干旱区生态环境知识资源中心(Arid): The Limits, Capabilities, and Potential for Life Detection with MinION Sequencing in a Paleochannel Mars Analog

Arid

DOI	10.1089/ast.2018.1964
	The Limits, Capabilities, and Potential for Life Detection with MinION Sequencing in a Paleochannel Mars Analog
	Maggiori, Catherine 1; Stromberg, Jessica 2; Blanco, Yolanda 3; Goordial, Jacqueline 1,4; Cloutis, Edward 5; Garcia-Villadangos, Miriam 3; Parro, Victor 3; Whyte, Lyle 1
通讯作者	Whyte, Lyle
来源期刊	ASTROBIOLOGY
ISSN	1531-1074
EISSN	1557-8070
出版年	2020
卷号	20 期号:3 页码:375-393
英文摘要	No instrument capable of direct life detection has been included on a mission payload to Mars since NASA's Viking missions in the 1970s. This prevents us from discovering whether life is or ever was present on Mars. DNA is an ideal target biosignature since it is unambiguous, nonspecific, and readily detectable with nanopore sequencing. Here, we present a proof-of-concept utilization of the Oxford Nanopore Technologies (ONT) MinION sequencer for direct life detection and show how it can complement results from established space mission instruments. We used nanopore sequencing data from the MinION to detect and characterize the microbial life in a set of paleochannels near Hanksville, UT, with supporting data from X-ray diffraction, reflectance spectroscopy, Raman spectroscopy, and Life Detector Chip (LDChip) microarray immunoassay analyses. These paleochannels are analogs to martian sinuous ridges. The MinION-generated metagenomes reveal a rich microbial community dominated by bacteria and containing radioresistant, psychrophilic, and halophilic taxa. With spectral data and LDChip immunoassays, these metagenomes were linked to the surrounding Mars analog environment and potential metabolisms (e.g., methane production and perchlorate reduction). This shows a high degree of synergy between these techniques for detecting and characterizing biosignatures. We also resolved a prospective lower limit of similar to 0.001 ng of DNA required for successful sequencing. This work represents the first determination of the MinION's DNA detection limits beyond ONT recommendations and the first whole metagenome analysis of a sinuous ridge analog.
英文关键词	MinION sequencing Life detection Paleochannels Mars analog Biosignatures DNA
类型	Article
语种	英语
国家	Canada ; Australia ; Spain ; USA
开放获取类型	Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000509228300001
WOS关键词	ENHANCED RAMAN-SCATTERING ; DESERT RESEARCH STATION ; SECONDARY-STRUCTURE ; BACTERIA ; COMMUNITIES ; SOIL ; PERCHLORATE ; MICROARRAY ; SURFACE ; PHYLLOSILICATE
WOS类目	Astronomy & Astrophysics ; Biology ; Geosciences, Multidisciplinary
WOS研究方向	Astronomy & Astrophysics ; Life Sciences & Biomedicine - Other Topics ; Geology
EI主题词	2020-01-23
来源机构	Commonwealth Scientific and Industrial Research Organisation
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/312587
作者单位	1.McGill Univ, Fac Agr & Environm Sci, Dept Nat Resource Sci, 21111 Lakeshore Rd,Macdonald Stewart Bldg, Ste Anne De Bellevue, PQ H9X 3V9, Canada; 2.CSIRO Mineral Resources Flagship, Kensington, NSW, Australia; 3.CSIC, INTA, Ctr Astrobiol, Dept Mol Evolut, Madrid, Spain; 4.Bigelow Lab Ocean Sci, East Boothbay, ME USA; 5.Univ Winnipeg, Fac Sci, Dept Geog, Winnipeg, MB, Canada
推荐引用方式 GB/T 7714	Maggiori, Catherine,Stromberg, Jessica,Blanco, Yolanda,et al. The Limits, Capabilities, and Potential for Life Detection with MinION Sequencing in a Paleochannel Mars Analog[J]. Commonwealth Scientific and Industrial Research Organisation,2020,20(3):375-393.
APA	Maggiori, Catherine.,Stromberg, Jessica.,Blanco, Yolanda.,Goordial, Jacqueline.,Cloutis, Edward.,...&Whyte, Lyle.(2020).The Limits, Capabilities, and Potential for Life Detection with MinION Sequencing in a Paleochannel Mars Analog.ASTROBIOLOGY,20(3),375-393.
MLA	Maggiori, Catherine,et al."The Limits, Capabilities, and Potential for Life Detection with MinION Sequencing in a Paleochannel Mars Analog".ASTROBIOLOGY 20.3(2020):375-393.