干旱区生态环境知识资源中心(Arid): Transcriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch

Arid

DOI	10.1186/s12983-019-0349-2
	Transcriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch
	Konopova, Barbora 1,2,3; Buchberger, Elisa 4; Crisp, Alastair 5
通讯作者	Konopova, Barbora
来源期刊	FRONTIERS IN ZOOLOGY
ISSN	1742-9994
出版年	2020
卷号	17 期号:1
英文摘要	Background Pleuropodia are limb-derived glandular organs that transiently appear on the first abdominal segment in embryos of insects from majority of orders. They are missing in the genetic model Drosophila and little is known about them. Experiments carried out on orthopteran insects 80 years ago indicated that the pleuropodia secrete a hatching enzyme that digests the serosal cuticle to enable the larva to hatch, but evidence by state-of-the-art molecular methods is missing. Results We used high-throughput RNA-sequencing to identify the genes expressed in the pleuropodia of the locust Schistocerca gregaria (Orthoptera). First, using transmission electron microscopy we studied the development of the pleuropodia during 11 stages of the locust embryogenesis. We show that the glandular cells differentiate and start secreting just before the definitive dorsal closure of the embryo and the secretion granules outside the cells become more abundant prior to hatching. Next, we generated a comprehensive embryonic reference transcriptome for the locust and used it to study genome wide gene expression across ten morphologicaly defined stages of the pleuropodia. We show that when the pleuropodia have morphological markers of functional organs and produce secretion, they are primarily enriched in transcripts associated with transport functions. They express genes encoding enzymes capable of digesting cuticular protein and chitin. These include the potent cuticulo-lytic Chitinase 5, whose transcript rises just before hatching. Unexpected finding was the enrichment in transcripts for immunity-related enzymes. This indicates that the pleuropodia are equipped with epithelial immunity similarly as barrier epithelia in postembryonic stages. Conclusions These data provide transcriptomic support for the historic hypothesis that pleuropodia produce cuticle-degrading enzymes and function in hatching. They may also have other functions, such as facilitation of embryonic immune defense. By the genes that they express the pleuropodia are specialized embryonic organs and apparently an important though neglected part of insect physiology.
英文关键词	Appendage Cuticle Ecdysone Embryo Gland Immunity Moulting fluid Orthoptera RNA-seq Schistocerca gregaria
类型	Article
语种	英语
国家	England ; Germany ; Czech Republic
开放获取类型	Green Submitted, Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000513705000001
WOS关键词	RNAI-MEDIATED KNOCKDOWN ; INSECT MOLTING FLUID ; DESERT LOCUST ; SCHISTOCERCA-GREGARIA ; MOLECULAR CHARACTERIZATION ; ECDYSTEROID BIOSYNTHESIS ; DROSOPHILA-MELANOGASTER ; CYTOCHROME-P450 ENZYME ; EXTERNAL MORPHOLOGY ; FUNCTIONAL-ANALYSIS
WOS类目	Zoology
WOS研究方向	Zoology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314564
作者单位	1.Univ Cambridge, Dept Zool, Cambridge, England; 2.Univ Gottingen, Dept Evolutionary Dev Genet, Gottingen, Germany; 3.Czech Acad Sci, Ctr Biol, Inst Entomol, Ceske Budejovice, Czech Republic; 4.Univ Gottingen, Dept Dev Biol, Gottingen, Germany; 5.MRC Lab Mol Biol, Cambridge, England
推荐引用方式 GB/T 7714	Konopova, Barbora,Buchberger, Elisa,Crisp, Alastair. Transcriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch[J],2020,17(1).
APA	Konopova, Barbora,Buchberger, Elisa,&Crisp, Alastair.(2020).Transcriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch.FRONTIERS IN ZOOLOGY,17(1).
MLA	Konopova, Barbora,et al."Transcriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch".FRONTIERS IN ZOOLOGY 17.1(2020).