干旱区生态环境知识资源中心(Arid): Transcriptome analysis reveals the molecular mechanisms of mucilage biosynthesis during Artemisia sphaerocephala seed development

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DOI	10.1016/j.indcrop.2019.111991
	Transcriptome analysis reveals the molecular mechanisms of mucilage biosynthesis during Artemisia sphaerocephala seed development
	Han, Xiaoxu; Zhang, Lijing; Miao, Xiumei; Hu, Xiaowei; Nan, Shuzhen; Fu, Hua
通讯作者	Zhang, Lijing
来源期刊	INDUSTRIAL CROPS AND PRODUCTS
ISSN	0926-6690
EISSN	1872-633X
出版年	2020
卷号	145
英文摘要	Seed mucilage is a polysaccharide with significant physiological, ecological, and economic value; however, both its biosynthesis pathway and molecular regulatory mechanisms remain unclear. Artemisia sphaerocephala is an important sand-fixed windbreak plant that is widely distributed across the deserts of Northwest China, with seeds that are rich in mucilage. For this research, seven different developmental stages of A. sphaerocephala seeds were employed as samples. The mucilage accumulation patterns were analyzed, and the differentially expressed genes (DEGs) involved in the biosynthesis of mucilage were identified via RNA sequencing. The results revealed that during the rapid accumulation period (10-30 days after flowering), the mucilage content increased from 0 to 49.42 %. Up-regulated expressed phosphoglucomutase (pgm) and uridine diphosphate glucose pyrophosphorylase (UGPase), and down-regulated expressed UDP-glucose 4-epimerase (GALE), UDP-glucose 6-dehydrogenase (UGDH), and UDP-glucose 4,6-dehydratase (RHM) led to the accumulation of UDP-glucose (UDP-Glc). Additionally, highly expressed UDP-apiose/xylose synthase (AXS) and minimally expressed UDP-arabinose 4-epimerase (UXE) led to the accumulation of UDP-xylose (UDP-Xyl). The MYB-bHLH-WD40 (MBW) complex and APETALA2 (AP2) were the primary transcription factors that regulated the biosynthesis of mucilage in A. sphaerocephala seeds. This study might assist with elucidating the molecular regulatory mechanisms of the seed mucilage biosynthesis process, while establishing a foundation for the application of mucilage associated genes in genetic breeding.
英文关键词	Artemisia sphaerocephala Seed development Mucilage biosynthesis Transcriptome sequencing
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000518865400066
WOS关键词	USITATISSIMUM L. MUCILAGE ; SALVIA-HISPANICA L. ; GLUCOSE PYROPHOSPHORYLASE ; UDP-GLYCOSYLTRANSFERASES ; SECRETORY-CELLS ; GENE-EXPRESSION ; COAT MUCILAGE ; DESERT SHRUB ; GERMINATION ; PROTEIN
WOS类目	Agricultural Engineering ; Agronomy
WOS研究方向	Agriculture
来源机构	兰州大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314724
作者单位	Lanzhou Univ, Key Lab Grassland Livestock Ind Innovat,Minist Ed, State Key Lab Grassland Agroecosyst,Engn Res Ctr, Coll Pastoral Agr Sci & Technol,Minist Agr & Rura, Lanzhou 730020, Peoples R China
推荐引用方式 GB/T 7714	Han, Xiaoxu,Zhang, Lijing,Miao, Xiumei,et al. Transcriptome analysis reveals the molecular mechanisms of mucilage biosynthesis during Artemisia sphaerocephala seed development[J]. 兰州大学,2020,145.
APA	Han, Xiaoxu,Zhang, Lijing,Miao, Xiumei,Hu, Xiaowei,Nan, Shuzhen,&Fu, Hua.(2020).Transcriptome analysis reveals the molecular mechanisms of mucilage biosynthesis during Artemisia sphaerocephala seed development.INDUSTRIAL CROPS AND PRODUCTS,145.
MLA	Han, Xiaoxu,et al."Transcriptome analysis reveals the molecular mechanisms of mucilage biosynthesis during Artemisia sphaerocephala seed development".INDUSTRIAL CROPS AND PRODUCTS 145(2020).