干旱区生态环境知识资源中心(Arid): Transcriptome profiling of mild-salt responses in Lycium ruthenicum early seedlings to reveal salinity-adaptive strategies

Arid

DOI	10.1007/s11738-020-03048-6
	Transcriptome profiling of mild-salt responses in Lycium ruthenicum early seedlings to reveal salinity-adaptive strategies
	Wang, Hongbin 1; Guo, Jilin 1; Tian, Zhongping 1; Li, Jin 1; Deng, Lingfu 2; Zheng, Yong 1,2; Yuan, Yongze 2
通讯作者	Zheng, Yong ; Yuan, Yongze
来源期刊	ACTA PHYSIOLOGIAE PLANTARUM
ISSN	0137-5881
EISSN	1861-1664
出版年	2020
卷号	42 期号:4
英文摘要	The traditional Chinese desert shrub Lycium ruthenicum is widely distributed in arid environments such as north-west China, exhibiting ideal salt tolerance to cope with soil desertification, salinity, and alkalinity. However, the salt-tolerance mechanism of L. ruthenicum, especially of its young seedlings at early vegetative stages, remains largely unknown. In the present study, we collected whole-seedling samples from Lycium ruthenicum at a-pair-leaf stage with and without a mild salt (75 mM sodium chloride) treatment, and then performed transcriptome profiling to compare their gene expression patterns. The de novo assembly achieved 94,651 unigenes with 55,156 annotated. Among them, 199 differentially expressed genes (DEGs) were identified between salt-treated seedlings and control, with 41 up-regulated and 158 down-regulated. These DEGs were highly enriched into gene ontology (GO) classifications 'metabolic process' and 'catalytic activity', into Clusters of Orthologous Groups (COG) function classifications 'translation, ribosomal structure and biogenesis' and 'energy production and conversion', and into Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways 'ribosome' and 'oxidative phosphorylation'. Specifically, genes involved in energy metabolism (oxidative phosphorylation) and related energy-consuming metabolisms, including ribosome-associated biogenesis and biosynthesis of organic nitrogen-derived compatible solutes (i.e., arginine and proline), were generally down-regulated. Specific genes involved in abscisic acid (ABA) biosynthesis and signaling pathway were simultaneously up-regulated. Changes in the transcript levels of salt-responsive DEGs selected from the transcriptomic profiling were further validated by real-time quantitative polymerase chain reaction (RT-qPCR) analysis. Based on these results, salinity-adaptive strategies for the L. ruthenicum early seedlings are discussed.
英文关键词	Mild-salt stress Salinity-adaptive strategies Lycium ruthenicum Early seedlings Transcriptome
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000524561800002
WOS关键词	ABSCISIC-ACID BIOSYNTHESIS ; STRESS-ASSOCIATED PROTEIN ; ABIOTIC STRESS ; GENE-EXPRESSION ; RNA-SEQ ; 9-CIS-EPOXYCAROTENOID DIOXYGENASE ; SOLANUM-LYCOPERSICON ; ARABIDOPSIS-THALIANA ; GLUTAMINE-SYNTHETASE ; TOLERANCE
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/313888
作者单位	1.Xinjiang Normal Univ, Xinjiang Key Lab Special Species Conservat & Regu, Coll Life Sci, Urumqi 830054, Peoples R China; 2.Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China
推荐引用方式 GB/T 7714	Wang, Hongbin,Guo, Jilin,Tian, Zhongping,et al. Transcriptome profiling of mild-salt responses in Lycium ruthenicum early seedlings to reveal salinity-adaptive strategies[J],2020,42(4).
APA	Wang, Hongbin.,Guo, Jilin.,Tian, Zhongping.,Li, Jin.,Deng, Lingfu.,...&Yuan, Yongze.(2020).Transcriptome profiling of mild-salt responses in Lycium ruthenicum early seedlings to reveal salinity-adaptive strategies.ACTA PHYSIOLOGIAE PLANTARUM,42(4).
MLA	Wang, Hongbin,et al."Transcriptome profiling of mild-salt responses in Lycium ruthenicum early seedlings to reveal salinity-adaptive strategies".ACTA PHYSIOLOGIAE PLANTARUM 42.4(2020).