干旱区生态环境知识资源中心(Arid): Antioxidative and Radioprotective Properties of Glycosylated Flavonoid, Xanthorhamnin from Radio-Resistant Bacterium Bacillus indicus Strain TMC-6

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DOI	10.1007/s00284-020-01930-7
	Antioxidative and Radioprotective Properties of Glycosylated Flavonoid, Xanthorhamnin from Radio-Resistant Bacterium Bacillus indicus Strain TMC-6
	Chaudhri, Ayesha Ahmed 1; Nadeem, Mahnoor 1; Rahman, Asim ur 1; Alam, Tayyaba 1; Sajjad, Wasim 2; Hasan, Fariha 1; Badshah, Malik 1; Khan, Samiullah 1; Rehman, Fazal 3; Shah, Aamer Ali 1
通讯作者	Shah, Aamer Ali
来源期刊	CURRENT MICROBIOLOGY
ISSN	0343-8651
EISSN	1432-0991
出版年	2020
卷号	77 期号:7 页码:1245-1253
英文摘要	A radio-resistant bacterium labeled as strain TMC-6 was isolated from Thal desert, Pakistan and identified through 16S rRNA gene sequencing as Bacillus indicus strain TMC-6 (MN721293). The isolate was found to be resistant to UV radiation dose of 6.780 x 10(3) J/m(2) and showed 50% survivability to mitomycin C (6 mu g/ml) and H2O2 (30 mM). The bacterium showed yellowish orange coloration when grown on tryptone yeast glucose (TGY) medium. The cellular metabolite was extracted in methanol and purified through solid phase extraction with C18 column cartridge. The compound was characterized through UV/Visible spectrophotometry, Fourier Transform Infra-Red (FT-IR) spectroscopy and Liquid Chromatography Mass Spectrometry (LC-MS). The LC-MS analysis of the compound revealed a molar mass of 769 [m/z](-) that matched the chemical formula C34H42O20 and identified as a glycosylated flavonoid xanthorhamnin. The compound showed significant antioxidant (77.05%) and metal chelation (79.80%) activities. Xanthorhamnin showed promising oxidative damage inhibitory actions in bovine serum albumin (65.32%) and mice liver lipids (71.61%) and prevented DNA strand breaks from oxidative stress. Cytotoxicity in brine shrimp larvae was observed when compared with mitomycin C indicating its effect toward cancerous cells. These findings concluded that xanthorhamnin from radio-resistant Bacillus indicus strain TMC-6 has high antioxidant, radioprotective, and antitumor properties against UV-mediated oxidative damages.
类型	Article
语种	英语
国家	Pakistan
收录类别	SCI-E
WOS记录号	WOS:000518083400003
WOS关键词	LIPID-PEROXIDATION ; AMPLIFICATION ; CANCER
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314300
作者单位	1.Quaid I Azam Univ, Fac Biol Sci, Dept Microbiol, Islamabad 45320, Pakistan; 2.Natl Univ Med Sci, Dept Biol Sci, Rawalpindi 46000, Pakistan; 3.Univ Balochistan, Dept Biotechnol, Quetta, Pakistan
推荐引用方式 GB/T 7714	Chaudhri, Ayesha Ahmed,Nadeem, Mahnoor,Rahman, Asim ur,et al. Antioxidative and Radioprotective Properties of Glycosylated Flavonoid, Xanthorhamnin from Radio-Resistant Bacterium Bacillus indicus Strain TMC-6[J],2020,77(7):1245-1253.
APA	Chaudhri, Ayesha Ahmed.,Nadeem, Mahnoor.,Rahman, Asim ur.,Alam, Tayyaba.,Sajjad, Wasim.,...&Shah, Aamer Ali.(2020).Antioxidative and Radioprotective Properties of Glycosylated Flavonoid, Xanthorhamnin from Radio-Resistant Bacterium Bacillus indicus Strain TMC-6.CURRENT MICROBIOLOGY,77(7),1245-1253.
MLA	Chaudhri, Ayesha Ahmed,et al."Antioxidative and Radioprotective Properties of Glycosylated Flavonoid, Xanthorhamnin from Radio-Resistant Bacterium Bacillus indicus Strain TMC-6".CURRENT MICROBIOLOGY 77.7(2020):1245-1253.