干旱区生态环境知识资源中心(Arid): Recombinant yellow protein of the takeout family and albino-related takeout protein specifically bind to lutein in the desert locust

Arid

DOI	10.1016/j.bbrc.2019.11.113
	Recombinant yellow protein of the takeout family and albino-related takeout protein specifically bind to lutein in the desert locust
	Sugahara, Ryohei 1; Tsuchiya, Wataru 2; Yamazaki, Toshimasa 2; Tanaka, Seiji 3; Shiotsuki, Takahiro 4
通讯作者	Sugahara, Ryohei ; Shiotsuki, Takahiro
来源期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
EISSN	1090-2104
出版年	2020
卷号	522 期号:4 页码:876-880
英文摘要	Yellow protein of the takeout family (YPT) and albino-related takeout protein (ALTO) are involved in body-color polyphenism in Schistocerca gregaria. YPT has been proposed to bind to beta-carotene, whereas the physiological role of ALTO is unclear. Structurally, takeout proteins contain a long continuous tunnel to bind specific ligands. However, the specific ligands of YPT and ALTO have not been fully elucidated. Here, we isolated the full coding cDNAs of these proteins and successfully produced recombinant YPT and ALTO using an Escherichia coli expression system. Absorption spectral analyses of YPT with and without carotenoids revealed that this protein bound to lutein. In contrast, obvious binding of YPT to beta-carotene and astaxanthin was not detected. Similar results were obtained for ALTO. The presence of juvenile hormone only weakly affected the protein/carotenoid interactions. These results suggested that YPT and ALTO specifically bound to lutein in a juvenile hormone-independent manner. (C) 2019 Elsevier Inc. All rights reserved.
英文关键词	Desert locust Carotenoids Phenotypic plasticity Takeout family Juvenile hormone Recombinant protein
类型	Article
语种	英语
国家	Japan
收录类别	SCI-E
WOS记录号	WOS:000524710200010
WOS关键词	BODY-COLOR POLYMORPHISM ; SCHISTOCERCA-GREGARIA ; MIGRATORIA ORTHOPTERA ; PHASE POLYPHENISM ; HORMONAL-CONTROL ; GENE ; GRASSHOPPER ; EVOLUTION ; BEHAVIOR ; NYMPHS
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314165
作者单位	1.Hirosaki Univ, Fac Agr & Life Sci, Bunkyo Cho 3, Hirosaki, Aomori 0368561, Japan; 2.Natl Agr & Food Res Org, Adv Anal Ctr, Kannondai 2-1-2, Tsukuba, Ibaraki 3058602, Japan; 3.Natl Inst Agrobiol Sci, Locust Res Lab, Tsukuba, Ibaraki 3058634, Japan; 4.Shimane Univ, Fac Life & Environm Sci, Nishikawatsu Cho 1060, Matsue, Shimane 6908504, Japan
推荐引用方式 GB/T 7714	Sugahara, Ryohei,Tsuchiya, Wataru,Yamazaki, Toshimasa,et al. Recombinant yellow protein of the takeout family and albino-related takeout protein specifically bind to lutein in the desert locust[J],2020,522(4):876-880.
APA	Sugahara, Ryohei,Tsuchiya, Wataru,Yamazaki, Toshimasa,Tanaka, Seiji,&Shiotsuki, Takahiro.(2020).Recombinant yellow protein of the takeout family and albino-related takeout protein specifically bind to lutein in the desert locust.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,522(4),876-880.
MLA	Sugahara, Ryohei,et al."Recombinant yellow protein of the takeout family and albino-related takeout protein specifically bind to lutein in the desert locust".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 522.4(2020):876-880.