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DOI	10.1016/j.envres.2019.108891
	The effect of dust storm particles on single human lung cancer cells
	Ardon-Dryer, Karin 1,2; Mock, Caroline 1; Reyes, Jose 1; Lahav, Galit 1
通讯作者	Ardon-Dryer, Karin
来源期刊	ENVIRONMENTAL RESEARCH
ISSN	0013-9351
EISSN	1096-0953
出版年	2020
卷号	181
英文摘要	Exposure to dust particles during dust storms can lead to respiratory problems, diseases, and even death. The effect of dust particles at the cellular level is poorly understood. In this study, we investigated the impact that dust storm particles (Montmorillonite) have on human lung epithelial cells (A549) at the single cell level. Using live-cell imaging, we continuously followed individual cells after exposure to a wide range of concentrations of dust particles. We monitored the growth trajectory of each cell including number and timing of divisions, interaction with the dust particles, as well as time and mechanism of cell death. We found that individual cells show different cellular fates (survival or death) even in response to the same dust concentration. Cells that died interacted with dust particles for longer times, and engulfed more dust particles, compared with surviving cells. While higher dust concentrations reduced viability in a dose-dependent manner, the effect on cell death was nonmonotonic, with intermediate dust concentration leading to a larger fraction of dying cells compared to lower and higher concentrations. This non-monotonic relationship was explained by our findings that high dust concentrations inhibit cell proliferation. Using cellular morphological features, supported by immunoblots and proinflammatory cytokines, we determined that apoptosis is the dominant death mechanism at low dust concentrations, while higher dust concentrations activate necrosis. Similar single cell approaches can serve as a baseline for evaluating other aerosol types that will improve our understanding of the health-related consequences of exposure to dust storms.
英文关键词	Dust storm particles Single cell analysis Cell death Cell division Engulfing
类型	Article
语种	英语
国家	USA
开放获取类型	Green Accepted
收录类别	SCI-E
WOS记录号	WOS:000510109700035
WOS关键词	IN-VITRO ; PARTICULATE MATTER ; HUMAN HEALTH ; DESERT DUST ; INFLAMMATORY CYTOKINES ; DEATH ; APOPTOSIS ; RESPONSES ; TOXICITY ; EXPOSURE
WOS类目	Environmental Sciences ; Public, Environmental & Occupational Health
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
EI主题词	2020-02-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/312739
作者单位	1.Harvard Univ, Harvard Med Sch, Dept Syst Biol, 200 Longwood Ave,Warren Alpert Bldg, Boston, MA 02115 USA; 2.Texas Tech Univ, Atmospher Sci Grp, Dept Geosci, 3003 15th St, Lubbock, TX 79409 USA
推荐引用方式 GB/T 7714	Ardon-Dryer, Karin,Mock, Caroline,Reyes, Jose,et al. The effect of dust storm particles on single human lung cancer cells[J],2020,181.
APA	Ardon-Dryer, Karin,Mock, Caroline,Reyes, Jose,&Lahav, Galit.(2020).The effect of dust storm particles on single human lung cancer cells.ENVIRONMENTAL RESEARCH,181.
MLA	Ardon-Dryer, Karin,et al."The effect of dust storm particles on single human lung cancer cells".ENVIRONMENTAL RESEARCH 181(2020).