干旱区生态环境知识资源中心(Arid): Diversity and Antimicrobial Potential of Cultivable Endophytic Actinobacteria Associated With the Medicinal Plant Thymus roseus

Arid

DOI	10.3389/fmicb.2020.00191
	Diversity and Antimicrobial Potential of Cultivable Endophytic Actinobacteria Associated With the Medicinal Plant Thymus roseus
	Musa, Zulpiya 1,2; Ma, Jinbiao 1; Egamberdieva, Dilfuza 1,3; Mohamad, Osama Abdalla Abdelshafy 1,4; Abaydulla, Gulsumay 5; Liu, Yonghong 1; Li, Wen-Jun 1,6,7; Li, Li 1
通讯作者	Mohamad, Osama Abdalla Abdelshafy ; Li, Wen-Jun ; Li, Li
来源期刊	FRONTIERS IN MICROBIOLOGY
EISSN	1664-302X
出版年	2020
卷号	11
英文摘要	We report for the first time the isolation of endophytic actinobacteria associated with wild populations of the Chinese medicinal herb Thymus roseus Schipcz obtained from the arid land in Ili and Tacheng of the Xinjiang Province, China. Strains were isolated by special pretreatment of plant tissues and identified based on their 16S rRNA gene sequences, and their antimicrobial activities in vitro were evaluated. A total of 126 endophytic actinobacteria belonging to two classes, eight orders, 14 families, and 24 genera were isolated from different organs at the Ili and Tacheng sites. In addition, the diversity of culturable endophytic actinobacteria genera was higher at Tacheng site (n = 71, 56.35%) than the Ili site (n = 55, 43.65%). A neighbor-joining tree of 126 isolated actinobacteria showing the phylogenetic relationships based on 16S rRNA gene sequences and the genus Streptomyces was the most dominant isolate. The number of endophytic actinobacteria genera obtained from root tissues (n = 54, 42.86%) was higher compared to stem (n = 35, 27.78%) and leaf tissue (n = 37, 29.36%). Among 126 endophytic actinobacteria, 54 strains were antagonistic against at least one or more indicator organisms in vitro. Notably, most strains of Streptomyces proved antagonistic activities. For example, strain T4SB028, namely Streptomyces polyantibioticus, showed the highest inhibition ratio reached 67.06, 64.20, and 70.55% against Alternaria solani, Valsa malicola, and Valsa mali, respectively. The results demonstrate that about 30.95%, 23.01% of the tested endophytic actinobacteria were capable of producing siderophores and chitinase, respectively. Additionally, the results of the amplification of biosynthetic genes polyketide synthetase (PKS-I) and non-ribosomal peptide synthetase (NRPS) indicated that at least one antibiotic biosynthetic gene was detected in 27 (50%) of the tested strains. Our result emphasizes that the endophytic actinobacteria communities are different based on the plant tissues and the geographical environment of the sampled area. Thus, we conclude that T. roseus Schipcz. provided a rich source of endophytic actinobacteria that exhibited a broad-spectrum antimicrobial agent.
英文关键词	endophytic actinobacteria Thymus roseus Schipcz diversity antimicrobial activity medicinal plant environmental microbiology
类型	Article
语种	英语
国家	Peoples R China ; Uzbekistan ; Egypt
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000525687200001
WOS关键词	BIOLOGICAL-CONTROL ; STREPTOMYCES SP ; SP NOV. ; GROWTH ; ROOT ; IDENTIFICATION ; BACTERIA ; BIOCONTROL ; PATHOGEN ; CUCUMBER
WOS类目	Microbiology
WOS研究方向	Microbiology
来源机构	中国科学院新疆生态与地理研究所 ; 新疆大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314550
作者单位	1.Xinjiang Inst Ecol & Geog, CAS Key Lab Biogeog & Bioresource Arid Land, Urumqi, Peoples R China; 2.Coll Kashgar Vocat Technol, Dept Med, Kashgar, Peoples R China; 3.Natl Univ Uzbekistan, Fac Biol, Tashkent, Uzbekistan; 4.Arish Univ, Fac Environm Agr Sci, Dept Environm Protect, Arish, Egypt; 5.Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Lab Resources Microbiol, Urumqi, Peoples R China; 6.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou, Peoples R China; 7.Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Plant Resources, Guangzhou, Peoples R China
推荐引用方式 GB/T 7714	Musa, Zulpiya,Ma, Jinbiao,Egamberdieva, Dilfuza,et al. Diversity and Antimicrobial Potential of Cultivable Endophytic Actinobacteria Associated With the Medicinal Plant Thymus roseus[J]. 中国科学院新疆生态与地理研究所, 新疆大学,2020,11.
APA	Musa, Zulpiya.,Ma, Jinbiao.,Egamberdieva, Dilfuza.,Mohamad, Osama Abdalla Abdelshafy.,Abaydulla, Gulsumay.,...&Li, Li.(2020).Diversity and Antimicrobial Potential of Cultivable Endophytic Actinobacteria Associated With the Medicinal Plant Thymus roseus.FRONTIERS IN MICROBIOLOGY,11.
MLA	Musa, Zulpiya,et al."Diversity and Antimicrobial Potential of Cultivable Endophytic Actinobacteria Associated With the Medicinal Plant Thymus roseus".FRONTIERS IN MICROBIOLOGY 11(2020).