干旱区生态环境知识资源中心(Arid): Development and validation of solid-phase extraction coupled with a liquid chromatography-tandem mass spectrometry method for quantitation of olanzapine in saliva

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DOI	10.1016/j.jchromb.2019.121896
	Development and validation of solid-phase extraction coupled with a liquid chromatography-tandem mass spectrometry method for quantitation of olanzapine in saliva
	Dziurkowska, Ewelina 1; Jimenez-Morigosa, Cristian 2; Lopez-Rivadulla, Manuel 2; Wesolowski, Marek 1
通讯作者	Dziurkowska, Ewelina
来源期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
EISSN	1873-376X
出版年	2020
卷号	1136
英文摘要	Olanzapine is one of the most commonly used drugs for the treatment of schizophrenia and depression of various origins. Its levels are usually measured in the blood, but the collection of this diagnostic material poses many problems. Therefore, we aimed to develop a fast and sensitive method to determine olanzapine levels in saliva, an easily available diagnostic material. To reduce the consumption of toxic solvents during analyte extraction from saliva, olanzapine was isolated by solid-phase extraction using Oasis (R) MCX cartridges. Chromatographic analysis was performed by LC-MS/MS, with C18 resin in Atlantis (R) T3 column as the stationary phase and 2 mM ammonium formate and acetonitrile as the mobile phase (flow rate of 0.25 mL/min, with elution gradient). The specificity, linearity, sensitivity, precision, accuracy, and stability of the optimized method were validated. The relative standard deviation for infra-day precision for three tested olanzapine concentrations did not exceed 12.7%; the highest accuracy value was 113.9%. The recoveries from spiked saliva samples were greater than 87.3% for the two olanzapine concentrations studied. The developed method was then used to determine olanzapine levels in human saliva obtained from 15 patients treated with different doses of olanzapine.
英文关键词	LC-MS/MS Saliva Solid-phase extraction Olanzapine
类型	Article
语种	英语
国家	Poland ; Spain
收录类别	SCI-E
WOS记录号	WOS:000509632700010
WOS关键词	HUMAN PLASMA ; LC-MS/MS ; HUMAN SERUM ; ORAL FLUID ; ANTIPSYCHOTICS ; DRUGS ; UV
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
EI主题词	2020-01-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/312329
作者单位	1.Med Univ Gdansk, Dept Analyt Chem, Gen J Hallera 107, PL-80416 Gdansk, Poland; 2.Univ Santiago Compostela, Fac Med, Inst Ciencias Forenses, Serv Toxicol, Santiago De Compostela, Spain
推荐引用方式 GB/T 7714	Dziurkowska, Ewelina,Jimenez-Morigosa, Cristian,Lopez-Rivadulla, Manuel,et al. Development and validation of solid-phase extraction coupled with a liquid chromatography-tandem mass spectrometry method for quantitation of olanzapine in saliva[J],2020,1136.
APA	Dziurkowska, Ewelina,Jimenez-Morigosa, Cristian,Lopez-Rivadulla, Manuel,&Wesolowski, Marek.(2020).Development and validation of solid-phase extraction coupled with a liquid chromatography-tandem mass spectrometry method for quantitation of olanzapine in saliva.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1136.
MLA	Dziurkowska, Ewelina,et al."Development and validation of solid-phase extraction coupled with a liquid chromatography-tandem mass spectrometry method for quantitation of olanzapine in saliva".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1136(2020).