干旱区生态环境知识资源中心(Arid): Coexistence of Multiple Theileria annulata Genotypes Circulating in Neonatal Calves in Semi-arid India

Arid

DOI	10.2478/s11686-020-00196-0
	Coexistence of Multiple Theileria annulata Genotypes Circulating in Neonatal Calves in Semi-arid India
	Paliwal, Sanjhi; Sudan, Vikrant; Shanker, Daya; Srivastava, Mukesh
通讯作者	Sudan, Vikrant
来源期刊	ACTA PARASITOLOGICA
ISSN	1230-2821
EISSN	1896-1851
出版年	2020
卷号	65 期号:3 页码:679-685
英文摘要	Background Knowledge of local isolates and strains is a prerequisite for the development of either effective mass vaccination strategy or a suitable molecular marker-based diagnostic tool. Purpose The pathogenesis of Bovine tropical theileriosis (BTT), caused by Theileria annulata in susceptible ruminants, is known to vary depending upon the nature of isolate and strain involved. Therefore, RFLP and sequencing-based characterization of Indian isolates of T. annulata were attempted using TAMS gene. Method In the present study, TAMS 1 gene of T. annulata was amplified from 25 naturally infected calves from the BTT endemic semi-arid zone of Northern India. The amplified products were then digested with three restrictions enzymes viz., Taq I, Rsa I, and Alu I to find out the variations in pattern of restriction digests, so as to have an idea about the various isolates of T. annulata present in the studied area. Around 14 samples covering all the variants (from the PCR-RFLP patterns) were sequenced and submitted in NCBI (MH277607-MH277620). Result Coexistence of 4 variant genotypes was detected upon in-silico analysis of RFLP and sequence variations. Conclusion The nucleotide variations alongside the chromatogram analysis revealed point mutations leading to presence of noticeable genetic diversity among the isolates.
英文关键词	Genetic variations TAMS 1 gene Theileria annulata PCR-RFLP Sequencing
类型	Article
语种	英语
国家	India
收录类别	SCI-E
WOS记录号	WOS:000528505600001
WOS关键词	CATTLE ; PCR ; DSCHUNKOWSKY ; ANTIGEN ; TICKS ; LUHS
WOS类目	Parasitology ; Veterinary Sciences ; Zoology
WOS研究方向	Parasitology ; Veterinary Sciences ; Zoology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/313885
作者单位	UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vig, Mathura 281001, India
推荐引用方式 GB/T 7714	Paliwal, Sanjhi,Sudan, Vikrant,Shanker, Daya,et al. Coexistence of Multiple Theileria annulata Genotypes Circulating in Neonatal Calves in Semi-arid India[J],2020,65(3):679-685.
APA	Paliwal, Sanjhi,Sudan, Vikrant,Shanker, Daya,&Srivastava, Mukesh.(2020).Coexistence of Multiple Theileria annulata Genotypes Circulating in Neonatal Calves in Semi-arid India.ACTA PARASITOLOGICA,65(3),679-685.
MLA	Paliwal, Sanjhi,et al."Coexistence of Multiple Theileria annulata Genotypes Circulating in Neonatal Calves in Semi-arid India".ACTA PARASITOLOGICA 65.3(2020):679-685.