干旱区生态环境知识资源中心(Arid): Bartonella kosoyi sp. nov. and Bartonella krasnovii sp. nov., two novel species closely related to the zoonotic Bartonella elizabethae, isolated from black rats and wild desert rodent-fleas

Arid

DOI	10.1099/ijsem.0.003952
	Bartonella kosoyi sp. nov. and Bartonella krasnovii sp. nov., two novel species closely related to the zoonotic Bartonella elizabethae, isolated from black rats and wild desert rodent-fleas
	Gutierrez, Ricardo 1; Shalit, Tali 2; Markus, Barak 2; Yuan, Congli 3; Nachum-Biala, Yaarit 1; Elad, Daniel 4; Harrus, Shimon 1
通讯作者	Harrus, Shimon
来源期刊	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN	1466-5026
EISSN	1466-5034
出版年	2020
卷号	70 期号:3 页码:1656-1665
英文摘要	The genus Bartonella (Family: Bartonellaceae; Order: Rhizobiales; Class: Alphaproteobacteria) comprises facultative intracellular Gram-negative, haemotropic, slow-growing, vector-borne bacteria. Wild rodents and their fleas harbor a great diversity of species and strains of the genus Bartonella, including several zoonotic ones. This genetic diversity coupled with a fastidious nature of the organism results in a taxonomic challenge that has led to a massive collection of uncharacterized strains. Here, we report the genomic and phenotypic characterization of two strains, members of the genus Bartonella (namely Tel Aviv and OE 1-1), isolated from Rattus rattus rats and Synosternus cleopatrae fleas, respectively. Scanning electron microscopy revealed rod-shaped bacteria with polar pill, lengths ranging from 1.0 to 2.0 mu m and widths ranging from 0.3 to 0.6 mu m. OE 1-1 and Tel Aviv strains contained one single chromosome of 2.16 and 2.23 Mbp and one plasmid of 29.0 and 41.5 Kbp, with average DNA G+C contents of 38.16 and 38.47mo1%, respectively. These strains presented an average nucleotide identity (ANI) of 89.9%. Bartonella elizabethae was found to be the closest phylogenetic relative of both strains (ANI=90.9 93.6%). The major fatty acids identified in both strains were C-18:1 omega 7c, C-18:0 and C-16:0. They differ from B. elizabethae in their C-17:0 and C-15:0 compositions. Both strains are strictly capnophilic and their biochemical profiles resembled those of species of the genus Bartonella with validly published names, whereas differences in arylamidase activities partially assisted in their speciation. Genomic and phenotypic differences demonstrate that OE 1-1 and Tel Aviv strains represent novel individual species, closely related to B. elizabethae, for which we propose the names Bartonella kosoyi sp. nov. and Bartonella krasnovii sp. nov.
英文关键词	rodents rats fleas diversity
类型	Article
语种	英语
国家	Israel ; Peoples R China
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:000522929700030
WOS关键词	SMALL MAMMALS ; GENETIC-HETEROGENEITY ; COMB-NOV ; PREVALENCE ; STRAINS ; BLOOD ; SPP. ; ROCHALIMAEA ; TRIBOCORUM ; PROPOSALS
WOS类目	Microbiology
WOS研究方向	Microbiology
来源机构	Hebrew University of Jerusalem
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314798
作者单位	1.Hebrew Univ Jerusalem, Koret Sch Vet Med, IL-7610000 Rehovot, Israel; 2.Weizmann Inst Sci, Nancy & Stephen Grand Israel Natl Ctr Personalize, IL-7610000 Rehovot, Israel; 3.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai, Peoples R China; 4.Kimron Vet Inst, IL-50250 Bet Dagan, Israel
推荐引用方式 GB/T 7714	Gutierrez, Ricardo,Shalit, Tali,Markus, Barak,et al. Bartonella kosoyi sp. nov. and Bartonella krasnovii sp. nov., two novel species closely related to the zoonotic Bartonella elizabethae, isolated from black rats and wild desert rodent-fleas[J]. Hebrew University of Jerusalem,2020,70(3):1656-1665.
APA	Gutierrez, Ricardo.,Shalit, Tali.,Markus, Barak.,Yuan, Congli.,Nachum-Biala, Yaarit.,...&Harrus, Shimon.(2020).Bartonella kosoyi sp. nov. and Bartonella krasnovii sp. nov., two novel species closely related to the zoonotic Bartonella elizabethae, isolated from black rats and wild desert rodent-fleas.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,70(3),1656-1665.
MLA	Gutierrez, Ricardo,et al."Bartonella kosoyi sp. nov. and Bartonella krasnovii sp. nov., two novel species closely related to the zoonotic Bartonella elizabethae, isolated from black rats and wild desert rodent-fleas".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 70.3(2020):1656-1665.