干旱区生态环境知识资源中心(Arid): Dynamic expression of intra- and extra-cellular proteome and the influence of epiphytic bacteria for Nostoc flagelliforme in response to rehydration

Arid

DOI	10.1111/1462-2920.14931
	Dynamic expression of intra- and extra-cellular proteome and the influence of epiphytic bacteria for Nostoc flagelliforme in response to rehydration
	Wang, Bing; Yang, Jingjing; Xu, Chen; Yi, Lanxing; Wan, Cuihong
通讯作者	Wan, Cuihong
来源期刊	ENVIRONMENTAL MICROBIOLOGY
ISSN	1462-2912
EISSN	1462-2920
出版年	2020
卷号	22 期号:4 页码:1251-1264
英文摘要	Nostoc flagelliforme is well known for its strong ecological adaptability in inhabiting desert biological soil crusts. However, the mechanism of its recovery from quiescent to active state after prolonged dormancy remains poorly characterized. Especially how exoproteome be related to the adaptive strategies and participate in the microalgae-bacteria interaction. In the present work, we analysed the intra- and extra-cellular proteome of N. flagelliforme over a complete rehydration period both in sterilization and in natural condition for the first time. The protein expression profile for N. flagelliforme has more fluctuations during the first 1 h after wetting but been relatively steady after fully hydrated. According to the extracellular proteomic datasets, we found a dynamic secretion of various extracellular hydrolytic enzymes and membrane transport proteins, which were related to peptidoglycan digestion and nutrient exchange respectively. Two-hundred and thirteen differentially expressed proteins induced by sterilization also reflect variation in nutrient exchange and highlight symbiosis between N. flagelliforme and surrounding bacteria. We also identified 112 phosphopeptides and 217 phosphorylation site of 95 protein of hydrated N. flagelliforme. The time course datasets we present here will be a reference for understanding the molecular processes underlying N. flagelliforme resuscitation and its potential role in microbial community diversification and soil desertification control.
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000511406200001
WOS关键词	BLUE-GREEN-ALGA ; DESICCATION-TOLERANCE ; CYANOBACTERIA ; PROTEINS ; DEHYDRATION ; LIGHT ; PHOSPHORYLATION ; PEPTIDOGLYCAN ; CYANOPHYCEAE ; MICROALGAE
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314434
作者单位	Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Peoples R China
推荐引用方式 GB/T 7714	Wang, Bing,Yang, Jingjing,Xu, Chen,et al. Dynamic expression of intra- and extra-cellular proteome and the influence of epiphytic bacteria for Nostoc flagelliforme in response to rehydration[J],2020,22(4):1251-1264.
APA	Wang, Bing,Yang, Jingjing,Xu, Chen,Yi, Lanxing,&Wan, Cuihong.(2020).Dynamic expression of intra- and extra-cellular proteome and the influence of epiphytic bacteria for Nostoc flagelliforme in response to rehydration.ENVIRONMENTAL MICROBIOLOGY,22(4),1251-1264.
MLA	Wang, Bing,et al."Dynamic expression of intra- and extra-cellular proteome and the influence of epiphytic bacteria for Nostoc flagelliforme in response to rehydration".ENVIRONMENTAL MICROBIOLOGY 22.4(2020):1251-1264.