干旱区生态环境知识资源中心(Arid): Efficient Nitrogen-Fixing Bacteria Isolated from Soybean Nodules in the Semi-arid Region of Northeast Brazil are Classified as Bradyrhizobium brasilense (Symbiovar Sojae)

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DOI	10.1007/s00284-020-01993-6
	Efficient Nitrogen-Fixing Bacteria Isolated from Soybean Nodules in the Semi-arid Region of Northeast Brazil are Classified as Bradyrhizobium brasilense (Symbiovar Sojae)
	da Costa, Elaine Martins 1,2; Almeida Ribeiro, Paula R.1; de Carvalho, Teotonio Soares 1; Vicentin, Rayssa Pereira 1; Balsanelli, Eduardo 3; de Souza, Emanuel Maltempi 3; Lebbe, Liesbeth 4; Willems, Anne 4; de Souza Moreira, Fatima M.1
通讯作者	de Souza Moreira, Fatima M.
来源期刊	CURRENT MICROBIOLOGY
ISSN	0343-8651
EISSN	1432-0991
出版年	2020
卷号	77 期号:8 页码:1746-1755
英文摘要	Soybean (Glycine max L.) is an important legume that greatly benefits from inoculation with nitrogen-fixing bacteria. In a previous study, five efficient nitrogen-fixing bacterial strains, isolated from nodules of soybean inoculated with soil from semi-arid region, Northeast Brazil, were identified as a new group within the genus Bradyrhizobium. The taxonomic status of these strains was evaluated in this study. The phylogenetic analysis of the 16S rRNA gene showed the high similarity of the five strains to Bradyrhizobium brasilense UFLA03-321(T) (100%), B. pachyrhizi PAC48(T) (100%), B. ripae WR4(T) (100%), B. elkanii USDA 76(T) (99.91%), and B. macuxiense BR 10303(T) (99.91%). However, multilocus sequence analysis of the housekeeping genes atpD, dnaK, gyrB, recA, and rpoB, average nucleotide identity, and digital DNA-DNA hybridization analyses supported the classification of the group as B. brasilense. Some phenotypic characteristics allowed differentiating the five strains and the type strain of B. brasilense from the two neighboring species (B. pachyrhizi PAC48(T) and B. elkanii USDA 76(T)). The nodC and nifH genes' analyses showed that these strains belong to symbiovar sojae, together with B. elkanii (USDA 76(T)) and B. ferriligni (CCBAU 51502(T)). The present results support the classification of these five strains as Bradyrhizobium brasilense (symbiovar sojae).
类型	Article
语种	英语
国家	Brazil ; Belgium
收录类别	SCI-E
WOS记录号	WOS:000528077000001
WOS关键词	SP-NOV. ; GENETIC DIVERSITY ; ROOT-NODULES ; STRAINS ; RHIZOBIA ; LEGUMES ; DNA ; GENUS ; LEGUMINOSAE ; DEFINITION
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314303
作者单位	1.Univ Fed Lavras, Dept Ciencia Solo, BR-37200000 Lavras, MG, Brazil; 2.Univ Fed Piaui, Campus Prof Cinobelina Elvas, BR-64900000 Bom Jesus, Piaui, Brazil; 3.Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531990 Curitiba, Parana, Brazil; 4.Univ Ghent, Fac Sci, Lab Microbiol, B-9000 Ghent, Belgium
推荐引用方式 GB/T 7714	da Costa, Elaine Martins,Almeida Ribeiro, Paula R.,de Carvalho, Teotonio Soares,et al. Efficient Nitrogen-Fixing Bacteria Isolated from Soybean Nodules in the Semi-arid Region of Northeast Brazil are Classified as Bradyrhizobium brasilense (Symbiovar Sojae)[J],2020,77(8):1746-1755.
APA	da Costa, Elaine Martins.,Almeida Ribeiro, Paula R..,de Carvalho, Teotonio Soares.,Vicentin, Rayssa Pereira.,Balsanelli, Eduardo.,...&de Souza Moreira, Fatima M..(2020).Efficient Nitrogen-Fixing Bacteria Isolated from Soybean Nodules in the Semi-arid Region of Northeast Brazil are Classified as Bradyrhizobium brasilense (Symbiovar Sojae).CURRENT MICROBIOLOGY,77(8),1746-1755.
MLA	da Costa, Elaine Martins,et al."Efficient Nitrogen-Fixing Bacteria Isolated from Soybean Nodules in the Semi-arid Region of Northeast Brazil are Classified as Bradyrhizobium brasilense (Symbiovar Sojae)".CURRENT MICROBIOLOGY 77.8(2020):1746-1755.