干旱区生态环境知识资源中心(Arid): Novel insights into Dhh signaling in antler chondrocyte proliferation and differentiation: Involvement of Foxa

Arid

DOI	10.1002/jcp.29528
	Novel insights into Dhh signaling in antler chondrocyte proliferation and differentiation: Involvement of Foxa
	Ma, Li 1; Duan, Cui-Cui 2; Yang, Zhan-Qing 1; Ding, Jun-Li 1; Liu, Shu 1; Yue, Zhan-Peng 1; Guo, Bin 1
通讯作者	Yue, Zhan-Peng ; Guo, Bin
来源期刊	JOURNAL OF CELLULAR PHYSIOLOGY
ISSN	0021-9541
EISSN	1097-4652
出版年	2020
卷号	235 期号:9 页码:6023-6031
英文摘要	The desert hedgehog (Dhh) is crucial for spermatogenesis and Leydig cell differentiation, but little is known regarding its physiological function in cartilage. In this study, Dhh mRNA was abundant in antler chondrocytes, where it advanced cell proliferation concomitant with accelerated transition from the G1 to the S phase and induced elevation of the hypertrophic chondrocyte markers, Col X and Runx2. Silencing of Ptch1 resulted in appreciable Smo accumulation and enhanced rDhh stimulation of Smo, whose impediment by cyclopamine obscured the proliferative function of Dhh and alleviated its guidance of chondrocyte differentiation. Further analysis evidenced the noteworthy positive action of Smo in the bridging between Dhh and Gli transcription factors. Obstruction of Gli1 by GANT58 caused the failed stimulation of Col X and Runx2 by rDhh. Analogously, siRNA against Gli1-3 hindered chondrocyte differentiation in the context of rDhh. Simultaneously, Gli transcription factors mediated the regulation of Dhh on Foxa1, Foxa2, and Foxa3, whose knockdown impaired chondrocyte differentiation. Attenuation of Foxa antagonized the augmentation of Col X and Runx2 generated by rDhh. Collectively, Dhh signaling through its target Foxa appears to induce antler chondrocyte proliferation and differentiation.
英文关键词	antler chondrocyte Dhh signaling Foxa proliferation and differentiation
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000508216600001
WOS关键词	BINDING-PROTEIN BETA ; CELL-CYCLE ; RUNX1 EXPRESSION ; DOWNSTREAM ; REPRESSION ; OSTEOBLAST ; CANCER ; GENES ; STEPS ; GLI1
WOS类目	Cell Biology ; Physiology
WOS研究方向	Cell Biology ; Physiology
EI主题词	2020-01-21
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/312554
作者单位	1.Jilin Univ, Coll Vet Med, Changchun 130062, Peoples R China; 2.Jilin Acad Agr Sci, Inst Agrofood Technol, Changchun, Peoples R China
推荐引用方式 GB/T 7714	Ma, Li,Duan, Cui-Cui,Yang, Zhan-Qing,et al. Novel insights into Dhh signaling in antler chondrocyte proliferation and differentiation: Involvement of Foxa[J],2020,235(9):6023-6031.
APA	Ma, Li.,Duan, Cui-Cui.,Yang, Zhan-Qing.,Ding, Jun-Li.,Liu, Shu.,...&Guo, Bin.(2020).Novel insights into Dhh signaling in antler chondrocyte proliferation and differentiation: Involvement of Foxa.JOURNAL OF CELLULAR PHYSIOLOGY,235(9),6023-6031.
MLA	Ma, Li,et al."Novel insights into Dhh signaling in antler chondrocyte proliferation and differentiation: Involvement of Foxa".JOURNAL OF CELLULAR PHYSIOLOGY 235.9(2020):6023-6031.