干旱区生态环境知识资源中心(Arid): Molecular identification of Taenia hydatigena and Mesocestoides species based on copro-DNA analysis of wild carnivores in Mongolia

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DOI	10.1016/j.ijppaw.2019.12.004
	Molecular identification of Taenia hydatigena and Mesocestoides species based on copro-DNA analysis of wild carnivores in Mongolia
	Ulziijargal, Gelegbalsan 1; Yeruult, Chultemsuren 2; Khulan, Janchiv 3; Gantsetseg, Choijilsuren 4; Wandra, Toni 5; Yamasaki, Hiroshi 6; Narankhajid, Myadagsuren 7
通讯作者	Narankhajid, Myadagsuren
来源期刊	INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE
ISSN	2213-2244
出版年	2020
卷号	11 页码:72-82
英文摘要	Cyclophyllidean tapeworms obligatorily parasitize numerous mammalian species, including herbivores, domestic animals and humans, of which, the genera Taenia and Mesocestoides are well characterized. However, little is known about these parasitic infections in wild animals. This study aims to investigate the prevalence and distribution of Taenia sp. and Mesocestoides sp. in wild carnivores in Mongolia by identifying tapeworm species based on mtDNA analysis. The field survey was carried out in 2012-2013 in 19 provinces located in different ecological regions. A total of 405 fecal samples from wild carnivores were collected. Specific DNA markers in fecal samples was detected via copro-DNA analysis and tapeworm species were identified by DNA sequencing. From 27.7% (112/405) of samples, cox1 and 12S rRNA genes of tapeworms were amplified. Further, Taenia hydatigena (50.0%, 56/112) and two Mesocestoides species, including Mesocestoides sp.-1 (36.6%, 41/112) and Mesocestoides sp.-2 (13.4%, 15/112) were identified by DNA sequencing. The prevalence of T. hydatigena was 19.9% (27/136), 13.8% (23/167), 4.8% (3/62), and 7.5% (3/40) in wolves, red foxes, corsac foxes, and snow leopards, respectively. The prevalence of Mesocestoides sp.-1 was 14.7% (20/136), 9% (15/167), 9.7% (6/62) in wolves, red foxes, and corsac foxes, while the prevalence of Mesocestoides sp.-2 was 4.4% (6/136), 1.8% (3/167), 3.2% (2/62), and 10.0% (4/40) in wolves, red foxes, corsac foxes, and snow leopards, respectively. T. hydatigena was found throughout all ecological regions, while Mesocestoides sp.-1 was in the mountain taiga, forest-steppe, steppe, desert-steppe, and desert, and Mesocestoides sp.-2 in the alpine, forest-steppe, steppe, and desert-steppe ecoregions. This study revealed the prevalence and distribution of cyclophyllidean tapeworms in wild carnivores in Mongolia; while also confirming that wolves, red foxes, corsac foxes, and snow leopards serve as definitive hosts for unidentified Mesocestoides species.
英文关键词	Taenia hydatigena Mesocestoides sp. cox1 gene 12S rRNA gene Wild carnivores Mongolia
类型	Article
语种	英语
国家	Mongolia ; Indonesia ; Japan
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000523574400010
WOS关键词	FOXES VULPES-VULPES ; INFECTIOUS-DISEASES ; INTERMEDIATE HOSTS ; SHEEP ; DISCRIMINATION ; CYCLOPHYLLIDEA ; METACESTODES ; PARASITES ; PALLAS ; RECORD
WOS类目	Ecology ; Parasitology
WOS研究方向	Environmental Sciences & Ecology ; Parasitology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314734
作者单位	1.Mongolian Natl Univ Med Sci, Dept Lab, Ulaanbaatar, Mongolia; 2.Mongolian Natl Univ Med Sci, Dept Pharmacol, Ulaanbaatar, Mongolia; 3.Natl Univ Mongolia, Dept Biol, Ulaanbaatar, Mongolia; 4.Natl Ctr Zoonot Dis, Ulaanbaatar, Mongolia; 5.Sari Mutiara Indonesia Univ, Directorate Postgrad, Medan, Indonesia; 6.Natl Inst Infect Dis, Dept Parasitol, Tokyo, Japan; 7.Mongolian Natl Univ Med Sci, Dept Biol, Ulaanbaatar, Mongolia
推荐引用方式 GB/T 7714	Ulziijargal, Gelegbalsan,Yeruult, Chultemsuren,Khulan, Janchiv,et al. Molecular identification of Taenia hydatigena and Mesocestoides species based on copro-DNA analysis of wild carnivores in Mongolia[J],2020,11:72-82.
APA	Ulziijargal, Gelegbalsan.,Yeruult, Chultemsuren.,Khulan, Janchiv.,Gantsetseg, Choijilsuren.,Wandra, Toni.,...&Narankhajid, Myadagsuren.(2020).Molecular identification of Taenia hydatigena and Mesocestoides species based on copro-DNA analysis of wild carnivores in Mongolia.INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE,11,72-82.
MLA	Ulziijargal, Gelegbalsan,et al."Molecular identification of Taenia hydatigena and Mesocestoides species based on copro-DNA analysis of wild carnivores in Mongolia".INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE 11(2020):72-82.