干旱区生态环境知识资源中心(Arid): Raman imaging of microbial colonization in rock-some analytical aspects

Arid

DOI	10.1007/s00216-020-02622-8
	Raman imaging of microbial colonization in rock-some analytical aspects
	Vitek, Petr 1; Ascaso, Carmen 2; Artieda, Octavio 3,4; Casero, M. Cristina 2; Wierzchos, Jacek 2
通讯作者	Vitek, Petr
来源期刊	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN	1618-2642
EISSN	1618-2650
出版年	2020
卷号	412 期号:15 页码:3717-3726
英文摘要	Raman imaging allows one to obtain spatially resolved chemical information in a nondestructive manner. Herein, we present analytical aspects of effective in situ and in vivo Raman imaging of algae and cyanobacteria from within their native rock habitats. Specifically, gypsum and halite inhabited by endolithic communities from the hyperarid Atacama Desert were analyzed. Raman imaging of these phototrophic colonization reveals a pigment composition within the aggregates that helps in understanding some of their adaptation strategies to survive in this harsh polyextreme environment. The study is focused on methodical aspects of Raman imaging acquisition and subsequent data processing. Point imaging is compared with line imaging in terms of their image quality, spatial resolution, spectral signal-to-noise ratio, time requirements, and risk of laser-induced sample alteration. The roles of excitation wavelength, exposure time, and step size of the imaging grid on successful Raman imaging results are also discussed.
英文关键词	Raman mapping Geobiology Astrobiology Scytonemin Image analysis Bioimage
类型	Article
语种	英语
国家	Czech Republic ; Spain
收录类别	SCI-E
WOS记录号	WOS:000524155800001
WOS关键词	PLANT-CELL WALLS ; ATACAMA DESERT ; HALITE ; LIGNIN ; SPECTROSCOPY ; MICROFOSSILS ; COMMUNITIES ; MICROSCOPE ; CELLULOSE ; SEARCH
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/314010
作者单位	1.Czech Acad Sci, Global Change Res Inst, Belidla 986-4a, Brno 60300, Czech Republic; 2.CSIC, Museo Nacl Ciencias Nat, C Serrano 115 Dpdo, E-28006 Madrid, Spain; 3.Univ Extremadura, Dept Biol Vegetal Ecol & Ciencias Tierra, Plasencia 10600, Spain; 4.Univ Extremadura, IACYS, Plasencia 10600, Spain
推荐引用方式 GB/T 7714	Vitek, Petr,Ascaso, Carmen,Artieda, Octavio,et al. Raman imaging of microbial colonization in rock-some analytical aspects[J],2020,412(15):3717-3726.
APA	Vitek, Petr,Ascaso, Carmen,Artieda, Octavio,Casero, M. Cristina,&Wierzchos, Jacek.(2020).Raman imaging of microbial colonization in rock-some analytical aspects.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,412(15),3717-3726.
MLA	Vitek, Petr,et al."Raman imaging of microbial colonization in rock-some analytical aspects".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 412.15(2020):3717-3726.