干旱区生态环境知识资源中心(Arid): Identification of drought response genes by digital gene expression (DGE) analysis in Caragana korshinskii Kom.

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DOI	10.1016/j.gene.2019.144170
	Identification of drought response genes by digital gene expression (DGE) analysis in Caragana korshinskii Kom.
	Long, Yan 1; Liang, Fengping 1,2; Zhang, Jingwen 1,2; Xue, Mande 1; Zhang, Tianbao 1; Pei, Xinwu 1
通讯作者	Pei, Xinwu
来源期刊	GENE
ISSN	0378-1119
EISSN	1879-0038
出版年	2020
卷号	725
英文摘要	Caragana korshinskii Kom. is a legume shrub that is widely distributed across desert habitats with gravely, sandy, and saline soils in Asia and Africa. C. korshinskii has highly developed roots and a strong tolerance to abiotic stress. At present, there are few genetic studies of C. korshinskii because of the limited availability of genomic resources. To understand the comprehensive mechanisms that are associated with drought tolerance, we used RNA-seq to survey the differentially expressed genes (DEGs) in comparisons of drought-treated and control plants. After analysing the sequencing results, we found 440 differentially expressed genes existing in drought-treated and control plants. Among the DEGs, 39 unigenes showed up-regulated expression after drought treatment, while 401 unigenes were down-regulated. We used the KEGG database to annotate these drought-induced genes; 126 unigenes were identified by KEGG pathway annotation, and approximately 28% of the unigenes with known function fell into categories related to fatty acid metabolism, starch, sucrose metabolism, and nitrogen metabolism, suggesting that these pathways or processes may be involved in the drought response. Finally, we confirmed that one gene has a potential function in drought tolerance. Our study is the first to provide transcriptomic resources for Caragana korshinskii and to determine its digital gene expression profile under conditions of drought stress using the assembled transcriptomic data for reference. These data provide a valuable resource for genetic and genomic studies of desert plants under abiotic stress conditions.
英文关键词	Caragana korshinskii Kom. Drought stress Digital gene expression
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000498755900004
WOS关键词	STRESS ; TOLERANCE ; TOOL
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
EI主题词	2020-01-30
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/312627
作者单位	1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China; 2.Hainan Normal Univ, Coll Life Sci, Minist Educ, Key Lab Ecol Trop Isl, Haikou 571158, Hainan, Peoples R China
推荐引用方式 GB/T 7714	Long, Yan,Liang, Fengping,Zhang, Jingwen,et al. Identification of drought response genes by digital gene expression (DGE) analysis in Caragana korshinskii Kom.[J],2020,725.
APA	Long, Yan,Liang, Fengping,Zhang, Jingwen,Xue, Mande,Zhang, Tianbao,&Pei, Xinwu.(2020).Identification of drought response genes by digital gene expression (DGE) analysis in Caragana korshinskii Kom..GENE,725.
MLA	Long, Yan,et al."Identification of drought response genes by digital gene expression (DGE) analysis in Caragana korshinskii Kom.".GENE 725(2020).