干旱区生态环境知识资源中心(Arid): Biomarker-based assessment of the muscle maintenance and energy status of anurans from an extremely seasonal semi-arid environment, the Brazilian Caatinga

Arid

DOI	10.1016/j.cbpa.2019.110590
	Biomarker-based assessment of the muscle maintenance and energy status of anurans from an extremely seasonal semi-arid environment, the Brazilian Caatinga
	Madelaire, Carla Bonetti 1; Gomes, Fernando Ribeiro 1; Sokolova, Inna 2,3
通讯作者	Madelaire, Carla Bonetti
来源期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
ISSN	1095-6433
EISSN	1531-4332
出版年	2020
卷号	240
英文摘要	Strongly seasonal environments pose challenges for performance and survival of animals, especially when resource abundance seasonally fluctuates. We investigated the seasonal variation of key metabolic biomarkers in the muscles of males from three species (Rhinella jimi, R. granulosa and Pleurodema diplolister) of anurans from the drastically seasonal Brazilian semi-arid area, Caatinga. We examined the expression of proteins regulating energy turnover (AMP-activated protein kinase [AMPK] and protein kinase B [AKT]), protein synthesis and homeostasis (total and phosphorylated eukaryotic initiation factor 2 alpha [eIF2 alpha and p-eIF2 alpha] and chaperone proteins [HSP 60, 70, and 901) in muscles predominantly related to reproduction and locomotion. Cytochrome c oxidase (COX) activity was also assessed as an index of the muscle aerobic capacity. The expression pattern of metabolic biomarkers indicates that the maintenance of muscular function is regulated in a species-specific manner during the drastic seasonal variation. Rhinella jimi and R. granulosa that remain active during the drought appear to maintain muscles through more energy expensive pathways including elevated protein synthesis, while the aestivating P. diplolister employs energy conservation strategy suppressing protein synthesis, decreasing chaperone expression and increasing expression of AMPK. Two (P. diplolister and R. granulosa) of the three studied species activate cell survival pathways during the drought likely to prevent muscle atrophy, and all three studied species maintain the muscle aerobic capacity throughout the year, despite the resource limitation. These strategies are important considering the unpredictability of the reproductive event and high demand on muscular activity during the reproductive season in these amphibians. Summary statement We studied seasonal variation of key metabolic biomarkers in the muscles of anurans that experience drastic variation in environmental conditions and differ in seasonal activity patterns.
英文关键词	AKT eIF2 alpha AMPK, heat shock proteins, cytochrome c oxidase Aestivation
类型	Article
语种	英语
国家	Brazil ; USA ; Germany
开放获取类型	Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000508489600004
WOS关键词	ACTIVATED PROTEIN-KINASE ; HEAT-SHOCK PROTEINS ; SKELETAL-MUSCLE ; PLASMA-LEVELS ; METABOLIC DEPRESSION ; DISUSE ATROPHY ; FROG ; ESTIVATION ; SURVIVAL ; STRESS
WOS类目	Biochemistry & Molecular Biology ; Physiology ; Zoology
WOS研究方向	Biochemistry & Molecular Biology ; Physiology ; Zoology
EI主题词	2020-02-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/312678
作者单位	1.Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Rua Matao,Trav 14,321, BR-05508900 Sao Paulo, SP, Brazil; 2.Univ North Carolina Charlotte, Dept Biol, 9201 Univ City Blvd, Charlotte, NC 28223 USA; 3.Univ Rostock, Inst Biol Sci, Marine Biol, Rostock, Germany
推荐引用方式 GB/T 7714	Madelaire, Carla Bonetti,Gomes, Fernando Ribeiro,Sokolova, Inna. Biomarker-based assessment of the muscle maintenance and energy status of anurans from an extremely seasonal semi-arid environment, the Brazilian Caatinga[J],2020,240.
APA	Madelaire, Carla Bonetti,Gomes, Fernando Ribeiro,&Sokolova, Inna.(2020).Biomarker-based assessment of the muscle maintenance and energy status of anurans from an extremely seasonal semi-arid environment, the Brazilian Caatinga.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,240.
MLA	Madelaire, Carla Bonetti,et al."Biomarker-based assessment of the muscle maintenance and energy status of anurans from an extremely seasonal semi-arid environment, the Brazilian Caatinga".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 240(2020).