干旱区生态环境知识资源中心(Arid): Enhanced specificity due to method specific limits for relative ion intensities in a high-performance liquid chromatography - tandem mass spectrometry method for iohexol in human serum

Arid

DOI	10.1515/cclm-2019-1001
	Enhanced specificity due to method specific limits for relative ion intensities in a high-performance liquid chromatography - tandem mass spectrometry method for iohexol in human serum
	Schweinsberg, Vincent 1,2,3,4,5; Eckelt, Stefan 5; Schulz, Anna Marta 1,2,3,6; Arlt, Marcel 5; Schumann, Marc 5; van der Giet, Markus 1,2,3,6; Tauber, Rudolf 1,2,3,4,5; Binscheck-Domass, Torsten 5; Kiessling, Peggy 1,2,3,4,5
通讯作者	Schweinsberg, Vincent
来源期刊	CLINICAL CHEMISTRY AND LABORATORY MEDICINE
ISSN	1434-6621
EISSN	1437-4331
出版年	2020
卷号	58 期号:5 页码:709-718
英文摘要	Background: Accurate assessment of kidney function is needed for a variety of clinical indications and for research. The measurement of the serum clearance of iohexol has emerged as a feasible method to reach this objective. We report the analytical validation and clinical application of a new high-performance liquid chromatography (HPLC) - tandem mass spectrometry (MS/MS) assay to quantify iohexol in human serum. Specificity was enhanced due to the use of method specific acceptance limits for relative ion (RI) intensities. Methods: The internal standard ioversol was added to 50 mu L serum prior to protein precipitation with methanol. Linear gradient elution was performed on a Waters Oasis (R) HLB column. Three transitions for both iohexol and ioversol were monitored allowing calculation of RIs. Measurements acquired during method validation were used as a training set to establish stricter acceptance criteria for RIs which were then tested retrospectively on clinical routine measurements (86 measurements) and on mathematically simulated interferences. Results: The method was linear between 5.0 mu g/mL (lower limit of quantification [LLOQ]) and 100.3 mu g/mL iohexol. Intraday and interday imprecision were <= 2.6% and <= 3.2%, respectively. Bias was -1.6% to 1.5%. All validation criteria were met, including selectivity, recovery, extraction efficiency and matrix effects. Retrospectively acceptance limits for RIs could be narrowed to +/- 4 relative standard deviations of the corresponding RIs in the training set. The new limits resulted in an enhanced sensitivity for the simulated interferences. Conclusions: Criteria for validation were met and the assay is now used in our clinical routine diagnostics and in research.
英文关键词	glomerular filtration rate high-performance liquid chromatography iohexol kidney function relative ion intensities tandem mass spectrometry
类型	Article
语种	英语
国家	Germany
收录类别	SCI-E
WOS记录号	WOS:000523559400008
WOS关键词	GLOMERULAR-FILTRATION-RATE ; LABELED INTERNAL STANDARDS ; CHRONIC KIDNEY-DISEASE ; PLASMA-CLEARANCE ; SAMPLE SOLVENT ; RENAL-FUNCTION ; LC-MS ; IOTHALAMATE ; GFR ; QUANTIFICATION
WOS类目	Medical Laboratory Technology
WOS研究方向	Medical Laboratory Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314270
作者单位	1.Charite Univ Med Berlin, Augustenburger Pl 1, D-13353 Berlin, Germany; 2.Free Univ Berlin, Augustenburger Pl 1, D-13353 Berlin, Germany; 3.Humboldt Univ, Augustenburger Pl 1, D-13353 Berlin, Germany; 4.Berlin Inst Hlth, Inst Lab Med Clin Chem & Pathobiochem, Augustenburger Pl 1, D-13353 Berlin, Germany; 5.Charite Vivantes GmbH, Dept Lab Med & Toxicol, Lab Berlin, Sylter Str 2, D-13353 Berlin, Germany; 6.Berlin Inst Hlth, Dept Nephrol, Berlin, Germany
推荐引用方式 GB/T 7714	Schweinsberg, Vincent,Eckelt, Stefan,Schulz, Anna Marta,et al. Enhanced specificity due to method specific limits for relative ion intensities in a high-performance liquid chromatography - tandem mass spectrometry method for iohexol in human serum[J],2020,58(5):709-718.
APA	Schweinsberg, Vincent.,Eckelt, Stefan.,Schulz, Anna Marta.,Arlt, Marcel.,Schumann, Marc.,...&Kiessling, Peggy.(2020).Enhanced specificity due to method specific limits for relative ion intensities in a high-performance liquid chromatography - tandem mass spectrometry method for iohexol in human serum.CLINICAL CHEMISTRY AND LABORATORY MEDICINE,58(5),709-718.
MLA	Schweinsberg, Vincent,et al."Enhanced specificity due to method specific limits for relative ion intensities in a high-performance liquid chromatography - tandem mass spectrometry method for iohexol in human serum".CLINICAL CHEMISTRY AND LABORATORY MEDICINE 58.5(2020):709-718.