干旱区生态环境知识资源中心(Arid): Genome-wide identification and expression analysis of WRKY transcription factors in pearl millet (Pennisetum glaucum) under dehydration and salinity stress

Arid

DOI	10.1186/s12864-020-6622-0
	Genome-wide identification and expression analysis of WRKY transcription factors in pearl millet (Pennisetum glaucum) under dehydration and salinity stress
	Chanwala, Jeky 2; Satpati, Suresh 2; Dixit, Anshuman 2; Parida, Ajay 2; Giri, Mrunmay Kumar 1; Dey, Nrisingha 2
通讯作者	Giri, Mrunmay Kumar ; Dey, Nrisingha
来源期刊	BMC GENOMICS
ISSN	1471-2164
出版年	2020
卷号	21 期号:1
英文摘要	Background Plants have developed various sophisticated mechanisms to cope up with climate extremes and different stress conditions, especially by involving specific transcription factors (TFs). The members of the WRKY TF family are well known for their role in plant development, phytohormone signaling and developing resistance against biotic or abiotic stresses. In this study, we performed a genome-wide screening to identify and analyze the WRKY TFs in pearl millet (Pennisetum glaucum; PgWRKY), which is one of the most widely grown cereal crops in the semi-arid regions. Results A total number of 97 putative PgWRKY proteins were identified and classified into three major Groups (I-III) based on the presence of WRKY DNA binding domain and zinc-finger motif structures. Members of Group II have been further subdivided into five subgroups (IIa-IIe) based on the phylogenetic analysis. In-silico analysis of PgWRKYs revealed the presence of various cis-regulatory elements in their promoter region like ABRE, DRE, ERE, EIRE, Dof, AUXRR, G-box, etc., suggesting their probable involvement in growth, development and stress responses of pearl millet. Chromosomal mapping evidenced uneven distribution of identified 97 PgWRKY genes across all the seven chromosomes of pearl millet. Synteny analysis of PgWRKYs established their orthologous and paralogous relationship among the WRKY gene family of Arabidopsis thaliana, Oryza sativa and Setaria italica. Gene ontology (GO) annotation functionally categorized these PgWRKYs under cellular components, molecular functions and biological processes. Further, the differential expression pattern of PgWRKYs was noticed in different tissues (leaf, stem, root) and under both drought and salt stress conditions. The expression pattern of PgWRKY33, PgWRKY62 and PgWRKY65 indicates their probable involvement in both dehydration and salinity stress responses in pearl millet. Conclusion Functional characterization of identified PgWRKYs can be useful in delineating their role behind the natural stress tolerance of pearl millet against harsh environmental conditions. Further, these PgWRKYs can be employed in genome editing for millet crop improvement.
英文关键词	Pearl millet WRKY transcription factors Cis-regulatory elements Synteny Abiotic stress
类型	Article
语种	英语
国家	India
开放获取类型	Green Submitted, gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000520999100003
WOS关键词	PLANT-RESPONSES ; CLIMATE-CHANGE ; SEQUENCE ALIGNMENTS ; GENE FAMILY ; PROFILES ; VISUALIZATION ; SUPERFAMILY ; ANNOTATION ; DATABASE ; TOMATO
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314194
作者单位	1.KIIT Deemed Be Univ, Sch Biotechnol, Campus 11, Bhubaneswar 751024, Odisha, India; 2.Inst Life Sci, NALCO Nagar Rd,NALCO Sq, Bhubaneswar 751023, Odisha, India
推荐引用方式 GB/T 7714	Chanwala, Jeky,Satpati, Suresh,Dixit, Anshuman,et al. Genome-wide identification and expression analysis of WRKY transcription factors in pearl millet (Pennisetum glaucum) under dehydration and salinity stress[J],2020,21(1).
APA	Chanwala, Jeky,Satpati, Suresh,Dixit, Anshuman,Parida, Ajay,Giri, Mrunmay Kumar,&Dey, Nrisingha.(2020).Genome-wide identification and expression analysis of WRKY transcription factors in pearl millet (Pennisetum glaucum) under dehydration and salinity stress.BMC GENOMICS,21(1).
MLA	Chanwala, Jeky,et al."Genome-wide identification and expression analysis of WRKY transcription factors in pearl millet (Pennisetum glaucum) under dehydration and salinity stress".BMC GENOMICS 21.1(2020).