Li, Aifeng
通讯作者	Li, Aifeng
来源期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
EISSN	1520-5118
出版年	2020
卷号	68 期号:5 页码:1427-1435
英文摘要	A number of new C-11 hydroxyl metabolites (so-called M-toxins) of paralytic shellfish toxins (PSTs) have been discovered in contaminated shellfish, and trace amounts have also been detected in some strains of PST-producing microalgae. To investigate the chemical conversion and stability of M-toxins, mussel extracts were purified with solid-phase extraction cartridges (Oasis HLB) and Biogel P-2 resin columns and four partially purified M-toxin fractions were stored at different temperatures (-20, 4, and 20 degrees C) and pH values (3, 4, and 5). The concentrations and profiles of M-toxins in these fractions were analyzed using liquid chromatography coupled with tandem mass spectrometry for 27 weeks. Results further confirmed the chemical conversion pathway M1 -> M3 -> M5 and determined for the first time two new transformation pathways: M2 -> M4 -> M6 and neosaxitoxin (NEO) -> M10. The half-lives of M1, M2, M4, and M10 were calculated using a first-order degradation kinetics model, which indicated that the degradation of all M-toxins was dependent upon the temperature and pH, increasing with rising temperature and pH. In comparison to M4 and M10, Ml was more sensitive to the temperature, followed by M2. Results suggest that M-toxins should be maintained at a low temperature (-20 degrees C) and low pH (3) for their prolonged storage. M-toxins were less stable than all of the common analogues of PSTs, which may be beneficial for shellfish to achieve rapid detoxification through transformation of PSTs to M-toxins. These new findings are of significance because they enable further understanding of the metabolism of PSTs and their detoxification mechanisms in contaminated shellfish.
英文关键词	paralytic shellfish toxins (PSTs) metabolites chemical conversion stability liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000512222600027
WOS关键词	IN-VITRO TRANSFORMATION ; THERMAL-DEGRADATION ; POISONING TOXINS ; MYTILUS-GALLOPROVINCIALIS ; SAXITOXIN ANALOGS ; CRASSOSTREA-GIGAS ; PSP TOXINS ; ACCUMULATION ; PURIFICATION ; PROFILES
WOS类目	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314850
作者单位	Ocean Univ China, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Che, Yijia,Ding, Ling,Qiu, Jiangbing,et al. Conversion and Stability of New Metabolites of Paralytic Shellfish Toxins under Different Temperature and pH Conditions[J],2020,68(5):1427-1435.
APA	Che, Yijia,Ding, Ling,Qiu, Jiangbing,Ji, Ying,&Li, Aifeng.(2020).Conversion and Stability of New Metabolites of Paralytic Shellfish Toxins under Different Temperature and pH Conditions.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,68(5),1427-1435.
MLA	Che, Yijia,et al."Conversion and Stability of New Metabolites of Paralytic Shellfish Toxins under Different Temperature and pH Conditions".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 68.5(2020):1427-1435.