干旱区生态环境知识资源中心(Arid): Efficient Fingerprinting of the Tetraploid Salix psammophila Using SSR Markers

Arid

DOI	10.3390/f11020176
	Efficient Fingerprinting of the Tetraploid Salix psammophila Using SSR Markers
	Hao, Lei 1; Zhai, Yongguang 2; Zhang, Guosheng 3; Lu, Dongye 3; Huang, Haiguang 3
通讯作者	Zhang, Guosheng
来源期刊	FORESTS
EISSN	1999-4907
出版年	2020
卷号	11 期号:2
英文摘要	Salix psammophila C. Wang et Ch. Y. Yang is an important desert shrub that is mainly distributed in northwest China, including the Mu Us sandland and Kubuqi desert. It plays a crucial role in vegetation rehabilitation and as a forestation plant. The traditional identification of its accessions based on phenotypic traits is usually unreliable. SSR (Simple Sequence Repeat) has the advantages of repeatability and codominant inheritance, and most species have had specific SSR primers developed for them already. Currently, there is no simple and rapid method used for identifying the tetraploid Salix psammophila with SSR markers. In this study, we construct fingerprints among 261 accessions of S. psammophila by screening of marker combinations. We identified a nine-marker combination which could completely distinguish each of the 261 accessions to their unique fingerprinting profiles. For this marker combination (G+I+J+N+O+Q+S+T+U), identification rate of combined markers (MC2) and total Polymorphism Information Content (PIC) were the highest, at 100% and 6.05, respectively. We used fingerprinting profiles with the nine-marker combination to produce two-dimensional barcodes, which could be screened rapidly and conveniently using a barcode scanned by a computer. The results of this study can provide an efficient genetic toolkit for identification, traceability management and protection of intellectual property rights of particular accessions of tetraploid S. psammophila.
英文关键词	Salix psammophila SSR markers fingerprinting tetraploid
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000519236600017
WOS关键词	GENETIC DIVERSITY ; L. CULTIVARS ; VARIETIES ; IDENTIFICATION ; ESTABLISHMENT ; DATABASE ; PROGRAM ; PLANTS
WOS类目	Forestry
WOS研究方向	Forestry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314532
作者单位	1.Inner Mongolia Univ Finance & Econ, Coll Resources & Environm Econ, Hohhot 010070, Peoples R China; 2.Inner Mongolia Agr Univ, Water Conservancy & Civil Engn Coll, Hohhot 010019, Peoples R China; 3.Inner Mongolia Agr Univ, Coll Forestry, Hohhot 010019, Peoples R China
推荐引用方式 GB/T 7714	Hao, Lei,Zhai, Yongguang,Zhang, Guosheng,et al. Efficient Fingerprinting of the Tetraploid Salix psammophila Using SSR Markers[J],2020,11(2).
APA	Hao, Lei,Zhai, Yongguang,Zhang, Guosheng,Lu, Dongye,&Huang, Haiguang.(2020).Efficient Fingerprinting of the Tetraploid Salix psammophila Using SSR Markers.FORESTS,11(2).
MLA	Hao, Lei,et al."Efficient Fingerprinting of the Tetraploid Salix psammophila Using SSR Markers".FORESTS 11.2(2020).