干旱区生态环境知识资源中心(Arid): Comparative antioxidant, antibacterial, and GC-MS analysis of methanol extract's fractions and isolation of luteolin from leaves of trans-Himalayan Codonopsis clematidea

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DOI	10.1016/j.indcrop.2019.112046
	Comparative antioxidant, antibacterial, and GC-MS analysis of methanol extract's fractions and isolation of luteolin from leaves of trans-Himalayan Codonopsis clematidea
	Bhardwaj, Pushpender 1; Naryal, Avilekh 1; Thakur, Mohan Singh 1; Aggarwal, Neeraj Kumar 2; Saxena, Shweta 1; Chaurasia, Om Prakash 1; Kumar, Raj 1
通讯作者	Kumar, Raj
来源期刊	INDUSTRIAL CROPS AND PRODUCTS
ISSN	0926-6690
EISSN	1872-633X
出版年	2020
卷号	144
英文摘要	Codonopsis clematidea, is a trans-Himalayan cold desert medicinal plant species, traditionally used by local tribal communities for the cure of various ailments. On this basis in present study the phytochemical composition and biological potential (antibacterial and antioxidant) of various fractions (n-hexane, chloroform, ethyl acetate, and n-butanol) of methanol extract obtained by soxhlet extraction technique from leaves of Codonopsis clematidea were investigated. Different fractions were accessed for their yield percentage, total polyphenol content (TPC), total flavonoid content (TFC), antioxidant potential (DPPH - 1,1-diphenyl-2-picrylhydrazyl; ABTS - 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), reducing power assay (FRAP - ferric reducing antioxidant power activity), and antibacterial activity (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli). Further, different fractions were also subjected to gas chromatography - mass spectrometry (GC-MS) analysis in order to assess the composition of various volatile phytoconstituents. In antioxidant assays, ethyl acetate fractions (EF) showed the strongest DPPH and ABTS antioxidant (IC50: 0.082 and 0.004 mg/mL) and FRAP potential, which significantly correlate to its high content of polyphenolics and flavonoids. However, EF has also demonstrated similar response in antibacterial activity. Further, luteolin was isolated and identified in EF using chromatography techniques and nuclear magnetic resonance (NMR) spectroscopy. The present results support the possible use of the ethyl acetate fraction from Codonopsis clematidea in food, pharmaceutical, and cosmetic industries.
英文关键词	Codonopsis clematidea Antioxidant Luteolin Antibacterial Ethyl acetate fraction
类型	Article
语种	英语
国家	India
收录类别	SCI-E
WOS记录号	WOS:000510521700020
WOS关键词	NEPETA-LEUCOPHYLLA ; AERIAL PARTS ; SOLVENTS/TECHNIQUES ; CHROMATOGRAPHY ; FLAVONOIDS
WOS类目	Agricultural Engineering ; Agronomy
WOS研究方向	Agriculture
EI主题词	2020-02-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/312737
作者单位	1.Def Inst High Altitude Res, Med Plant Div, 56 APO, Leh Ladakh, Jammu & Kashmir, India; 2.Kurukshetra Univ, Dept Microbiol, Kurukshetra 136119, Haryana, India
推荐引用方式 GB/T 7714	Bhardwaj, Pushpender,Naryal, Avilekh,Thakur, Mohan Singh,et al. Comparative antioxidant, antibacterial, and GC-MS analysis of methanol extract's fractions and isolation of luteolin from leaves of trans-Himalayan Codonopsis clematidea[J],2020,144.
APA	Bhardwaj, Pushpender.,Naryal, Avilekh.,Thakur, Mohan Singh.,Aggarwal, Neeraj Kumar.,Saxena, Shweta.,...&Kumar, Raj.(2020).Comparative antioxidant, antibacterial, and GC-MS analysis of methanol extract's fractions and isolation of luteolin from leaves of trans-Himalayan Codonopsis clematidea.INDUSTRIAL CROPS AND PRODUCTS,144.
MLA	Bhardwaj, Pushpender,et al."Comparative antioxidant, antibacterial, and GC-MS analysis of methanol extract's fractions and isolation of luteolin from leaves of trans-Himalayan Codonopsis clematidea".INDUSTRIAL CROPS AND PRODUCTS 144(2020).