干旱区生态环境知识资源中心(Arid): Streptomyces taklimakanensis sp. nov., an actinomycete isolated from the Taklimakan desert

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DOI	10.1007/s10482-020-01416-3
	Streptomyces taklimakanensis sp. nov., an actinomycete isolated from the Taklimakan desert
	Yuan, Lin-Lin; Zhang, Li-Li; Luo, Xiao-Xia; Xia, Zhan-Feng; Sun, Bei-Bei; Zeng, Hong
通讯作者	Yuan, Lin-Lin ; Zeng, Hong
来源期刊	ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN	0003-6072
EISSN	1572-9699
出版年	2020
卷号	113 期号:7 页码:1023-1031
英文摘要	A novel Streptomyces strain, designated TRM43335(T), was isolated from the Taklimakan desert in Alar City, Xinjiang, north-west China. The strain was found to exhibit an inhibitory effect on biofilm formation by Candida albicans and Staphylococcus epidermidis. A polyphasic approach was used to determine its taxonomic status. The strain was observed to form abundant aerial mycelium, occasionally twisted and which differentiated into spiral spore chains. Spores of TRM43335(T) were observed to be oval-shaped, with a smooth surface. Strain TRM43335(T) was found to grow optimally at 37 degrees C, pH 8 and in the presence of 1% (w/v) NaCl. The major sugars were identified as ribose, xylose, glucose, mannose and galactose, and the principal phospholipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. The diagnostic cell wall amino acid was identified as LL-diaminopimelic acid. The predominant menaquinone was found to be MK-9(H-6). The major cellular fatty acids were identified as iso-C-16:1 H, anteiso-C-15:0, iso-C-16:0, anteiso-C-17:0, anteiso-C-17:1 w9c and iso-C-15:0. Analysis of the 16S rRNA sequence showed that strain TRM43335(T) exhibits high sequence similarity to Streptomyces desertarenae SYSU D8023(T) 98.69%. A multilocus sequence analysis of five house-keeping genes (atpD, gyrB, rpoB, recA and trpB) also illustrated that strain TRM43335(T) should be assigned to the genus Streptomyces. The DNA G + C content of the strain was determined to be 72.8 mol%. The average nucleotide identity relatedness between strain TRM43335(T) and the phylogenetically related strain S. desertarenae SYSU D8023(T) was found to be 89.23%, and the in silico DNA-DNA hybridization value to be 36.70%. Therefore, it is concluded that strain TRM43335(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces taklimakanensis sp. nov is proposed. The type strain is TRM43335(T) (CCTCC AA 2018052( T) = KCTC 49254( T)).
英文关键词	Actinomycete Streptomyces taklimakanensis sp nov Polyphasic taxonomy Anti-biofilm
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000527451700002
WOS关键词	DNA-DNA HYBRIDIZATION ; MULTILOCUS SEQUENCE-ANALYSIS ; CLADE ; GENUS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314033
作者单位	Tarim Univ, Coll Life Sci, Xinjiang Prod & Construct Corps Key Lab Protect &, Alar 843300, Xinjiang, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Lin-Lin,Zhang, Li-Li,Luo, Xiao-Xia,et al. Streptomyces taklimakanensis sp. nov., an actinomycete isolated from the Taklimakan desert[J],2020,113(7):1023-1031.
APA	Yuan, Lin-Lin,Zhang, Li-Li,Luo, Xiao-Xia,Xia, Zhan-Feng,Sun, Bei-Bei,&Zeng, Hong.(2020).Streptomyces taklimakanensis sp. nov., an actinomycete isolated from the Taklimakan desert.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,113(7),1023-1031.
MLA	Yuan, Lin-Lin,et al."Streptomyces taklimakanensis sp. nov., an actinomycete isolated from the Taklimakan desert".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 113.7(2020):1023-1031.