干旱区生态环境知识资源中心(Arid): Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes

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DOI	10.1111/rda.13607
	Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes
	Wang, Qi 1; Zhang, Quanwei 2; Gan, Ze 1; Li, Haijiang 1; Yang, Yang 1; Zhang, Yong 1,2; Zhao, Xingxu 1,2
通讯作者	Zhao, Xingxu
来源期刊	REPRODUCTION IN DOMESTIC ANIMALS
ISSN	0936-6768
EISSN	1439-0531
出版年	2020
卷号	55 期号:2 页码:189-199
英文摘要	Bactrian camel is an ancient and precious species of livestock; that is, unique resources exist in the desert and have important economic and scientific value. In recent years, the number of Bactrian camels has declined sharply. Due to its long reproductive cycle and seasonal oestrus, the mechanism of oestrus is unknown. To identify candidate biomarkers of reproduction, we performed a comprehensive proteomic analysis of serum from Bactrian camel in oestrus and non-oestrus, using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with tandem mass spectrometry. We identified 359 proteins, of which 32 were differentially expressed: 11 were up-regulated and 21 were down-regulated in samples from camels in oestrus. We validated the differential expression of a subset of these proteins using qPCR and Western blot. Gene ontology annotation identified that the differentially expressed proteins function in cellular processes, metabolic processes and immune system processes. Notably, five of the differentially expressed proteins, PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2, are involved in reproductive regulatory processes in other animals. KEGG enrichment analysis demonstrated significant enrichment in several cardiac-related pathways, such as 'dilated cardiomyopathy', 'hypertrophic cardiomyopathy', 'cardiac muscle contraction' and 'adrenergic signalling in cardiomyopathy'. Our results suggest that candidate biomarker (PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2) discovery can aid in understanding reproduction in Bactrian camels. We conclude that the profiling of serum proteomes, followed by the measurement of selected proteins using more targeted methods, offers a promising approach for studying mechanisms of oestrus.
英文关键词	Bactrian camel iTRAQ Oestrus reproduction serum
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000505167900001
WOS关键词	OVULATION-INDUCING FACTOR ; BINDING-PROTEIN 4 ; SEMINAL PLASMA ; SERUM ; MECHANISM ; LEUKOCYTES ; TRANSPORT ; DEPLETION ; RECEPTOR ; REVEALS
WOS类目	Agriculture, Dairy & Animal Science ; Reproductive Biology ; Veterinary Sciences
WOS研究方向	Agriculture ; Reproductive Biology ; Veterinary Sciences
EI主题词	2020-01-02
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/312355
作者单位	1.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Gansu, Peoples R China; 2.Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qi,Zhang, Quanwei,Gan, Ze,et al. Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes[J],2020,55(2):189-199.
APA	Wang, Qi.,Zhang, Quanwei.,Gan, Ze.,Li, Haijiang.,Yang, Yang.,...&Zhao, Xingxu.(2020).Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes.REPRODUCTION IN DOMESTIC ANIMALS,55(2),189-199.
MLA	Wang, Qi,et al."Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes".REPRODUCTION IN DOMESTIC ANIMALS 55.2(2020):189-199.