干旱区生态环境知识资源中心(Arid): Colocynth (Citrullus colocynthis) seed extracts attenuate adipogenesis by down-regulating PPAR gamma/SREBP-1c and C/EBP alpha in 3T3-L1 cells

Arid

DOI	10.1016/j.fbio.2019.100491
	Colocynth (Citrullus colocynthis) seed extracts attenuate adipogenesis by down-regulating PPAR gamma/SREBP-1c and C/EBP alpha in 3T3-L1 cells
	Jemai, Raoua 1,2; Drira, Riadh 2; Makni, Mohamed 1; Fetoui, Hamadi 1; Sakamoto, Kazuichi 2
通讯作者	Sakamoto, Kazuichi
来源期刊	FOOD BIOSCIENCE
ISSN	2212-4292
EISSN	2212-4306
出版年	2020
卷号	33
英文摘要	Adipogenesis is the overall process of adipocyte differentiation. With a positive energy balance, intracellular accumulation of triglyceride increases through the generation of functional adipocytes. This occurs with adipogenesis of undifferentiated preadipocytes. Citrullus colocynthis (CC), a member of the Cucurbitaceae family, is a desert viny plant native to North Africa. The effect of ethanolic seed extracts of colocynth (SCEE) on adipogenesis was investigated using 3T3-L1 preadipocytes. Cell viability was measured using the MTT assay; triglycerides were stained with Oil Red O and adipogenesis-related gene expressions were quantified using qRT-PCR. Results showed that SCEE helps to inhibit intracellular triglyceride accumulation during adipogenesis without affecting cell viability. Likewise, SCEE not only showed anti-adipogenic activities, essentially during the early stage, but their effects during the middle and late stages were very low. These effects have been confirmed by the down-regulation of C/EBP beta and C/EBP delta at 24, 48 and 72 h. In addition, SCEE treatment in 3T3-L1 cells induced down-regulation of the transcription factors CCAAT/enhancer binding protein a (C/EBP alpha), peroxisome proliferator activated receptor gamma (PPAR gamma), and sterol regulatory element-binding protein 1c (SREBP-1c) and their target genes. UPLC-ESI-MS/MS profiling showed 10 bioactive compounds including quinic acid, isovitexin, scoparin, vitexin-2 ''-O-rhamnoside, reserpine, digitoxin, triprolidine, naringenin, linoleic acid and oleic acid. Thus, these results suggested that the seeds of colocynth may have beneficial health effects.
英文关键词	Citrullus colocynthis Adipogenesis C/EBP alpha PPAR gamma SREBP-1
类型	Article
语种	英语
国家	Tunisia ; Japan
收录类别	SCI-E
WOS记录号	WOS:000504494300004
WOS关键词	MATE ILEX-PARAGUARIENSIS ; ADIPOCYTE DIFFERENTIATION ; GENE-EXPRESSION ; TRANSCRIPTION FACTORS ; FATTY-ACID ; IN-VITRO ; OBESITY ; MACROPHAGES ; FLAVONOIDS
WOS类目	Food Science & Technology
WOS研究方向	Food Science & Technology
EI主题词	2020-02-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/312741
作者单位	1.Univ Sfax, Fac Sci, Lab Toxicol Environm Microbiol & Hlth, Sfax, Tunisia; 2.Univ Tsukuba, Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan
推荐引用方式 GB/T 7714	Jemai, Raoua,Drira, Riadh,Makni, Mohamed,et al. Colocynth (Citrullus colocynthis) seed extracts attenuate adipogenesis by down-regulating PPAR gamma/SREBP-1c and C/EBP alpha in 3T3-L1 cells[J],2020,33.
APA	Jemai, Raoua,Drira, Riadh,Makni, Mohamed,Fetoui, Hamadi,&Sakamoto, Kazuichi.(2020).Colocynth (Citrullus colocynthis) seed extracts attenuate adipogenesis by down-regulating PPAR gamma/SREBP-1c and C/EBP alpha in 3T3-L1 cells.FOOD BIOSCIENCE,33.
MLA	Jemai, Raoua,et al."Colocynth (Citrullus colocynthis) seed extracts attenuate adipogenesis by down-regulating PPAR gamma/SREBP-1c and C/EBP alpha in 3T3-L1 cells".FOOD BIOSCIENCE 33(2020).