干旱区生态环境知识资源中心(Arid): Overexpression of Ricinus communis L. malate synthase enhances seed tolerance to abiotic stress during germination

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DOI	10.1016/j.indcrop.2020.112110
	Overexpression of Ricinus communis L. malate synthase enhances seed tolerance to abiotic stress during germination
	Brito, Valdinei Carvalho 1,2; de Almeida, Catherine P.1; Barbosa, Rhaissa R.1; Carosio, Maria G. A.3; Ferreira, Antonio G.3; Fernandez, Luzimar G.4; de Castro, Renato D.4; Hilhorst, Henk 5; Ligterink, Wilco 5; Ribeiro, Paulo Roberto 1,2
通讯作者	Ribeiro, Paulo Roberto
来源期刊	INDUSTRIAL CROPS AND PRODUCTS
ISSN	0926-6690
EISSN	1872-633X
出版年	2020
卷号	145
英文摘要	Ricinus communis L. seeds can germinate at high temperatures, but further development of the seedlings is negatively affected. This mainly caused by impairment of energy-generating pathways when seeds are germinated at 35 degrees C. Ricinus communis malate synthase (RcMLS) is a key responsive gene in lipid mobilization and gluconeogenesis and as such might have a role in sustaining successful seed germination and seedling growth. Herein, we raised the question whether RcMLS might be involved in the biochemical and molecular mechanisms required for R.communis seed germination under unfavourable environmental conditions. For that, we used a robust approach that encompassed bioinformatics analysis, transgenic Arabidopsis thaliana (L.) Heynh seeds overexpressing RcMLS, along with phenotypical characterization of seed germination under abiotic stress. The phylogenetic tree revealed important evolutionary relationship amongst MIS sequences from R. commmis and from other crop species/model plants. Overexpression of RcMLS enhanced A. thaliana seed germination under high temperature and salt stress. For example, wild-type A. thaliana Columbia seeds (Col-0) showed 37 % of maximum germination at 35 degrees C, whereas A. thaliana seeds overexpressing RcMLS showed up to 71%. When salt stress was applied (75 inM NaCl), maximum germination of Col-0 seeds reached 37%, whereas for A. thaliana seeds overexpressing RcMLS it reached up to 93%. Nuclear Magnetic Resonance (NMR) and Gas Chromatography coupled to Time-Of-Flight Mass Spectrometry (GC-TOF-MS) metabolomics analysis showed a robust metabolic signature of A. thaliana seeds overexpressing RcMLS in response to abiotic stress. They accumulated high levels of Met, Be, fructose, glucose, and sucrose. Therefore, we suggested that overexpression of RcMLS has modulated the glyoxylate cycle and gluconeogenesis pathway in order to maintain cellular homeostasis under unfavorable environmental conditions. Our results provide important leads into the contribution of RcMLS to the underlying mechanism of R.communis seed germination under adverse environmental conditions. This might be helpful for breeding programs to develop more resistant R.communis cultivars which are more likely to sustain growth and high yield under the severe conditions found in and and semi-arid areas worldwide.
英文关键词	Abiotic stress Castor bean Functional characterization Temperature-responsive genes
类型	Article
语种	英语
国家	Brazil ; Netherlands
收录类别	SCI-E
WOS记录号	WOS:000518865400004
WOS关键词	GLYOXYSOMAL ENZYMES ; NUCLEOTIDE-SEQUENCE ; GLYOXYLATE SHUNT ; BIOSYNTHESIS ; COTYLEDONS ; ACCUMULATION ; INSIGHTS ; LOCALIZATION ; PURIFICATION ; TEMPERATURE
WOS类目	Agricultural Engineering ; Agronomy
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314725
作者单位	1.Univ Fed Bahia, Inst Quim, Dept Quim Organ, Metab Res Grp, Rua Barao Jeremoabo S-N, BR-40170115 Salvador, BA, Brazil; 2.Univ Estado Bahia, Dept Ciencias Exatas & Terra Campus 1, Programa Posgrad Quim Aplicada, Salvador, BA, Brazil; 3.Univ Fed Sao Carlos, Dept Quim, Lab Ressonancia Magnet Nucl, Sao Carlos, SP, Brazil; 4.Univ Fed Bahia, Dept Bioquim & Biofis, Lab Bioquim Biotecnol & Bioprod, Reitor Miguel Calmon S-N, BR-40160100 Salvador, BA, Brazil; 5.WU, Lab Plant Physiol, Wageningen Seed Lab, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands
推荐引用方式 GB/T 7714	Brito, Valdinei Carvalho,de Almeida, Catherine P.,Barbosa, Rhaissa R.,et al. Overexpression of Ricinus communis L. malate synthase enhances seed tolerance to abiotic stress during germination[J],2020,145.
APA	Brito, Valdinei Carvalho.,de Almeida, Catherine P..,Barbosa, Rhaissa R..,Carosio, Maria G. A..,Ferreira, Antonio G..,...&Ribeiro, Paulo Roberto.(2020).Overexpression of Ricinus communis L. malate synthase enhances seed tolerance to abiotic stress during germination.INDUSTRIAL CROPS AND PRODUCTS,145.
MLA	Brito, Valdinei Carvalho,et al."Overexpression of Ricinus communis L. malate synthase enhances seed tolerance to abiotic stress during germination".INDUSTRIAL CROPS AND PRODUCTS 145(2020).