干旱区生态环境知识资源中心(Arid): Haloterrigena sp. Strain SGH1, a Bacterioruberin-Rich, Perchlorate-Tolerant Halophilic Archaeon Isolated From Halite Microbial Communities, Atacama Desert, Chile

Arid

DOI	10.3389/fmicb.2020.00324
	Haloterrigena sp. Strain SGH1, a Bacterioruberin-Rich, Perchlorate-Tolerant Halophilic Archaeon Isolated From Halite Microbial Communities, Atacama Desert, Chile
	Flores, Nataly 1,2; Hoyos, Sebastian 1,2; Venegas, Mauricio 1,2; Galetovic, Alexandra 1,2; Zuniga, Lidia M.1,2; Fabrega, Francisca 1,2; Paredes, Bernardo 1,2; Salazar-Ardiles, Camila 1,2; Vilo, Claudia 1,2; Ascaso, Carmen 3; Wierzchos, Jacek 3; Souza-Egipsy, Virginia 4; Araya, Jorge E.2,5; Batista-Garcia, Ramon Alberto 6; Gomez-Silva, Benito 1,2
通讯作者	Gomez-Silva, Benito
来源期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
出版年	2020
卷号	11
英文摘要	An extreme halophilic archaeon, strain SGH1, is a novel microorganism isolated from endolithic microbial communities colonizing halites at Salar Grande, Atacama Desert, in northern Chile. Our study provides structural, biochemical, genomic, and physiological information on this new isolate living at the edge of the physical and chemical extremes at the Atacama Desert. SGH1 is a Gram-negative, red-pigmented, non-motile unicellular coccoid organism. Under the transmission electron microscope, strain SGH1 showed an abundant electro-dense material surrounding electron-lucent globular structures resembling gas vacuoles. Strain SGH1 showed a 16S rRNA gene sequence with a close phylogenetic relationship to the extreme halophilic archaea Haloterrigena turkmenica and Haloterrigena salina and has been denominated Haloterrigena sp. strain SGH1. Strain SGH1 grew at 20-40 degrees C (optimum 37 degrees C), at salinities between 15 and 30% (w/v) NaCl (optimum 25%) and growth was improved by addition of 50 mM KCl and 0.5% w/v casamino acids. Growth was severely restricted at salinities below 15% NaCl and cell lysis is avoided at a minimal 10% NaCl. Maximal concentrations of magnesium chloride and sodium or magnesium perchlorates that supported SGH1 growth were 0.5 and 0.15M, respectively. Haloterrigena sp. strain SGH1 accumulates bacterioruberin (BR), a C-50 xanthophyll, as the major carotenoid. Total carotenoids in strain SGH1 amounted to nearly 400 mu g BR per gram of dry biomass. Nearly 80% of total carotenoids accumulated as geometric isomers of BR: all-trans-BR (50%), 5-cis-BR (15%), 9-cis-BR (10%), 13-cis-BR (4%); other carotenoids were dehydrated derivatives of BR. Carotenogenesis in SGH1 was a reversible and salt-dependent process; transferring BR-rich cells grown in 25% (w/v) NaCl to 15% (w/v) NaCl medium resulted in depigmentation, and BR content was recovered after transference and growth of unpigmented cells to high salinity medium. Methanol extracts and purified BR isomers showed an 8-9-fold higher antioxidant activity than Trolox or beta-carotene. Both, plasma membrane integrity and mitochondrial membrane potential measurements under acute 18-h assays showed that purified BR isomers were non-toxic to cultured human THP-1 cells.
英文关键词	antioxidant archaea Atacama bacterioruberin Haloterrigena halites perchlorate toxicity
类型	Article
语种	英语
国家	Chile ; Spain ; Mexico
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000525175600001
WOS关键词	LIQUID WATER ; ANTIOXIDANT ACTIVITY ; RAMAN-SPECTROSCOPY ; HYPERARID CORE ; CAROTENOIDS ; LIFE ; BACTERIA ; HPLC ; IDENTIFICATION ; BIOSYNTHESIS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314551
作者单位	1.Univ Antofagasta, Biomed Dept, Lab Biochem, Antofagasta, Chile; 2.Univ Antofagasta, Ctr Biotechnol & Bioengn, Antofagasta, Chile; 3.Spanish Natl Res Council, Dept Biogeochem & Microbial Ecol, Natl Museum Nat Sci, Madrid, Spain; 4.Spanish Natl Res Council, Inst Mat Struct, Dept Macromol Phys, Madrid, Spain; 5.Univ Antofagasta, Dept Med Technol, Lab Mol Parasitol, Antofagasta, Chile; 6.Univ Autonoma Estado Morelos, Ctr Invest Dinam Celular, Inst Invest Ciencias Basicas & Aplicadas, Cuernavaca, Morelos, Mexico
推荐引用方式 GB/T 7714	Flores, Nataly,Hoyos, Sebastian,Venegas, Mauricio,et al. Haloterrigena sp. Strain SGH1, a Bacterioruberin-Rich, Perchlorate-Tolerant Halophilic Archaeon Isolated From Halite Microbial Communities, Atacama Desert, Chile[J],2020,11.
APA	Flores, Nataly.,Hoyos, Sebastian.,Venegas, Mauricio.,Galetovic, Alexandra.,Zuniga, Lidia M..,...&Gomez-Silva, Benito.(2020).Haloterrigena sp. Strain SGH1, a Bacterioruberin-Rich, Perchlorate-Tolerant Halophilic Archaeon Isolated From Halite Microbial Communities, Atacama Desert, Chile.FRONTIERS IN MICROBIOLOGY,11.
MLA	Flores, Nataly,et al."Haloterrigena sp. Strain SGH1, a Bacterioruberin-Rich, Perchlorate-Tolerant Halophilic Archaeon Isolated From Halite Microbial Communities, Atacama Desert, Chile".FRONTIERS IN MICROBIOLOGY 11(2020).